IGF::OT::IGF SBIR Topic 360: A Simple End-to-end System for Serial Cell Sorting and Manipulation Using a Single ContainerSBIR Phase I Topic 360POP: 9/19/17 ¿ 6/18/18

化疗可以控制但很少治愈转移性癌症。值得注意的是，现代免疫疗法，包括基于细胞的疗法，已经治愈了转移性黑色素瘤和其他类型的癌症。一般来说，所有细胞疗法面临的一个主要挑战是需要开发具有成本效益和高效的制造和输送能力。我们最近通过应用靶向微泡建立了一种创新的细胞分离技术，该技术具有许多有趣的特性，非常适合批量细胞分离。在这里，我们提出了一个简单而经济的系统，通过整合这些特征来丰富需要多个标记来识别的感兴趣的细胞。该项目成功后，将开发和制造一个仅使用单个容器的封闭自动化系统，用于细胞分离、扩增和操作。

Chemotherapy may control but rarely cure metastatic cancers. Remarkably, modern immunotherapies, including cell-based therapies, have cured metastatic melanomas and other types of cancers. A major challenge for all cell therapies in general is the need to develop cost-effective and efficient manufacturing and delivery capabilities. We have recently established an innovative cell isolation technology by applying targeted microbubbles, which have a number of interesting properties that are ideal for bulk cell isolation. Here we propose a simple and cost-efficient system to enrich cells of interest that require multiple markers to identify by integrating these features. When this project is successful, a closed automated system using only a single container will be developed and manufactured for cell isolation, expansion and manipulation.