Purchase of automatic nucleic acid extractor for molecular diagnostics

购买自动核酸提取仪用于分子诊断

• 批准号: 10085539
• 负责人: Carol W. Maddox
• 金额: $ 4.29万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10085539
• 关键词: Purchase; automatic; nucleic; acid; extractor

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference laboratory. UI VDL is a member of the VET-LIRN, and we are one of the labs involved in several VET-LIRN activities including iSeq-sequencing projects of food-borne pathogens, providing isolates for WGS MiSeq sequencing project as a source lab, participation on antimicrobial resistance pilot project. Both conventional and real-time PCR are used to verify bacterial isolates, and whole genome sequencing is also utilized for identification of bacterial isolates. Currently, the UI VDL has aging automatic nucleic acid extractors that are prone to frequent issues. The UI VDL heavily relies on automatic nucleic acid extractors for molecular testing, two of which are currently out of service because of instrument issues; the remaining instruments are about ten years old. Upgrading the current equipment at UI VDL will significantly increase our testing capacity for routine case investigation and surveillance coordinated by Vet- LIRN.

项目摘要 伊利诺伊大学（UI）兽医诊断实验室（VDL）是一个全方位服务，AAVLD 认证，所有物种，参考实验室。UI VDL是VET-LIRN的成员，我们是 其中一个实验室参与了几个VET-LIRN活动，包括iSeq测序项目， 食源性病原体，作为源实验室为WGS MiSeq测序项目提供分离株， 参与抗菌素耐药性试点项目。常规PCR和实时PCR均 用于验证细菌分离株，全基因组测序也用于鉴定 细菌分离物。目前，UI VDL具有老化的自动核酸提取器， 常见的问题。UI VDL在很大程度上依赖于自动核酸提取器进行分子检测。 测试，其中两个目前由于仪器问题而停止服务;其余的 这些乐器大约有十年的历史了。升级UI VDL的现有设备将大大提高 提高我们的检测能力，用于兽医协调的常规病例调查和监测， LIRN。