Specialized junctions in the development of epithelia and neural circuits
上皮细胞和神经回路发育中的特殊连接
基本信息
- 批准号:10040885
- 负责人:
- 金额:$ 21.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-01 至 2022-07-31
- 项目状态:已结题
- 来源:
- 关键词:Abnormal CellActinsAdherens JunctionAdhesionsAffectBindingCell AdhesionCell Adhesion MoleculesCell CommunicationCellsChickensComplexDendritesDetectionDevelopmentDiffusionDiseaseDrosophila genusEpithelialEpithelial CellsEpitheliumFamily memberGastrointestinal tract structureGenetic DiseasesGeometryGoalsHomologous GeneImmunoglobulinsInflammatory Bowel DiseasesIntercellular JunctionsInterneuronsInvertebratesInvestigationKidneyKnowledgeLearningLinkLocationMaintenanceMechanicsMediatingMicrofilamentsMicroscopyNatureNeoplasm MetastasisNervous system structureNeuronsOrganPDZ proteinPhasePopulationProteinsResearchRetinaRoleSeptateSkinStructureSynapsesSystemTestingTight JunctionsTimeTissuesVisual MotionVisual system structureYeastsafadinautism spectrum disorderdevelopmental diseaseimprovedinsightneural circuitpresynapticrecruitscreeningsealsolutesynaptogenesisthree dimensional structuretumoryeast two hybrid system
项目摘要
Summary
Adhesion between cells is important for the development of both epithelial tissues and neural
circuits, and a growing number of adhesion molecules have been found to act in both contexts. For
example, Sidekicks (Sdks) are immunoglobulin family members that mediate adhesion between specific
synaptic partners in the vertebrate retina. Drosophila Sdk was recently shown to act as a hub for the
assembly of tricellular adherens junctions (tAJs), which control tension at points where three epithelial cells
meet, in addition to its function in the visual motion detection circuit. This proposal will investigate the
mechanisms of Sdk localization and function in both contexts and explore possible connections between
them. The first aim will investigate whether the localization of Sdk to tAJs is driven by the fit between its
three-dimensional structure and their geometry. Sdk has been shown to recruit Polychaetoid and Canoe,
which anchor the ends of actin filaments, to tAJs. However, Sdk can also interact with the WAVE regulatory
complex, which promotes actin branching. The hypothesis that this interaction underlies the effect of Sdk on
junction lengthening, while Polychaetoid and Canoe promote junction shortening, will be tested. A yeast
two-hybrid screen will be used to identify additional factors that are recruited to tAJs by Sdk. The second
aim will examine the synaptic localization of Sdk in the visual system. Expansion microscopy will be used to
determine whether Sdk localizes to specific subregions of synapses between the Tm9 and T5 neurons,
and/or to a specific location on T5 dendrites. The effect on Sdk localization of structural alterations in the
protein and of tension in T5 will be tested. Finally, the possibility that epithelial interaction partners of Sdk
also colocalize and function with it at synapses will be investigated. This exploratory proposal will provide
insight into the assembly and function of the newly discovered tAJs, and may reveal a parallel structure at
synapses. Identifying the common features of epithelial and synaptic junctions will contribute to our
understanding of developmental disorders that affect either or both tissue types.
总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jessica E Treisman其他文献
Jessica E Treisman的其他文献
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{{ truncateString('Jessica E Treisman', 18)}}的其他基金
Mechanisms of development of curved refractive surfaces
弯曲折射表面的发展机制
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10624979 - 财政年份:2022
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$ 21.19万 - 项目类别:
Mechanisms of development of curved refractive surfaces
弯曲折射表面的发展机制
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10443019 - 财政年份:2022
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$ 21.19万 - 项目类别:
Diversification of cell types in the Drosophila retina - Resubmission - 1
果蝇视网膜细胞类型的多样化 - 重新提交 - 1
- 批准号:
10328555 - 财政年份:2021
- 资助金额:
$ 21.19万 - 项目类别:
Specialized junctions in the development of epithelia and neural circuits
上皮细胞和神经回路发育中的特殊连接
- 批准号:
10221016 - 财政年份:2020
- 资助金额:
$ 21.19万 - 项目类别:
Interactive Processes in Photoreceptor Axon Targeting
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10633287 - 财政年份:2019
- 资助金额:
$ 21.19万 - 项目类别:
Interactive processes in photoreceptor axon targeting
光感受器轴突靶向中的交互过程
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10183353 - 财政年份:2019
- 资助金额:
$ 21.19万 - 项目类别:
Interactive processes in photoreceptor axon targeting
光感受器轴突靶向中的交互过程
- 批准号:
10412062 - 财政年份:2019
- 资助金额:
$ 21.19万 - 项目类别:
Interactive processes in photoreceptor axon targeting
光感受器轴突靶向中的交互过程
- 批准号:
9796954 - 财政年份:2019
- 资助金额:
$ 21.19万 - 项目类别:
Interactive processes in photoreceptor axon targeting
光感受器轴突靶向中的交互过程
- 批准号:
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- 资助金额:
$ 21.19万 - 项目类别:
Interactive processes in photoreceptor axon targeting
光感受器轴突靶向中的交互过程
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10162404 - 财政年份:2019
- 资助金额:
$ 21.19万 - 项目类别:
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