Characterizing the Pan-genome of a Rickettsia Infecting the Eastern Black-legged Tick
感染东部黑腿蜱的立克次体的全基因组特征
基本信息
- 批准号:10115952
- 负责人:
- 金额:$ 24.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-22 至 2022-12-31
- 项目状态:已结题
- 来源:
- 关键词:AbbreviationsAddressAdultAllelesAnaplasma phagocytophilumAnaplasmosisAntibioticsAreaArthropodsBabesiaBabesiosisBacteriaBiologyBiotinBlack-legged TickBorreliaCell LineCellsCharacteristicsChromosomesCommunicable DiseasesDataDeer TickDiseaseDisease VectorsDistantElementsEvaluationFlavivirusFrequenciesFutureGene ExpressionGenesGenetic VariationGenomeGenomicsGeographyGoalsHealthHorizontal Gene TransferHumanInfectionInheritedLife StyleLyme DiseaseMaintenanceMediatingMetabolicMicrobeMobile Genetic ElementsNamesNatureNutrientNymphParasitesPathogenesisPathogenicityPeptide SynthesisPopulationPowassan virusPrevalenceProcessProteinsPseudogenesResearchResistanceResolutionResourcesRickettsiaRickettsia rickettsiiRocky Mountain Spotted FeverRoleSTEM researchShapesSpecialistTestingTheileriaTheileriasisTick-Borne DiseasesTicksUnited StatesVertebratesWorkcombatcontigdeep sequencingdesignendosymbiontexperimental studyfeedinggenetic elementgenetic variantgenomic variationhost microbiotahuman diseasehuman pathogeninfection rateinnovationinsightmicrobialnovelpan-genomeparasitismpathogenreference genomereproductivespreading factorsuperinfectiontick populationtick-bornetooltranscriptome sequencingvector transmissionvector-borne pathogen
项目摘要
PROJECT SUMMARY
Microbial residents of arthropod disease vectors engender profound effects on the biology of their host, both
positive (e.g., nutrient acquisition, defense from pathogens) and negative (e.g., reproductive parasitism). The
mechanisms that underpin these microbial processes arise as evolutionary consequences of genetic variation and
are often mediated by mobile genetic elements (MGEs). Even when these resident microbes are not known to be
pathogenic, their impact on host biology can influence the distribution and transmission of vector-borne pathogens.
The deer tick, Ixodes scapularis, is widely distributed in the Eastern United States and is an important transmitter of
several human pathogens, including Borrelia species (Lyme disease) and Anaplasma phagocytophilum
(anaplasmosis). Deer ticks also harbor a prevalent intracellular bacterium, Rickettsia buchneri, that is unique among
Rickettsia species in several ways: 1) it is vertically inherited with high efficiency in the deer tick, but does not cycle
between ticks and vertebrates in nature; 2) it is, astonishingly, the only Rickettsia species that has ever been detected
in I. scapularis; and 3) its genome is substantially enriched with pseudogenes and MGEs that carry intriguing cargo,
including genes for biotin synthesis, antibiotic synthesis and resistance, and nonribosomal peptide synthesis. Given
its maintenance in deer ticks, and its arsenal of potential functions, R. buchneri stands to exert significant influence
on the biology of this important disease vector.
The long-term goal of this research is to determine the nature of the relationship between R. buchneri and I.
scapularis. The current work is designed to advance this research goal by addressing significant challenges; namely,
the lack of a high-quality reference genome, inconsistencies in infection rate and distribution data, and a dearth of
information regarding strain-level genomic variation. The central hypothesis of this project is that high-resolution
data on infection rate and genomic population substructure will elucidate R. buchneri's trajectory toward an obligate
endosymbiotic lifestyle. The proposed work will gauge the extent of species-specific innovation in R. buchneri by
using 1) long-read sequencing to generate a closed genome, 2) RNA-seq to confirm pseudogene prediction, and 3)
phylogenomics to characterize genes and other genomic elements unique to R. buchneri (AIM 1). The current
proposal work will also characterize the R. buchneri pan-genome by using 1) quantitative PCR to assess its infection
rate among natural populations of deer ticks, 2) short-read deep sequencing to determine the distribution of R.
buchneri genetic variants, alleles, and MGEs, and 3) phylogenomics to characterize the extent (and origin) of lateral
gene transfers into R. buchneri (AIM 2). Illuminating R. buchneri genomic variation and tick infection frequency will
lead to insights into its relationship with the deer tick and ultimately inform future efforts to use its repertoire of MGEs
as gene drive tools for spreading factors to combat tick-borne diseases.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Timothy P Driscoll其他文献
Timothy P Driscoll的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Timothy P Driscoll', 18)}}的其他基金
Characterizing the Pan-genome of a Rickettsia Infecting the Eastern Black-legged Tick
感染东部黑腿蜱的立克次体的全基因组特征
- 批准号:
10331878 - 财政年份:2021
- 资助金额:
$ 24.38万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 24.38万 - 项目类别:
Research Grant