Ambient Air Pollution, Stress, Oxidative Markers and Respiratory Health in Mexican Children

项目总结 我是一名环境流行病学家，主要研究发育窗口的作用， 多重接触的联合影响和产前/早期接触的分子机制 化学和非化学应激源影响儿童的肺部发育。这项提议的目标是 获得所需技能方面的培训，通过将环境空气污染和 应激对肺发育的影响，并确定这些暴露的早期生物影响的生物标志物。 正式课程和Rosalind Wright博士、Andrea Baccarelli博士、Robert Wright博士的专家指导， 玛丽亚·玛莎·特莱兹-罗霍、布伦特·库尔、阿尔芬·维森西奥和艾伦将使我获得这些知识 以及成为一名独立的跨学科研究人员所需的技能。这次训练将使我能够 实现我的长期职业目标：在正在进行的墨西哥城率先研究呼吸健康 进展队列，以检查这些和其他环境暴露，它们的生物学 机制，以及随后对肺功能和哮喘风险的后续影响 你什么时候才能长大。所有相关的暴露和协变量数据都存在于现有的表型良好的妊娠队列中 [肥胖、生长、环境和社会压力源的规划研究(进展)研究 在这里。我会在队列中加入呼吸道表型。具体地说，我将在最先进的压力下进行训练 RJ Wright博士的评估以及胎儿和儿童早期肺发育和生理 在巴卡雷利博士实验室的氧化应激早期生物标记物中，3)用先进的统计方法训练 每天到每周评估的时间分辨暴露数据，以确定易感窗口 与Brent Coull博士一起增强我们发现影响和识别弱势群体的能力，4)整合研究 压力、空气污染和分子流行病学通过与我的指导团队的互动成为 跨学科的科学家和翻译我接受的研究和管理培训，以确立自己的地位 作为一名独立研究员，拥有终身教职，并为未来的R01定位。 这项提议将解决对空气污染的时间和机制的理解上的差距。 暴露和压力会影响儿童早期的呼吸健康。这项拟议的研究和培训计划 为独立的研究生涯奠定了基础，旨在阐明 环境空气污染和压力与呼吸道发病率有关，并最终导致 干预/预防工作。这项研究也极具成本效益，因为我们利用 利用现有生物样本、环境和心理社会应激数据进行的进展研究。

PROJECT SUMMARY I am an environmental epidemiologist with a primary research interest in the role of developmental windows, joint impact of multiple exposures and molecular mechanisms through which prenatal/early-life exposure to chemical and non-chemical stressors influence children's lung development. The goal of this proposal is to obtain training in the skills needed to continue to build my academic career by linking ambient air pollution and stress to lung development and to identify biomarkers of early biological effects of these exposures. Formal coursework and the expert mentorship of Drs. Rosalind Wright, Andrea Baccarelli, Robert Wright, Maria Martha Tellez-Rojo, Brent Coull, Alfin Vicencio and Allan Just will enable me to acquire the knowledge and skills necessary to become an independent transdisciplinary researcher. This training will allow me to achieve my long-term career goals: taking the lead in studying respiratory health in the ongoing Mexico City PROGRESS cohort in order to examine these and other environmental exposures, their biological mechanisms, and the subsequent effects on lung function and asthma risk longitudinally as these children grow up. All relevant exposure and covariate data exist in an existing well-phenotyped pregnancy cohort [Programming Research in Obesity, Growth, Environment and Social Stressors (PROGRESS) study leveraged herein. I will add respiratory phenotyping to the cohort. Specifically I will 1) train in state of the art stress assessment as well as fetal and early childhood lung development and physiology with Dr. RJ Wright, 2) train in early biologic markers of oxidative stress in Dr. Baccarelli's lab, 3) train in advanced statistical methods that temporal resolved daily to weekly assessed exposure data to definitively identify susceptibility windows to enhance our ability to find effects and identify vulnerable groups with Dr. Brent Coull, 4) integrate research on stress, air pollution and molecular epidemiology through interactions with my mentorship team to become an interdisciplinary scientist and 5) translate the research and administrative training I receive to establish myself as a independent investigator with a tenure track faculty position and to position myself for a future R01. This proposal will address gaps in the understanding of timing and mechanism through which air pollution exposure and stress affect respiratory health in early childhood. This proposed research and training plan builds the foundation for an independent research career that aims to clarify the mechanisms through which ambient air pollution and stress are associated with respiratory morbidity and ultimately inform intervention/prevention efforts. This study is also highly cost effective as we leverage the resources of the PROGRESS study with existing biospecimens, environmental and psychosocial stress data.