Open-top light-sheet microscopy for non-destructive 3D pathology of prostate biopsies

用于前列腺活检非破坏性 3D 病理学的开顶光片显微镜

基本信息

  • 批准号:
    10082154
  • 负责人:
  • 金额:
    $ 39.77万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-09-05 至 2022-08-31
  • 项目状态:
    已结题

项目摘要

Abstract We are proposing a new approach for prostate cancer pathology using 3D open-top light-sheet (OTLS) microscopy and optical clearing. Approximately 20% of patients with an initial negative prostate biopsy procedure are found to have prostate cancer on a subsequent biopsy procedure. Previous studies have shown that examination of 100% of biopsy tissue improved prostate cancer detection rate, but these studies used labor-intensive manual sectioning techniques that are practical for a clinical laboratory. We hypothesize that imaging 100% of prostate biopsy tissue using OTLS microscopy, rather than 1-2% of the biopsy using current pathology technology, can detect occult carcinoma that would have otherwise been missed in a subset of patients. Furthermore, we intend to show that 3D OTLS can be seamlessly integrated into the current clinical laboratory workflow. The specific aims of this proposal are: 1) Validate that our OTLS methods do not interfere with the current pathology practice of formalin-fixed paraffin-embedded (FFPE) tissue sections, hematoxylin and eosin (H&E) histology, and immunohistochemistry (IHC), and 2) To show that 3D pathology can detect occult cancer missed by traditional microscopy, we will compare diagnosis of “pseudo-H&E” 3D OTLS images to traditional H&E pathology. We will use archived FFPE biopsy blocks from patients with prostate cancer who had a negative initial biopsy result. Through the studies in this proposal, we will validate the non-interference of our innovative 3D OTLS methods with standard pathology practice, a necessary prerequisite for adoption within the pathology community and for regulatory considerations. We will also provide preliminary evidence to support the clinical value of 3D pathology. This project addresses the IMAT goal of developing substantially improved cancer detection and risk assessment technologies.
摘要 我们正在提出一种新的前列腺癌病理方法,使用3D开顶式光片 (OTLS)显微镜和光学清除。约20%的患者初始检测结果为阴性 前列腺活检程序在随后的活检中被发现患有前列腺癌 程序。以前的研究表明,100%的活检组织检查有所改善 前列腺癌的发现率,但这些研究使用的是劳动密集型人工切片 适用于临床实验室的技术。我们假设想象100%的 前列腺活检组织使用OTLS显微镜,而不是1-2%的活检使用电流 病理学技术,可以检测到潜在的癌症,否则就会被遗漏在 患者的子集。此外,我们还打算展示3D OTLS可以无缝集成 纳入目前的临床实验室工作流程。本提案的具体目的是:1)验证 我们的OTLS方法不会干扰目前福尔马林固定的病理实践 石蜡包埋(FFPE)组织切片,苏木精伊红(H&E)组织学,以及 免疫组织化学(IHC),以及2)显示3D病理可以发现隐匿性癌 传统显微镜漏诊,我们将比较“伪H&E”3D OTLS图像的诊断 传统的H&E病理学。我们将使用存档的FFPE活体组织块 最初活检结果为阴性的前列腺癌患者。 通过本方案中的研究,我们将验证我们创新的3D 具有标准病理学实践的OTLS方法,这是在 病理界和监管方面的考虑。我们还将提供初步的 支持3D病理学临床价值的证据。该项目旨在实现IMAT的目标 开发显著改进的癌症检测和风险评估技术。

项目成果

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Nicholas Reder其他文献

Nicholas Reder的其他文献

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{{ truncateString('Nicholas Reder', 18)}}的其他基金

3D diagnosis of Hirschsprung disease using open-top light-sheet microscopy
使用开顶光片显微镜对先天性巨结肠进行 3D 诊断
  • 批准号:
    10484508
  • 财政年份:
    2022
  • 资助金额:
    $ 39.77万
  • 项目类别:
3D quantification of NASH-related liver fibrosis using open-top light-sheet microscopy
使用开顶光片显微镜对 NASH 相关肝纤维化进行 3D 定量
  • 批准号:
    10325461
  • 财政年份:
    2021
  • 资助金额:
    $ 39.77万
  • 项目类别:
Same-day Breast Pathology Results using Open-Top Light-Sheet Microscopy for Image-guided Core Needle Biopsies
使用开顶光片显微镜进行图像引导核心针活检的当天乳腺病理学结果
  • 批准号:
    10253425
  • 财政年份:
    2021
  • 资助金额:
    $ 39.77万
  • 项目类别:

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