The Midwest Center for Cryo-Electron Tomography

中西部冷冻电子断层扫描中心

• 批准号: 10054846
• 负责人: ELIZABETH R WRIGHT
• 金额: $ 833.56万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10054846
• 关键词: Administrator; Biochemistry; Biological Sciences; Carbon; Cell Culture Techniques; Complex; Computers; Cryo-electron tomography; Cryoelectron Microscopy; Data Collection; Database Management Systems; Development; Devices; Discipline; Electron Microscope; Electron Microscopy; Ensure; Equipment; Fluorescence; Freezing; Grant; Human Resources; Image; Image Analysis; Individual; International; Ions; Laboratory culture; Light Microscope; Methods; Midwestern United States; Molecular Biology; Organelles; Organism; Postdoctoral Fellow; Preparation; Recording of previous events; Research; Research Personnel; Resources; Robot; Role; Sampling; Scanning; Scientist; Services; Site; Specimen; Standardization; System; Teacher Professional Development; Technology; Tissues; Training; Training Activity; United States National Institutes of Health; Universities; Validation; Wisconsin; computerized data processing; cryogenics; data acquisition; evaporation; experience; image processing; improved; innovation; instrumentation; macromolecule; materials science; member; operation; preservation; pressure; public health relevance; recruit; skills; small molecule; technology development; virtual

Modified Project Summary/Abstract Section We propose to establish a National Service Network Hub for Cryo-Electron Tomography to be hosted in the Department of Biochemistry at the University of Wisconsin, Madison. The Midwest Center for Cryo-Electron Tomography (MCCET) will support investigators by providing access to well-trained staff and state-of-the-art equipment for (1) routine and advanced cryo-electron tomography (cryo-ET) specimen preparation, data collection, and computation; and (2) hands-on, remote, and virtual training in cryo-ET specimen preparation, data collection, and data processing and validation. MCCET operations will build on the Cryo-EM Research Center (CEMRC) established in the Department of Biochemistry at the University of Wisconsin, Madison. The University of Wisconsin, Madison has a long history of institutional commitment to electron microscopy, as well as the advancement of electron microscopy in both the biological and materials sciences. The ancillary equipment and major instrumentation to be housed in the MCCET will include: cell culture equipment, a fluorescence light microscope and micropatterning system, glow discharge devices, carbon evaporator, automated plunge freezer, high pressure freezer, cryo-ultramicrotome, a cryo-focused ion beam-scanning electron microscope (cryo-FIB-SEM), and a 300 kV TEM. Dr. Elizabeth R. Wright, the contact PI, has over twenty-years of cryo-EM experience including the development of new cryo-EM technologies and workflows along with directing and managing (cryo-)electron microscopy research resources. Drs. Marisa Otegui, Robert Kirchdoerfer, and Tim Grant bring complementary expertise in sample preservation and imaging of complex tissues, small molecules and organelles, imaging and computational technology development, as well as facility management. A center manager, a computer scientist/systems administrator, a project manager, cryo- ET training instructors, a computer scientist, and postdoctoral research associates will manage MCCET day-today operations and support users through the course of their projects from initial access, sample handling and preparation, sample validation, cryo-ET data collection and processing, and cross-training. Hub coordination activities with the NIH, network centers, and users will be managed by the PI, project manager, center manager, and computer scientist/systems administrator. The Midwest Center for Cryo-Electron Tomography (MCCET) will maintain a culture of innovation and technology development in cryo-ET to facilitate improvements to cryo-ET sample preparation, throughput, validation, and imaging. The MCCET will be a user-experience focused resource that will provide access and training to researchers across many disciplines.

修改的项目摘要/摘要部分 我们建议建立一个国家服务网络枢纽，用于冷冻电子层析成像，将在麦迪逊大学威斯康星大学生物化学系主持。中西部冷冻电子层析成像中心（MCCET）将通过提供训练有素的员工和最先进的设备来支持研究人员（1）（1）常规和高级冷冻电子断层扫描（Cryo-ET）标本准备，数据收集和计算； （2）在冷冻标本准备，数据收集以及数据处理和验证中的动手，远程和虚拟培训。 MCCET运营将建立在威斯康星大学麦迪逊分校生物化学系建立的冷冻EM研究中心（CEMRC）上。威斯康星大学麦迪逊分校对电子显微镜的机构承诺有着悠久的历史，以及在生物学和材料科学中的电子显微镜的进步。 The ancillary equipment and major instrumentation to be housed in the MCCET will include: cell culture equipment, a fluorescence light microscope and micropatterning system, glow discharge devices, carbon evaporator, automated plunge freezer, high pressure freezer, cryo-ultramicrotome, a cryo-focused ion beam-scanning electron microscope (cryo-FIB-SEM), and a 300 kV TEM.联系PI的Elizabeth R. Wright博士拥有二十多年的低温EM经验，包括开发新的Cryo-EM技术和工作流程以及指导和管理（Cryo-）电子显微镜研究资源。博士。 Marisa Otegui，Robert Kirchdoerfer和Tim Grant在复杂组织，小分子和细胞器，成像和计算技术开发以及设施管理方面具有互补的专业知识。中心经理，计算机科学家/系统管理员，项目经理，冷冻培训讲师，计算机科学家和博士后研究协会将通过初始访问，样本处理和制备，样本验证，冷冻数据收集和处理以及交叉处理的项目来管理MCCET日期运营，并通过其项目的过程来支持用户。与NIH，网络中心和用户的集线器协调活动将由PI，项目经理，中心经理和计算机科学家/系统管理员管理。中西部冷冻电子断层扫描中心（MCCET）将维持Cryo-ET中创新和技术开发的文化，以促进改进冷冻ET样品制备，吞吐量，验证和成像。 MCCET将是以用户体验为中心的资源，将为许多学科的研究人员提供访问和培训。