UNC Neuroscience Center Research Cores

《北卡罗来纳大学神经科学中心研究核心》(P30 NS045892-14)的目的是提供NINDS资助 以及其他NINDS优先调查人员，他们的研究能力无法得到 单独的实验室。在过去的13年里，NS045892资助的核心几乎支持所有NINDS- 资助了北卡罗来纳大学教堂山医学院的研究。在这段时间里，北卡罗来纳大学的神经学家利用了这些 促进NINDS神经遗传学、疼痛、神经退行性变、 和系统神经科学。 对于我们的竞争更新，我们提出了两个核心：显微镜和生物信息学。我们定期升级 我们的显微镜核心利用更高的灵敏度、扫描速度和平铺功能，现在 超分辨率功能。除了蔡司LSM 780和其他显微镜外，我们的显微镜核心还将容纳 一款新的蔡司LSM 880，带有AiryScan和快速模块。核心还将提供IT基础设施和管道 用于计算密集型图像处理和分析薄片显微镜数据。对于 生物信息学核心，我们将资助两名生物信息学家分析高通量测序(HTS)数据， 包括外显子组和基因组测序，散装和单细胞RNA-seq，散装和单细胞ATAC-seq，Hi-C， 和芯片序列数据。 获得先进的显微成像和生物信息学支持对于切割- NINDS优先领域的前沿研究。在持续的支持下，我们的NINDS资助和其他NINDS优先事项 调查人员将通过方便和负担得起的方式访问这些密钥，从而实现他们的变革性想法 技术。

The purpose of “UNC Neuroscience Center Research Cores” (P30 NS045892-14) is to provide NINDS-funded and other NINDS-priority investigators with research capabilities that cannot be supported or sustained by individual laboratories. For the past 13 years, the Cores funded by NS045892 supported almost all NINDS- funded research at the UNC–Chapel Hill School of Medicine. During this time, UNC neuroscientists utilized these Cores to facilitate breakthrough discoveries in NINDS priority areas of neurogenetics, pain, neurodegeneration, and systems neuroscience. For our competitive renewal, we propose two Cores: Microscopy and Bioinformatics. We regularly upgraded our Microscopy Core to take advantage of increased sensitivity, scanning speeds, tiling capabilities, and now super-resolution capabilities. In addition to a Zeiss LSM 780 and other scopes, our Microscopy Core will house a new Zeiss LSM 880 with Airyscan and Fast module. The Core will also provide IT infrastructure and a pipeline for computationally intensive image processing and analysis of light sheet microscopy data. For the Bioinformatics Core, we will fund two bioinformaticians to analyze high-throughput sequencing (HTS) data, including exome and genome sequencing, bulk and single-cell RNA-seq, bulk and single-cell ATAC-seq, Hi-C, and ChIP-seq data. Access to advanced microscopic imaging and bioinformatics support is essential to the performance of cutting- edge research in NINDS-priority areas. With continued support, our NINDS-funded and other NINDS-priority investigators will have their transformative ideas enabled via convenient and affordable access to these key technologies.