Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
基本信息
- 批准号:10089447
- 负责人:
- 金额:$ 38.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-02-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:AdhesionsAffectAgonistAmino AcidsAnteriorArginineAspartic AcidAutoimmuneAutoimmune DiseasesBiochemicalBiocompatible MaterialsBurn injuryCell CountCell ProliferationCell SurvivalCell physiologyCellsCharacteristicsChemicalsCholinergic AgonistsCicatrixConjunctival EpitheliumConjunctival PterygiumCorneaCultured CellsDepositionDevelopmentDiseaseEGF geneElectrolytesEnzyme-Linked Immunosorbent AssayEpithelialEpithelial CellsEyeEyelid structureFibroinsFilmGenetic DiseasesGlassGluesGlycineGoalsGoblet CellsGrantGrowthGrowth FactorHumanImageImmunofluorescence MicroscopyInfectionInflammationInflammatoryIonsLocationMUC5AC geneMeasurementMeasuresMechanicsMediator of activation proteinMedicineMembraneMetalsMolecularMucinsNatural regenerationNerve Growth FactorsOcular cicatricial pemphigoidOperative Surgical ProceduresPainPatientsPhenotypePhysiologicalProductionProliferatingPublishingReproducibilitySerotoninSilkSquamous CellStevens-Johnson SyndromeStimulusStressStructure of molecular layer of cerebellar cortexSurfaceSurgical suturesTechniquesTight JunctionsTissuesTitaniumTrachomaTransplantationTraumaUndifferentiatedVisual impairmentWaterbasebiomaterial compatibilitycell growthcell injurycell typeconjunctivacytokinehealinghuman femalehydrophilicityimprovedmalenovelocular surfacephysical propertypreventrelating to nervous systemrepairedresponsescaffoldsexstandard of caretitanium dioxidewater channelwoundwound closurewound healing
项目摘要
Abstract A1-R01 Grant Title: Construction of Conjunctival Equivalents Using Molecular Deposition
Techniques
A robust, functional conjunctiva with goblet, stratified squamous, and undifferentiated cells is critical for a
transparent cornea and a healthy anterior eye. Ocular surface disorders, including, infectious (trachoma),
autoimmune (ocular cicatricial pemphigoid), and genetic diseases, as well as burns and Stevens-Johnson
syndrome, can damage the ocular surface, especially the conjunctiva, by scarring. Although the conjunctiva
has substantial repair potential, these diseases are painful inflammatory diseases and the patients are
desperate for better treatments. Current treatments include use of redundant conjunctiva or amniotic
membrane. Development of novel biocompatible materials is providing improved treatments in the field of
regeneration medicine. For this proposal we will take advantage of the newly developed molecular layer
deposition (MLD) technique to build coatings of bioactive materials. Our overall goal is to develop a
transplantable and functional conjunctival construct consisting of the three types of human conjunctival
epithelial cells (undifferentiated, stratified squamous, and goblet) growing on our novel surface. The surface is
based on an MLD film deposited on a silk fibroin matrix that is easily manipulated for surgical transplantation.
For SA1 we will characterize the phenotype of conjunctival epithelial cells growing on MLD silk construct and
compare it with cells growing on amniotic membrane. For SA2 we will evaluate the function of the three types
of epithelial cells growing on our constructs of MLD silk in response to physiological stimuli of cell function and
compare them with cells growing on amniotic membrane. For SA3 we will measure the repair response to a
mechanical injury of the cells on constructs of MLD silk compared to amniotic membrane. Cell counting,
molecular, and immunofluorescence microscopy techniques will be used to evaluate amount and phenotype of
cell growth. Physical techniques will be used to determine construct strength. Ca2+ imaging, ELISAs for
MUC5AC secretion, and other physiological techniques will be used to determine function of cells on the
construct. Immunofluorescence microscopy and multi-cytokine measurements will be used to evaluate wound
healing. The final product of three types of conjunctival epithelial cells grown on MLD silk will be functional, and
transplantable and excellent for repair of the diseased and damaged conjunctiva.
摘要A1-R01授权标题:利用分子沉积技术构建结膜等效物
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Darlene A Dartt其他文献
Darlene A Dartt的其他文献
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{{ truncateString('Darlene A Dartt', 18)}}的其他基金
Mechanisms underlying mustard gas-induced conjunctival injury and use of lipid mediators as medical countermeasures
芥子气引起的结膜损伤的机制以及脂质介质作为医疗对策的使用
- 批准号:
10882060 - 财政年份:2023
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
10338062 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
10557095 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
9884619 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell Mucin Secretion in Inflammation and Its Resolution
炎症中结膜杯状细胞粘蛋白的分泌及其解决
- 批准号:
9920424 - 财政年份:2019
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8461558 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8274619 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8663916 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Lacrimal Gland Regeneration:Identification and Isolation of Progenitor Cells
泪腺再生:祖细胞的鉴定和分离
- 批准号:
7978066 - 财政年份:2010
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell Mucin Secretion in Inflammation and Its Resolution
炎症中结膜杯状细胞粘蛋白的分泌及其解决
- 批准号:
8990960 - 财政年份:2009
- 资助金额:
$ 38.22万 - 项目类别:
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