Ultra-low attachment coatings to maintain CSF shunt patency

超低附着涂层可保持脑脊液分流管的通畅

• 批准号: 10077986
• 负责人: Eric Stephen Guire
• 金额: $ 23.2万
• 依托单位: INNOVATIVE SURFACE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077986
• 关键词: Achievement; Address; Adherence; Adhesions; Adsorption; Affect; American; Animal Model; Astrocytes; Biocompatible Materials; Biological; Bioreactors; Brain; Brain Death; Brain Injuries; Caliber; Catheters; Cell Adhesion; Cell surface; Cell-Matrix Junction; Cells; Cerebrospinal Fluid; Cerebrospinal Fluid Pressure; Cerebrospinal fluid shunts procedure; Childhood; Cicatrix; Deposition; Disease; Embolism; Environment; Ependymal Cell; Evaluation; Failure; Fibroblasts; Formulation; Frequencies; Gliosis; Goals; Government; Healthcare Systems; Hydrocephalus; Hydrophobicity; Implant; In Vitro; Infection; Inflammation; Inflammatory Response; Investigation; Left; Life; Mechanics; Medical Device; Mesenchymal Stem Cells; Methods; Microglia; Modification; Nature; Neuraxis; Obstruction; Operative Surgical Procedures; Organoids; Outcome; Patients; Peritoneum; Persons; Phase; Population; Procedures; Process; Property; Proteins; Rattus; Recovery; Shunt Device; Silicones; Site; Stromal Neoplasm; Structure of choroid plexus; Surface; Suspensions; System; Techniques; Technology; Testing; Time; Tissues; Tube; United States; Ventricular; Work; astrogliosis; base; biomaterial compatibility; brain cell; brain tissue; cost; culture plates; design; implantable device; in vivo; manufacturing scale-up; novel; pressure; prevent; recruit; surgical pain; surgical risk

Project Summary Over 700,000 Americans require cerebrospinal fluid (CSF) shunts to relieve excess cerebrospinal fluid pressure; however, >30% of these shunts fail within 2 years requiring multiple revision surgeries. Common causes of CSF shunt failure include aseptic clogging, infection and mechanical failure. Aseptic clogging is responsible for the majority of the failures, but is unaddressed in the marketplace. Passivation of silicone shunts against cell and tissue adhesion, as well as initial protein deposition and denaturation, is an attractive strategy to reduce the embolism and tissue ingrowth that underlies the frequency of revision surgery in hydrocephalus patients. This proposal examines a novel coating for the silicone shunts derived from ultralow attachment multiwell plates for organoids. We propose to develop a biocompatible surface-modification process for silicone implantables that, unlike other methods of silicone surface-modification, can bond permanently with silicone and resist hydrophobic recovery, a common occurrence whereby small silicone oligomers migrate through a coating to cover the surface again with silicone. In addition, the coating will resist >99% of cellular adhesion of CNS cells and tissues. To validate the technology and establish proof-of-principal, coated shunt tips will be evaluated for adhesion of microglia and astrocytes, brain tissue fragments, and choroid plexus tissue sheets in a hydrocephalus shunt catheter bioreactor system to mimic in vivo use.

项目总结