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Administrative Supplement: The Midwest Center for Cryo-Electron Tomography

行政补充：中西部冷冻电子断层扫描中心

基本信息

批准号：
10457095
负责人：
ELIZABETH R WRIGHT
金额：
$ 45.48万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-15 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10457095
关键词：
Administrative Supplement Administrator Biochemistry Biological Sciences Carbon Cell Culture Techniques Complex Computers Cryo-electron tomography Cryoelectron Microscopy Data Collection Database Management Systems Development Devices Discipline Electron Microscope Electron Microscopy Ensure Equipment Fluorescence Freezing Grant Human Resources Image Image Analysis Individual International Ions Laboratory culture Light Microscope Methods Midwestern United States Molecular Biology Organelles Organism Postdoctoral Fellow Preparation Recording of previous events Research Research Personnel Resources Robot Role Sampling Scanning Scientist Services Site Specimen Standardization System Teacher Professional Development Technology Tissues Training Training Activity United States National Institutes of Health Universities Validation Wisconsin computerized data processing cryogenics data acquisition evaporation experience image processing improved innovation instrumentation macromolecule materials science member operation preservation pressure public health relevance recruit skills small molecule technology development virtual

项目摘要

Modified Project Summary/Abstract Section We propose to establish a National Service Network Hub for Cryo-Electron Tomography to be hosted in the Department of Biochemistry at the University of Wisconsin, Madison. The Midwest Center for Cryo-Electron Tomography (MCCET) will support investigators by providing access to well-trained staff and state-of-the-art equipment for (1) routine and advanced cryo-electron tomography (cryo-ET) specimen preparation, data collection, and computation; and (2) hands-on, remote, and virtual training in cryo-ET specimen preparation, data collection, and data processing and validation. MCCET operations will build on the Cryo-EM Research Center (CEMRC) established in the Department of Biochemistry at the University of Wisconsin, Madison. The University of Wisconsin, Madison has a long history of institutional commitment to electron microscopy, as well as the advancement of electron microscopy in both the biological and materials sciences. The ancillary equipment and major instrumentation to be housed in the MCCET will include: cell culture equipment, a fluorescence light microscope and micropatterning system, glow discharge devices, carbon evaporator, automated plunge freezer, high pressure freezer, cryo-ultramicrotome, a cryo-focused ion beam-scanning electron microscope (cryo-FIB-SEM), and a 300 kV TEM. Dr. Elizabeth R. Wright, the contact PI, has over twenty-years of cryo-EM experience including the development of new cryo-EM technologies and workflows along with directing and managing (cryo-)electron microscopy research resources. Drs. Marisa Otegui, Robert Kirchdoerfer, and Tim Grant bring complementary expertise in sample preservation and imaging of complex tissues, small molecules and organelles, imaging and computational technology development, as well as facility management. A center manager, a computer scientist/systems administrator, a project manager, cryo- ET training instructors, a computer scientist, and postdoctoral research associates will manage MCCET day-today operations and support users through the course of their projects from initial access, sample handling and preparation, sample validation, cryo-ET data collection and processing, and cross-training. Hub coordination activities with the NIH, network centers, and users will be managed by the PI, project manager, center manager, and computer scientist/systems administrator. The Midwest Center for Cryo-Electron Tomography (MCCET) will maintain a culture of innovation and technology development in cryo-ET to facilitate improvements to cryo-ET sample preparation, throughput, validation, and imaging. The MCCET will be a user-experience focused resource that will provide access and training to researchers across many disciplines.

修改的项目摘要/摘要部分