Development of a tool to examine proteomic pathways in the AD brain

开发一种工具来检查 AD 大脑中的蛋白质组通路

• 批准号: 10010721
• 负责人: ERIK C GUNTHER
• 金额: $ 25.19万
• 依托单位: PROTEOWISE, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2020-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10010721
• 关键词: 3-Dimensional; Acceleration; Alzheimer' s Disease; Alzheimer' s disease brain; Alzheimer' s disease therapeutic; Antibodies; Archives; Brain; Brain Diseases; Buffers; Clinical Trials; Communities; Complex; Data; Detection; Development; Diffusion; Disease; Drug Design; Electrophoresis; Elements; Engineering; Etiology; Exhibits; Gel; Human; Impairment; Individual; Legal patent; Lightning; Membrane; Methods; Miniaturization; Molecular Weight; Nuclear Pore Complex; Pathway interactions; Performance; Phase; Plague; Polyacrylamide Gel Electrophoresis; Process; Protein Analysis; Proteins; Proteomics; Research; Research Personnel; Resolution; Role; Running; Sampling; Speed; Surface; System; Techniques; Technology; Temperature; Therapeutic; Time; Translations; Universities; Validation; Western Blotting; Width; career; cost; cost effectiveness; design; drug discovery; flexibility; instrument; miniaturize; mouse model; polyacrylamide; pre-clinical; seal; success; therapeutic development; tool; voltage

Abstract For the extent of our careers as Alzheimer’s researchers, we have struggled with the limitations imposed by the standard techniques for protein analysis. Western blot, the go-to protein technology of the bench researcher, is no match for the complex brain protein distortions of AD. To remedy this debility, we devised a technology that we are making available to all researchers through the company we founded, ProteoWise Inc. ProteoWise is engineering an instrument that will provide the same protein relative concentration and molecular weight information of Western blot, 10 x faster, 10 x cheaper, using 10x less sample, and, critically, will provide unlimited multiplexed proteomic data per sample. To meet the promise of this technology, we invented micro polyacrylamide electrophoresis (mPAGE), a process we seek to optimize with the support applied for here. When complete, this instrument will not only make our AD research more successful, it will empower AD research around the world in our battle against this enemy.

摘要 作为阿尔茨海默氏症研究者，我们一直在努力克服 蛋白质分析的标准技术。蛋白质印迹， 技术的板凳研究人员，是没有匹配的复杂的大脑蛋白质扭曲的AD。 为了弥补这一缺陷，我们设计了一项技术，我们正在向所有研究人员提供 通过我们创立的公司ProteoWise Inc. ProteoWise正在设计一种仪器， 这将提供相同的蛋白质相对浓度和分子量信息， 蛋白质印迹，10倍更快，10倍更便宜，使用10倍更少的样品，而且，关键的是，将提供 每个样品无限多路复用蛋白质组数据。为了实现这项技术的承诺，我们 发明了微聚丙烯酰胺电泳（mPAGE），我们寻求优化的过程， 在这里申请的支持。完成后，该仪器不仅可以使我们的AD研究 更成功的是，它将使全世界的广告研究在我们与这个敌人的战斗中更加强大。