TOPIC 360: A SIMPLE END-TO-END SYSTEM FOR SERIAL CELL SORTING AND MANIPULATION USING A SINGLE CONTAINER- MOONSHOT

主题 360：使用单个容器进行串行细胞排序和操作的简单端到端系统 - Moonshot

• 批准号: 10044830
• 负责人: Guixin Shi
• 金额: $ 200万
• 依托单位: DIAGNOLOGIX, LLC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-16 至 2021-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10044830
• 关键词: Cell Separation; Cell Therapy; Cells; Cyclic GMP; Disseminated Malignant Neoplasm; Environment; Immunotherapy; Memory; Metastatic Melanoma; Microbubbles; Modernization; Phase; Production; Property; Stem cells; System; T-Lymphocyte; Technology; Validation; cancer type; chemotherapy; cost effective; cost efficient; innovation; interest

Chemotherapy may control but rarely cure metastatic cancers. Remarkably, modern immunotherapies, including cell-based therapies, have cured metastatic melanomas and other types of cancers. A major challenge for all cell therapies in general is the need to develop cost-effective and efficient manufacturing and delivery capabilities. We have recently established an innovative cell isolation technology by applying targeted microbubbles, which have a number of interesting properties that are ideal for bulk cell isolation. In Phase I, we have demonstrated the feasibility for manufacturing a simple and cost-efficient system to enrich cells of interest that require multiple markers to identify. In Phase II, we will continue developing a closed automated system using only a single container to be integrated in a cGMP environment for cell isolation, expansion and manipulation.

化疗可以控制但很少治愈转移性癌症。值得注意的是，现代免疫疗法，包括基于细胞的疗法，已经治愈了转移性黑色素瘤和其他类型的癌症。一般来说，所有细胞疗法面临的一个主要挑战是需要开发具有成本效益和高效的制造和输送能力。我们最近通过应用靶向微泡建立了一种创新的细胞分离技术，该技术具有许多有趣的特性，非常适合批量细胞分离。在第一阶段，我们已经证明了制造一种简单且具有成本效益的系统的可行性，该系统可以丰富需要多种标记物来识别的感兴趣细胞。在第二阶段，我们将继续开发一种封闭的自动化系统，该系统仅使用一个容器集成在cGMP环境中，用于细胞分离、扩增和操作。