Single clone discovery using an image-based cell isolation platform

使用基于图像的细胞分离平台发现单克隆

基本信息

批准号：
10080442
负责人：
QI ZHAO
金额：
$ 84.69万
依托单位：
ENRICH THERAPEUTICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-14 至 2022-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10080442
关键词：
Address Algorithms Antibodies B-Lymphocytes Bacteriophages Biological Assay Blood Cells Cell Communication Cell Separation Cells Cellular biology Chromatin Classification Clone Cells Computer software Computers Cytotoxic T-Lymphocytes Development Developmental Biology Devices Excision Genetic Heterogeneity Goals Heterogeneity Hospitals Image Immune Immunology Methods Microfluidics Microscope Morphology Neoplasm Circulating Cells Neurobiology Organelles Pattern Recognition Phagocytosis Phase Phenotype Population Production Proteins Resistance Resolution Slide Sorting - Cell Movement T-Lymphocyte Technology Testing Time Tumor Tissue Validation anticancer research base biomaterial compatibility cell behavior commercialization computerized tools cost cost effective design experimental study high resolution imaging interest liquid crystal operation parallel computer particle photopolymerization prototype synaptogenesis tumor

项目摘要

Abstract Characterizing both functional and genetic heterogeneity among a pool of cells remains a major scientific challenge in immunology, cancer research, neurobiology and developmental biology. Isolating cells with the same phenotype is key to understand such heterogeneity. We would like to develop a non-fluidic, unique technology, LCD aided selection under microscope (LASUM, Fig.1) to address this need. During the phase I of this project, we completed the alpha version of prototype design and demonstrated its utility in three proof-of-concept experiments: (a) blood cell removal; (b) phage enrichment on single beads; (c) isolation of tumor-killing immune cell clones. We would like to continue our effort to bring this technology to market, which will be low-cost, high throughput, debris resistant, image-based with operation simplicity as preparing a microscope slide or washing a microtiter plate. Besides the device, we will unify the fragmentated cell isolation market using two associated kits: Enrich-Live and Enrich- Seq. The successful commercialization of this device/kits will enable thousands of cell- biology labs and hospitals in manipulating single cells on a computer screen.

抽象的表征一个细胞池之间的功能和遗传异质性免疫学，癌症研究，神经生物学和发展生物学。用相同表型隔离细胞是理解的关键这种异质性。我们想开发一种非富集的独特技术，LCD 在显微镜下的辅助选择（Lasum，图1）以满足此需求。在该项目的第一阶段，我们完成了alpha版本的原型设计和在三个概念验证实验中证明了其效用：（a）去除血细胞；（b）单珠上的噬菌体富集；（c）分离肿瘤免疫细胞克隆。我们想继续我们将这项技术推向市场的努力，这将是低成本的，高吞吐量，抗碎片，基于图像的基于图像的简单性，以准备显微镜滑动或清洗微量滴定板。除了设备，我们还将统一使用两个相关试剂盒进行破碎的细胞隔离市场：富集和富集 seq。该设备/套件的成功商业化将使数千个细胞能够在计算机屏幕上操纵单细胞的生物学实验室和医院。