SBIR PHASE I TOPIC #439 - ADVANCED SAMPLE PROCESSING PLATFORMS FOR DOWNSTREAM SINGLE-CELL MULTI-OMIC ANALYSIS

该提案的长期目标是开发一种快速，免提平台，该平台集成了组织解离，克隆表征和分离，以支持单细胞多组学的不断发展领域。在该提案的第一阶段，enrich将通过开发用于原位组织解离、多标准单细胞分离和无损单细胞检索的基本试剂、协议和耗材，来适应现有的基于图像的抗碎片克隆分离机。组织消化、单细胞捕获、清洗和回收的效率将通过现有的实时成像功能进行严格监控。将从人肝脏和胰腺肿瘤中分离足够且高活力的单细胞，并通过10xGenomics平台制备每个样品的scRNA和scDNA测序文库，并提交用于Illumina测序。验证将通过一系列指标实现，包括总操作时间、手动干预水平、单细胞产量和质量以及sc序列可映射性。如果成功，下一阶段将进行多中心肿瘤类型研究。

The long term goal of this proposal is to develop a fast, hands-free platform that integrates tissue dissociation, clone characterization and isolation to support the growing field of single celll multiomics. During the phase I of the proposal, enrich will adapt the exiting image based, debris-resistant clone isolation machine by developing essential reagents, protocols and consumables for in-place tissue dissociation, multicriteria single cell isolation and lossless single cell retrieval. Efficiency of tissue digestion, single cell capture, washing and retrieval will be tightly monitored by existing real-time imaging functionality. Adequate and high viability single cells from human liver and pancreas tumors will be isolated and scRNA and scDNA sequencing libraries of each sample will be prepared by 10xGenomics platform and submitted for Illumina sequencing. The validation will be achieved by an array of metrics including total operation time, level of manual intevention, single cell yield and quality and sc sequence mappability. if successful, a multi-site, tumor type study will be performed in the next phase.