High Throughput Extracellular Flux Analyzer
High Throughput Extracellular Flux Analyzer
批准号:
8052392
负责人:
Craig Cano Beeson
金额:
$20.52万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-07-01 至 2012-06-30
关键词:
AcidsAlcohol HepatotoxicityAnimalsBioenergeticsCell Culture TechniquesCell LineCellsConsumptionDevelopmentDiabetes MellitusDiseaseDisease modelEmerging TechnologiesEnvironmentFundingGenus HippocampusGlycolysisHeart failureIndividualLeadMacular degenerationMalignant NeoplasmsMeasurementMeasuresMetabolicMitochondriaMultiple Organ FailureMyocardial IschemiaNutrientOxidative PhosphorylationPathway interactionsPhysiologyProcessRegulationResearch PersonnelSamplingScreening procedureTimeTissue SampleTissuesTrainingUnited States National Institutes of HealthWorkextracellularinstrumentinterestnovel therapeuticsresponsesmall molecule librariesuptake
中文摘要
描述(由申请人提供):
英文摘要
DESCRIPTION (provided by applicant):
Two pathways generate the bulk of ATP in animal cells -mitochondrial oxidative phosphorylation and glycolysis. Regulation of these ATP-generating processes is of fundamental interest to biologists, but these processes are difficult to study in cell lines, primary cells or tissue samples, especially in a 96-well configuration amenable to high throughput. Recently, Seahorse Bioscience has developed the XF24 Extracellular Flux Analyzer that permits serial measurements of O2 consumption and acid formation of cells cultured in 24-well plates. The flux analyzer enabled investigators to measure repetitively and non-destructively oxidative phosphorylation, glycolysis, nutrient utilization and other processes in single cell cultures. However, the 24-well instrument does not offer sufficient throughput for primary screening of large chemical libraries. Because primary cells or tissues need to examined in a limited time period, investigators using these samples are significantly limited to the number of variables that can be examined for a given animal. Accordingly, our specific aims are to 1) acquire a Seahorse 96-well Extracellular Flux Analyzer for measurement of O2 uptake and acid release, and 2) place this instrument in a multi-user environment that provides technical support, user training and applications expertise in support of individual user projects. This emerging technology will uniquely permit NIH-funded researchers working on a variety of projects to assess bioenergetics and metabolic responses in normal physiology and models of disease, including cancer, alcoholic hepatotoxicity, macular degeneration, heart ischemia and failure, multiple organ failure and diabetes. The results of those studies could lead to the development of new therapeutic treatments for some of these diseases.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Development of a lasmiditan analogue for treatment of acute kidney injury
-
批准号:8781967
-
项目类别:
-
资助金额:$23.29万
-
财政年份:2014
-
负责人:Craig Cano Beeson
-
依托单位:
Inducers of Mitochondrial Biogenesis
-
批准号:8200080
-
项目类别:
-
资助金额:$31.21万
-
财政年份:2011
-
负责人:Craig Cano Beeson
-
依托单位:
Bioenergetics Core
-
批准号:10005395
-
项目类别:
-
资助金额:$15.32万
-
财政年份:2011
-
负责人:Craig Cano Beeson
-
依托单位:
High Throughput Mitochondrial Nephrotoxicant Assay
-
批准号:8253245
-
项目类别:
-
资助金额:$80.74万
-
财政年份:2010
-
负责人:Craig Cano Beeson
-
依托单位:
High Throughput Mitochondrial Nephrotoxicant Assay
-
批准号:8001529
-
项目类别:
-
资助金额:$26.93万
-
财政年份:2010
-
负责人:Craig Cano Beeson
-
依托单位:
High Throughput Mitochondrial Nephrotoxicant Assay
-
批准号:8334035
-
项目类别:
-
资助金额:$80.07万
-
财政年份:2010
-
负责人:Craig Cano Beeson
-
依托单位:
Optimization of dipeptide-linked benzimidazole topoisomerase 1 poisons
-
批准号:7665607
-
项目类别:
-
资助金额:$26.1万
-
财政年份:2009
-
负责人:Craig Cano Beeson
-
依托单位:
BINDING INTERACTIONS WITHIN PEPTIDE-MHC COMPLEXES
-
批准号:6386567
-
项目类别:
-
资助金额:$20.42万
-
财政年份:2000
-
负责人:Craig Cano Beeson
-
依托单位:
BINDING INTERACTIONS WITHIN PEPTIDE-MHC COMPLEXES
-
批准号:6127991
-
项目类别:
-
资助金额:$22.42万
-
财政年份:2000
-
负责人:Craig Cano Beeson
-
依托单位:
BINDING INTERACTIONS WITHIN PEPTIDE-MHC COMPLEXES
-
批准号:6690625
-
项目类别:
-
资助金额:$9.25万
-
财政年份:2000
-
负责人:Craig Cano Beeson
-
依托单位:
BINDING INTERACTIONS WITHIN PEPTIDE-MHC COMPLEXES
-
批准号:6520065
-
项目类别:
-
资助金额:$10.74万
-
财政年份:2000
-
负责人:Craig Cano Beeson
-
依托单位:
Bioenergetics Core
-
批准号:9341347
-
项目类别:
-
资助金额:$18.69万
-
财政年份:--
-
负责人:Craig Cano Beeson
-
依托单位:
Bioenergetics Core
-
批准号:9149872
-
项目类别:
-
资助金额:$18.69万
-
财政年份:--
-
负责人:Craig Cano Beeson
-
依托单位:
海外基金