Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
基本信息
- 批准号:10089447
- 负责人:
- 金额:$ 38.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-02-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:AdhesionsAffectAgonistAmino AcidsAnteriorArginineAspartic AcidAutoimmuneAutoimmune DiseasesBiochemicalBiocompatible MaterialsBurn injuryCell CountCell ProliferationCell SurvivalCell physiologyCellsCharacteristicsChemicalsCholinergic AgonistsCicatrixConjunctival EpitheliumConjunctival PterygiumCorneaCultured CellsDepositionDevelopmentDiseaseEGF geneElectrolytesEnzyme-Linked Immunosorbent AssayEpithelialEpithelial CellsEyeEyelid structureFibroinsFilmGenetic DiseasesGlassGluesGlycineGoalsGoblet CellsGrantGrowthGrowth FactorHumanImageImmunofluorescence MicroscopyInfectionInflammationInflammatoryIonsLocationMUC5AC geneMeasurementMeasuresMechanicsMediator of activation proteinMedicineMembraneMetalsMolecularMucinsNatural regenerationNerve Growth FactorsOcular cicatricial pemphigoidOperative Surgical ProceduresPainPatientsPhenotypePhysiologicalProductionProliferatingPublishingReproducibilitySerotoninSilkSquamous CellStevens-Johnson SyndromeStimulusStressStructure of molecular layer of cerebellar cortexSurfaceSurgical suturesTechniquesTight JunctionsTissuesTitaniumTrachomaTransplantationTraumaUndifferentiatedVisual impairmentWaterbasebiomaterial compatibilitycell growthcell injurycell typeconjunctivacytokinehealinghuman femalehydrophilicityimprovedmalenovelocular surfacephysical propertypreventrelating to nervous systemrepairedresponsescaffoldsexstandard of caretitanium dioxidewater channelwoundwound closurewound healing
项目摘要
Abstract A1-R01 Grant Title: Construction of Conjunctival Equivalents Using Molecular Deposition
Techniques
A robust, functional conjunctiva with goblet, stratified squamous, and undifferentiated cells is critical for a
transparent cornea and a healthy anterior eye. Ocular surface disorders, including, infectious (trachoma),
autoimmune (ocular cicatricial pemphigoid), and genetic diseases, as well as burns and Stevens-Johnson
syndrome, can damage the ocular surface, especially the conjunctiva, by scarring. Although the conjunctiva
has substantial repair potential, these diseases are painful inflammatory diseases and the patients are
desperate for better treatments. Current treatments include use of redundant conjunctiva or amniotic
membrane. Development of novel biocompatible materials is providing improved treatments in the field of
regeneration medicine. For this proposal we will take advantage of the newly developed molecular layer
deposition (MLD) technique to build coatings of bioactive materials. Our overall goal is to develop a
transplantable and functional conjunctival construct consisting of the three types of human conjunctival
epithelial cells (undifferentiated, stratified squamous, and goblet) growing on our novel surface. The surface is
based on an MLD film deposited on a silk fibroin matrix that is easily manipulated for surgical transplantation.
For SA1 we will characterize the phenotype of conjunctival epithelial cells growing on MLD silk construct and
compare it with cells growing on amniotic membrane. For SA2 we will evaluate the function of the three types
of epithelial cells growing on our constructs of MLD silk in response to physiological stimuli of cell function and
compare them with cells growing on amniotic membrane. For SA3 we will measure the repair response to a
mechanical injury of the cells on constructs of MLD silk compared to amniotic membrane. Cell counting,
molecular, and immunofluorescence microscopy techniques will be used to evaluate amount and phenotype of
cell growth. Physical techniques will be used to determine construct strength. Ca2+ imaging, ELISAs for
MUC5AC secretion, and other physiological techniques will be used to determine function of cells on the
construct. Immunofluorescence microscopy and multi-cytokine measurements will be used to evaluate wound
healing. The final product of three types of conjunctival epithelial cells grown on MLD silk will be functional, and
transplantable and excellent for repair of the diseased and damaged conjunctiva.
摘要A1-R 01资助题目:使用分子沉积构建结膜等效物
技术
具有杯状、复层鳞状和未分化细胞的强健、功能性结膜对于眼结膜炎的发生至关重要。
透明的角膜和健康的前眼。眼表疾病,包括感染性(沙眼),
自身免疫性(眼瘢痕性类天疱疮)和遗传性疾病,以及烧伤和Stevens-Johnson
综合征,可以损害眼表,特别是结膜,通过疤痕。虽然结膜
具有相当大的修复潜力,这些疾病是痛苦的炎症性疾病,
渴望更好的治疗目前的治疗方法包括使用多余的结膜或羊膜
膜的新型生物相容性材料的开发正在提供治疗领域的改进。
再生医学对于这个建议,我们将利用新开发的分子层
沉积(MLD)技术来构建生物活性材料的涂层。我们的总体目标是发展一个
由三种类型的人结膜组成的可移植的功能性结膜构建体
上皮细胞(未分化、复层鳞状和杯状)生长在我们的新表面上。表面
基于沉积在丝素蛋白基质上的MLD膜,其易于操作用于外科移植。
对于SA 1,我们将表征在MLD丝构建体上生长的结膜上皮细胞的表型,
与生长在羊膜上的细胞相比。对于SA 2,我们将评估三种类型的功能
上皮细胞在我们的MLD丝结构上生长,以响应细胞功能的生理刺激,
与生长在羊膜上的细胞进行比较。对于SA 3,我们将测量对
与羊膜相比,MLD丝构建体上细胞的机械损伤。细胞计数,
分子和免疫荧光显微镜技术将用于评估的数量和表型,
细胞生长将使用物理技术确定结构强度。Ca 2+成像,ELISA用于
MUC 5AC分泌和其他生理学技术将用于确定细胞在肿瘤细胞上的功能。
构建体将使用免疫荧光显微镜和多种细胞因子测量来评价伤口
治愈在MLD丝上生长的三种类型的结膜上皮细胞的最终产物将具有功能,
可移植的并且对于患病和受损的结膜的修复是极好的。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Darlene A Dartt其他文献
Darlene A Dartt的其他文献
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{{ truncateString('Darlene A Dartt', 18)}}的其他基金
Mechanisms underlying mustard gas-induced conjunctival injury and use of lipid mediators as medical countermeasures
芥子气引起的结膜损伤的机制以及脂质介质作为医疗对策的使用
- 批准号:
10882060 - 财政年份:2023
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
10338062 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
10557095 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Construction of Conjunctival Equivalents Using Molecular Deposition Techniques
使用分子沉积技术构建结膜等效物
- 批准号:
9884619 - 财政年份:2020
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell Mucin Secretion in Inflammation and Its Resolution
炎症中结膜杯状细胞粘蛋白的分泌及其解决
- 批准号:
9920424 - 财政年份:2019
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8461558 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8663916 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell NLRP3 Inflammasome in Ocular Surface Bacterial Infection
眼表细菌感染中的结膜杯状细胞NLRP3炎症小体
- 批准号:
8274619 - 财政年份:2012
- 资助金额:
$ 38.22万 - 项目类别:
Lacrimal Gland Regeneration:Identification and Isolation of Progenitor Cells
泪腺再生:祖细胞的鉴定和分离
- 批准号:
7978066 - 财政年份:2010
- 资助金额:
$ 38.22万 - 项目类别:
Conjunctival Goblet Cell Mucin Secretion in Inflammation and Its Resolution
炎症中结膜杯状细胞粘蛋白的分泌及其解决
- 批准号:
8990960 - 财政年份:2009
- 资助金额:
$ 38.22万 - 项目类别:
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