Administrative Supplement: The Midwest Center for Cryo-Electron Tomography

行政补充：中西部冷冻电子断层扫描中心

• 批准号: 10817476
• 负责人: ELIZABETH R WRIGHT
• 金额: $ 337.48万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10817476
• 关键词: Administrative Supplement; Administrator; Biochemistry; Biological Sciences; Carbon; Cell Culture Techniques; Complex; Computers; Cryo-electron tomography; Cryoelectron Microscopy; Data Collection; Database Management Systems; Development; Devices; Discipline; Electron Microscope; Electron Microscopy; Ensure; Equipment; Fluorescence; Grant; Human Resources; Image; Image Analysis; Individual; Institution; International; Ions; Laboratory culture; Light Microscope; Methods; Midwestern United States; Molecular Biology; Organelles; Organism; Pattern; Postdoctoral Fellow; Preparation; Recording of previous events; Research; Research Personnel; Resources; Robot; Role; Sampling; Scanning; Scientist; Services; Site; Specimen; Standardization; System; Teacher Professional Development; Technology; Tissues; Training; Training Activity; United States National Institutes of Health; Universities; Validation; Wisconsin; computerized data processing; cryogenics; data acquisition; evaporation; experience; image processing; improved; innovation; instrumentation; macromolecule; materials science; member; operation; preservation; pressure; public health relevance; recruit; skills; small molecule; technology development; virtual

Modified Project Summary/Abstract Section We propose to establish a National Service Network Hub for Cryo-Electron Tomography to be hosted in the Department of Biochemistry at the University of Wisconsin, Madison. The Midwest Center for Cryo-Electron Tomography (MCCET) will support investigators by providing access to well-trained staff and state-of-the-art equipment for (1) routine and advanced cryo-electron tomography (cryo-ET) specimen preparation, data collection, and computation; and (2) hands-on, remote, and virtual training in cryo-ET specimen preparation, data collection, and data processing and validation. MCCET operations will build on the Cryo-EM Research Center (CEMRC) established in the Department of Biochemistry at the University of Wisconsin, Madison. The University of Wisconsin, Madison has a long history of institutional commitment to electron microscopy, as well as the advancement of electron microscopy in both the biological and materials sciences. The ancillary equipment and major instrumentation to be housed in the MCCET will include: cell culture equipment, a fluorescence light microscope and micropatterning system, glow discharge devices, carbon evaporator, automated plunge freezer, high pressure freezer, cryo-ultramicrotome, a cryo-focused ion beam-scanning electron microscope (cryo-FIB-SEM), and a 300 kV TEM. Dr. Elizabeth R. Wright, the contact PI, has over twenty-years of cryo-EM experience including the development of new cryo-EM technologies and workflows along with directing and managing (cryo-)electron microscopy research resources. Drs. Marisa Otegui, Robert Kirchdoerfer, and Tim Grant bring complementary expertise in sample preservation and imaging of complex tissues, small molecules and organelles, imaging and computational technology development, as well as facility management. A center manager, a computer scientist/systems administrator, a project manager, cryo- ET training instructors, a computer scientist, and postdoctoral research associates will manage MCCET day-today operations and support users through the course of their projects from initial access, sample handling and preparation, sample validation, cryo-ET data collection and processing, and cross-training. Hub coordination activities with the NIH, network centers, and users will be managed by the PI, project manager, center manager, and computer scientist/systems administrator. The Midwest Center for Cryo-Electron Tomography (MCCET) will maintain a culture of innovation and technology development in cryo-ET to facilitate improvements to cryo-ET sample preparation, throughput, validation, and imaging. The MCCET will be a user-experience focused resource that will provide access and training to researchers across many disciplines.

修改后的项目摘要/摘要部分 我们建议建立一个冷冻电子断层扫描国家服务网络中心，由威斯康星大学麦迪逊分校生物化学系托管。中西部冷冻电子断层扫描中心 (MCCET) 将通过提供训练有素的工作人员和最先进的设备来支持研究人员：(1) 常规和先进的冷冻电子断层扫描 (cryo-ET) 标本制备、数据收集和计算； (2) 冷冻电子断层扫描标本制备、数据收集、数据处理和验证方面的动手、远程和虚拟培训。 MCCET 运营将建立在威斯康星大学麦迪逊分校生物化学系设立的冷冻电镜研究中心 (CEMRC) 的基础上。威斯康星大学麦迪逊分校在电子显微镜领域的机构承诺以及电子显微镜在生物和材料科学领域的进步有着悠久的历史。 MCCET 中的辅助设备和主要仪器将包括：细胞培养设备、荧光显微镜和微图案系统、辉光放电装置、碳蒸发器、自动投入式冷冻机、高压冷冻机、冷冻超薄切片机、冷冻聚焦离子束扫描电子显微镜 (cryo-FIB-SEM) 和 300 kV TEM。联系人 PI Elizabeth R. Wright 博士拥有二十多年的冷冻电镜经验，包括开发新的冷冻电镜技术和工作流程以及指导和管理（冷冻）电子显微镜研究资源。博士。 Marisa Otegui、Robert Kirchdoerfer 和 Tim Grant 在复杂组织、小分子和细胞器的样本保存和成像、成像和计算技术开发以及设施管理方面带来了互补的专业知识。中心经理、计算机科学家/系统管理员、项目经理、cryo-ET 培训讲师、计算机科学家和博士后研究员将管理 MCCET 的日常运营，并在项目过程中为用户提供支持，包括初始访问、样本处理和准备、样本验证、cryo-ET 数据收集和处理以及交叉培训。与 NIH、网络中心和用户的中心协调活动将由 PI、项目经理、中心经理和计算机科学家/系统管理员管理。中西部冷冻电子断层扫描中心 (MCCET) 将保持冷冻电子断层扫描的创新和技术开发文化，以促进冷冻电子断层扫描样品制备、通量、验证和成像的改进。 MCCET 将是一个注重用户体验的资源，将为多个学科的研究人员提供访问和培训。