Wnt Signaling and Vertebrate embryogenesis

Wnt 信号传导和脊椎动物胚胎发生

• 批准号: 10077866
• 负责人: Xi He
• 金额: $ 73.41万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077866
• 关键词: Address; Biochemical; Biology; CRISPR screen; Cancer Biology; Cell Communication; Cell Differentiation process; Cell Polarity; Cell Proliferation; Cells; Complex; Congenital Abnormality; Degenerative Disorder; Development; Developmental Biology; Disease; Embryo; Embryonic Development; Family; Health; Homeostasis; Human; Investigation; Malignant Neoplasms; Modeling; Molecular; Pathway interactions; Pattern; Proteomics; Rana; Research; Role; Signal Transduction; Signaling Protein; Tissues; WNT Signaling Pathway; Wnt proteins; Xenopus; experimental study; genome-wide; human disease; insight; receptor; transcriptomics

Project Summary/Abstract The Wnt family of secreted signaling proteins governs embryogenesis and tissue homeostasis. Deregulated Wnt signaling has been implicated in human diseases including birth defects, cancer, and degenerative disorders. Investigation of the mechanism of Wnt signaling has critical significance for developmental and cancer biology and human health, and will uncover new avenues for disease treatment. Over the last two decades, research in my lab has elucidated many key aspects of the Wnt pathway, including the Wnt receptor complex and various molecules and mechanisms that regulate the complex assembly and downstream signaling, and the mechanism by which the Wnt pathway regulates embryonic patterning and cell polarity in Xenopus embryos. In this application I plan to address fundamental questions on the mechanism of Wnt signaling and its role in vertebrate early embryogenesis by employing genome-wide CRISPR/Cas9 screening, proteomic and transcriptomic analyses in combination with classical biochemical and embryological approaches. I believe that experiments outlined in this application will significantly enhance our understanding of Wnt signaling and early vertebrate embryogenesis, and have broad impact to developmental and cancer/disease biology.

项目总结/文摘