Deactivation Mechanisms of Rod Phototransduction

杆状光转导的失活机制

• 批准号: 10039505
• 负责人: MARIE E BURNS
• 金额: $ 16.03万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10039505
• 关键词: Address; Affect; Behavioral; Biochemical; Biological; Calcium; Cations; Cell Compartmentation; Cells; Cyclic GMP; Disease; Electrodes; Electrophysiology (science); Eye; Goals; Health; Image; Individual; Interruption; Ion Channel Gating; Kinetics; Knockout Mice; Knowledge; Light; Light Adaptations; Mammals; Measures; Mediating; Mediator of activation protein; Melatonin; Membrane; Membrane Potentials; Metabolic stress; Methods; Mus; Neurons; Output; Phosphorylation; Photons; Photoreceptors; Phototransduction; Physiology; Potassium Channel; Predisposition; Process; Property; Protein Dephosphorylation; Recovery; Research; Research Personnel; Retina; Retinal Cone; Retinal Diseases; Risk Factors; Rod; Rod Outer Segments; Role; Signal Transduction; Stress; Suction; Synapses; Techniques; Technology; Time; Transgenic Mice; Vertebrate Photoreceptors; Vision; Visual; Work; circadian; circadian pacemaker; circadian regulation; experimental study; in vivo; light intensity; overexpression; photoreceptor degeneration; programs; response; retinal rods; temporal measurement; voltage

Project Summary Retinal rod and cone photoreceptor cells mediate the essential first steps in vision by signaling light intensity and its modulation. Although the initial biochemical steps that transduce light into electrical signals in the outer segments of photoreceptors are well understood, much less is known about how outer segment currents are transformed by voltage-sensitive conductances and influenced by adaptation and circadian regulation in vivo. The work proposed in this application will investigate photoreceptor physiology the living eye and the mechanisms by which photoreceptor physiology is modulated to influence visual sensitivity and kinetics, and to mitigate photoreceptor stress. Using primarily a combination of ex vivo and in vivo electrophysiological approaches, three aims will be investigated: 1. Determine the role of Kv2.1 channels in rods. 2. Define the mechanisms that constrain photoreceptor sensitivity and kinetics in vivo. 3. Determine how photoreceptor properties are modulated by the circadian clock. ! ! !

项目摘要 视网膜视杆细胞和视锥感光细胞通过 信号光强及其调制。虽然最初的生化步骤是转译 在光感受器的外部节段，光转化为电信号是众所周知的， 关于外段电流是如何通过电压敏感型进行转换的，我们知之甚少 电导，并受体内适应和昼夜节律的影响。这项工作 建议在这项应用中研究光感受器生理学，活眼和 光感受器生理调节以影响视觉敏感度和 动力学，并缓解光感受器压力。主要使用体外和体内的组合 活体电生理方法，将研究三个目的：1.确定其作用 Kv2.1通道，以棒为单位。2.定义限制光感受器敏感度和 体内动力学。3.确定昼夜节律是如何调节感光细胞特性的 钟。 好了！ 好了！ 好了！

项目成果

Biological Role of Arrestin-1 Oligomerization.

Arrestin-1 寡聚化的生物学作用。

• DOI: 10.1523/jneurosci.0749-20.2020
• 发表时间: 2020
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Samaranayake,Srimal;Vishnivetskiy,SergeyA;Shores,CamillaR;Thibeault,KimberlyC;Kook,Seunghyi;Chen,Jeannie;Burns,MarieE;Gurevich,EugeniaV;Gurevich,VsevolodV
• 通讯作者: Gurevich,VsevolodV

Recoverin regulates light-dependent phosphodiesterase activity in retinal rods.

• DOI: 10.1085/jgp.200308994
• 发表时间: 2004-06
• 期刊: The Journal of general physiology
• 作者: Makino CL;Dodd RL;Chen J;Burns ME;Roca A;Simon MI;Baylor DA
• 通讯作者: Baylor DA

Harnessing the Sun to See Anew.

利用太阳重新审视。

• DOI: 10.1016/j.neuron.2019.05.051
• 发表时间: 2019
• 期刊: Neuron
• 影响因子: 16.2
• 作者: PeinadoAllina,Gabriel;Burns,MarieE
• 通讯作者: Burns,MarieE

Lessons from photoreceptors: turning off g-protein signaling in living cells.

• DOI: 10.1152/physiol.00001.2010
• 发表时间: 2010-04
• 期刊: Physiology (Bethesda, Md.)
• 作者: Burns ME;Pugh EN Jr
• 通讯作者: Pugh EN Jr

What are the effects of severe visual impairment on the cortical organization and connectivity of primary visual cortex?

• DOI: 10.3389/neuro.05.030.2009
• 发表时间: 2009-12-23
• 期刊: FRONTIERS IN NEUROANATOMY
• 影响因子: 2.9
• 作者: Larsen, DeLaine D.;Luu, Julie D.;Krubitzer, Leah
• 通讯作者: Krubitzer, Leah