Investigate the molecular mechanisms of the interplay between cell metabolism and histone modification in ethylene signaling in Arabadopsis

研究拟南芥乙烯信号传导中细胞代谢与组蛋白修饰相互作用的分子机制

• 批准号: 10117420
• 负责人: Hong Qiao
• 金额: $ 33.5万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10117420
• 关键词: Acetyl Coenzyme A; Anabolism; Arabidopsis; Binding; Biochemical; Cell Nucleus; Cells; Cellular Metabolic Process; Chromatin; Chromatin Structure; Cleaved cell; Complex; Cues; DNA Sequence; Data; Disease; Endoplasmic Reticulum; Environment; Enzymes; Epigenetic Process; Ethylenes; Eukaryota; Fruit; Functional disorder; Funding; Gene Expression; Gene Expression Regulation; Genetic; Genetic Diseases; Genetic Transcription; Genome; Genomic approach; Germination; Goals; Histone Acetylation; Histones; Hormones; Life Cycle Stages; Link; Mediating; Membrane; Metabolic; Metabolic Diseases; Metabolic dysfunction; Metabolism; Mitochondria; Modeling; Modification; Molecular; Nuclear; Nuclear Translocation; Organism; Phenotype; Plant Leaves; Plants; Play; Process; Protein Dephosphorylation; Proteins; Pyruvate; Pyruvate Dehydrogenase Complex; Regulation; Role; Seedling; Seeds; Signal Transduction; Solid; Transcriptional Regulation; Work; cancer genetics; chromatin remodeling; cofactor; functional genomics; histone acetyltransferase; histone modification; histone-binding proteins; hormonal signals; metabolic abnormality assessment; pyruvate dehydrogenase; recruit; response; senescence; transcription factor; tumor progression

Project Summary Nuclear enzymes, chromatin and its modifications mediate the responses of cells and organisms to dynamic environments. Chromatin remodeling influences gene expression by providing transcription factors and the transcription machinery with dynamic access to an otherwise tightly packaged genome. Many chromatin-modifying enzymes require metabolites as cofactors; therefore, the cell metabolic state can influence chromatin structure and epigenetic processes. However, little is known about how metabolic states and chromatin regulation are coordinated to allow cells and organisms to respond to environmental conditions. We are using Arabidopsis to study the metabolic states and chromatin regulation in ethylene signaling. Our group pioneered the study of histone regulation in ethylene signaling. The ethylene signal is perceived on the endoplasmic reticulum (ER) membrane, and the cleavage and nuclear translocation of EIN2 mediates the signal from the ER membrane to the nucleus. The EIN2 C-terminus (EIN2-C) is cleaved and translocated to the nucleus to initiate the ethylene response. During the last funding period, we discovered that EIN2, an essential signaling factor, is also a key component of the histone modification that directly regulates H3K14Ac and H3K23Ac to mediate the transcriptional response to ethylene. EIN2 is thus the direct link between ethylene signaling and chromatin regulation. We also identified a noncanonical histone acetyltransferase (HAT) domain-containing protein EHAT that directly interacts with the EIN2-C in the nucleus. Our preliminary data strongly suggest that EHAT links EIN2-mediated ethylene signaling with EIN2-meidated regulation of histone acetylation. Strikingly, our new data provide solid evidence that the pyruvate dehydrogenase complex, which converts pyruvate to acetyl-CoA, the acetyl-donor of histone acetylation, interacts with EIN2-C and that the subunits of the complex can translocate from the mitochondria to the nucleus in response to ethylene. This new discovery provides a strong rational for the hypothesis that acetyl-CoA biosynthesis is involved in the EIN2-mediated interplay between chromatin regulation and ethylene signaling. In this proposal, we will study metabolic states and chromatin regulation in ethylene signaling by focusing on the following specific aims: (1) elucidate the detailed mechanisms governing EIN2-dependent histone modification in response to ethylene; (2) investigate the function of the metabolic enzyme pyruvate dehydrogenase in EIN2- mediated chromatin and transcriptional regulation in response to ethylene, and (3) elucidate the molecular mechanisms by which the EIN2-C is translocated to the nucleus in response to ethylene. Our work will have broad implications as dysfunctions of nuclear processes contribute directly to cancer progression and genetic disorders and imbalances between metabolism and chromatin activities can trigger severe metabolic disease.

项目总结