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Single clone discovery using an image-based cell isolation platform

使用基于图像的细胞分离平台发现单克隆

基本信息

批准号：
10239253
负责人：
QI ZHAO
金额：
$ 82.91万
依托单位：
ENRICH THERAPEUTICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-14 至 2022-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10239253
关键词：
Address Algorithms Antibodies B-Lymphocytes Bacteriophages Biological Assay Blood Cells Cell Communication Cell Separation Cells Cellular biology Chemoresistance Chromatin Classification Clone Cells Computer software Computers Cytotoxic T-Lymphocytes Development Developmental Biology Devices Excision Genetic Heterogeneity Goals Heterogeneity Hospitals Image Immune Immunology Methods Microfluidics Microscope Morphology Neoplasm Circulating Cells Neurobiology Organelles Pattern Recognition Phagocytosis Phase Phenotype Population Production Proteins Resistance Resolution Slide Sorting - Cell Movement T-Lymphocyte Technology Testing Time Tumor Tissue Validation anticancer research base biomaterial compatibility cell behavior commercialization computerized tools cost cost effective design experimental study high resolution imaging interest liquid crystal operation parallel computer particle photopolymerization prototype synaptogenesis tumor

项目摘要

Abstract Characterizing both functional and genetic heterogeneity among a pool of cells remains a major scientific challenge in immunology, cancer research, neurobiology and developmental biology. Isolating cells with the same phenotype is key to understand such heterogeneity. We would like to develop a non-fluidic, unique technology, LCD aided selection under microscope (LASUM, Fig.1) to address this need. During the phase I of this project, we completed the alpha version of prototype design and demonstrated its utility in three proof-of-concept experiments: (a) blood cell removal; (b) phage enrichment on single beads; (c) isolation of tumor-killing immune cell clones. We would like to continue our effort to bring this technology to market, which will be low-cost, high throughput, debris resistant, image-based with operation simplicity as preparing a microscope slide or washing a microtiter plate. Besides the device, we will unify the fragmentated cell isolation market using two associated kits: Enrich-Live and Enrich- Seq. The successful commercialization of this device/kits will enable thousands of cell- biology labs and hospitals in manipulating single cells on a computer screen.

摘要表征细胞池中的功能和遗传异质性仍然存在免疫学、癌症研究、神经生物学和发育生物学分离具有相同表型的细胞是理解这种异质性。我们想开发一种非流体的，独特的技术，LCD 在显微镜下辅助选择（LASUM，图1），以满足这一需求。期间在这个项目的第一阶段，我们完成了原型设计的alpha版本，在三个概念验证实验中证明了其效用：（a）血细胞去除;（B）噬菌体富集在单个珠上;（c）分离肿瘤杀伤免疫细胞克隆。我们我们希望继续努力将这项技术推向市场，这将是低成本的，高通量、抗碎片、基于图像且操作简单，显微镜载玻片或洗涤微量滴定板。除了设备，我们将统一使用两种相关的试剂盒：Enrich-Live和Enrich-Like， Seq.该设备/试剂盒的成功商业化将使数千个细胞- 生物实验室和医院在计算机屏幕上操纵单细胞。