Einstein's Nathan Shock Center of Excellence in Basic Biology of Aging

摘要 衰老核心蛋白(PAC)是一个资源，研究人员感兴趣的变化研究的变化 爱因斯坦-内森休克中心(E-NSC)和其他地方衰老和老年相关疾病中的蛋白质稳定 提供最先进的方法学来研究蛋白质平衡网络成分的变化。这个 PAC为全球105个组织提供了917项服务，出版了42份出版物，生成了23份数据 批准申请并促成了许多协作项目。这个核心的具体目标是：1) 进行全面和高度专门化的检测，以研究自噬和其他蛋白平衡 成分；2)分发经过验证的试剂、样品和用于蛋白质稳定性研究的方案；3)提供 蛋白质平衡分析的实验设计、技术、程序和数据解释方面的建议 开发蛋白抑制剂靶向干预措施；4)开发和调整最先进的方法学 蛋白质平衡研究；5)促进对老年研究人员进行研究方法和程序的培训 蛋白质平衡，以及5)通过与NSC的整合成为蛋白质平衡信息的来源 协调中心和来自其他NSC核心的信息。 核心的活动分为四组：1)服务：全面的化验组合，以 不同自噬途径的蛋白质周转、活性及蛋白酶体和溶酶体功能的研究 将军。2)资源：提供经过验证的试剂、样本和方案，用于研究 衰老。3)咨询和培训：包括与PAC专家举行会议，以进行实验规划、数据 解释和药物设计，以及与技术人员的实践培训课程。4)创新：探索、 测试和实施研究蛋白质动态平衡的程序，重点是自噬途径。 人事：1)主任(Cuervo博士)监督核心的所有活动，提供建议，协助取得成果 他还负责口译，并与咨询委员会沟通，以确定优先次序/工作流程模式。2) 两名专家提供蛋白质抑制方面的建议，进行形态计量分析，并监督实施 新程序，并就药物设计、干预的实验设计和解释提供建议 结果。3)PAC技术员执行作为一种服务提供的化验，为培训和保证做出贡献 设备和库存的维护。相关性：独特的服务和持续的技术实施 而核心提供的资源使我们能够全面定量分析蛋白质平衡的变化 老龄化、老年性障碍及不同干预措施的效果。程序和程序的集中 资源优化了成本效率，并确保标准化、验证和严格的质量控制。

Summary The Proteostasis of Aging Core (PAC) is a resource for investigators interested in the study of changes in proteostasis in aging and age-related disorders at the Einstein-Nathan Shock Center (E-NSC) and elsewhere by providing state-of-the-art methodology for the study of changes in components of the proteostasis network. The PAC has provided 917 services for 105 groups worldwide, contributed to 42 publications, generated data for 23 grant applications and catalyzed numerous collaborative projects. The Specific Aims of this Core are to: 1) perform comprehensive and highly specialized assays for the study of autophagy and other proteostasis components; 2) distribute validated reagents, samples and protocols for the study of proteostasis; 3) provide advice on experimental design, techniques, procedures and data interpretation for proteostasis analysis and for development of proteostasis targeting interventions; 4) develop and adapt state-of-the-art methodology for the study of proteostasis; 5) contribute to training of aging investigators in methods and procedures for the study of proteostasis, and 5) become a resource for information in proteostasis through integration with the NSC coordinating center and with information from other NSC cores. The activities of the Core are organized into four groups: 1) Service: comprehensive battery of assays to study protein turnover, activities of the different autophagic pathways and proteasome and lysosomal function in general. 2) Resources: provides validated reagents, samples and protocols for the study of proteostasis in aging. 3) Consultation and training: includes meetings with PAC experts for experimental planning, data interpretation and drug design, and hands-on training sessions with the technician. 4) Innovation: explores, tests and implements procedures for the study of protein homeostasis with emphasis on autophagic pathways. Personnel: 1) Director (Dr. Cuervo) supervises all activities of the Core, offers advice, assists with results interpretation and communicates with the Advisory Committee to establish prioritization/ workflow patterns. 2) Two experts provide advice on proteostasis, perform morphometric analysis and supervises implementation of new procedures and provide advice on drug design, experimental design of interventions and interpretation of results. 3) The PAC technician performs assays offered as a service, contributes to training and assures maintenance of equipment and stocks. Relevance: Unique services and continuous actualization of technology and resources offered by the Core allow for comprehensive quantitative analysis of changes in proteostasis in aging, age-related disorders and the of the effect of different interventions. Centralization of procedures and resources optimizes cost efficiency and guarantees standardization, validation and tight quality control.