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Construction of Conjunctival Equivalents Using Molecular Deposition Techniques

使用分子沉积技术构建结膜等效物

基本信息

批准号：
10089447
负责人：
Darlene A Dartt
金额：
$ 38.22万
依托单位：
SCHEPENS EYE RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2024-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10089447
关键词：
Adhesions Affect Agonist Amino Acids Anterior Arginine Aspartic Acid Autoimmune Autoimmune Diseases Biochemical Biocompatible Materials Burn injury Cell Count Cell Proliferation Cell Survival Cell physiology Cells Characteristics Chemicals Cholinergic Agonists Cicatrix Conjunctival Epithelium Conjunctival Pterygium Cornea Cultured Cells Deposition Development Disease EGF gene Electrolytes Enzyme-Linked Immunosorbent Assay Epithelial Epithelial Cells Eye Eyelid structure Fibroins Film Genetic Diseases Glass Glues Glycine Goals Goblet Cells Grant Growth Growth Factor Human Image Immunofluorescence Microscopy Infection Inflammation Inflammatory Ions Location MUC5AC gene Measurement Measures Mechanics Mediator of activation protein Medicine Membrane Metals Molecular Mucins Natural regeneration Nerve Growth Factors Ocular cicatricial pemphigoid Operative Surgical Procedures Pain Patients Phenotype Physiological Production Proliferating Publishing Reproducibility Serotonin Silk Squamous Cell Stevens-Johnson Syndrome Stimulus Stress Structure of molecular layer of cerebellar cortex Surface Surgical sutures Techniques Tight Junctions Tissues Titanium Trachoma Transplantation Trauma Undifferentiated Visual impairment Water base biomaterial compatibility cell growth cell injury cell type conjunctiva cytokine healing human female hydrophilicity improved male novel ocular surface physical property prevent relating to nervous system repaired response scaffold sex standard of care titanium dioxide water channel wound wound closure wound healing

项目摘要

Abstract A1-R01 Grant Title: Construction of Conjunctival Equivalents Using Molecular Deposition Techniques A robust, functional conjunctiva with goblet, stratified squamous, and undifferentiated cells is critical for a transparent cornea and a healthy anterior eye. Ocular surface disorders, including, infectious (trachoma), autoimmune (ocular cicatricial pemphigoid), and genetic diseases, as well as burns and Stevens-Johnson syndrome, can damage the ocular surface, especially the conjunctiva, by scarring. Although the conjunctiva has substantial repair potential, these diseases are painful inflammatory diseases and the patients are desperate for better treatments. Current treatments include use of redundant conjunctiva or amniotic membrane. Development of novel biocompatible materials is providing improved treatments in the field of regeneration medicine. For this proposal we will take advantage of the newly developed molecular layer deposition (MLD) technique to build coatings of bioactive materials. Our overall goal is to develop a transplantable and functional conjunctival construct consisting of the three types of human conjunctival epithelial cells (undifferentiated, stratified squamous, and goblet) growing on our novel surface. The surface is based on an MLD film deposited on a silk fibroin matrix that is easily manipulated for surgical transplantation. For SA1 we will characterize the phenotype of conjunctival epithelial cells growing on MLD silk construct and compare it with cells growing on amniotic membrane. For SA2 we will evaluate the function of the three types of epithelial cells growing on our constructs of MLD silk in response to physiological stimuli of cell function and compare them with cells growing on amniotic membrane. For SA3 we will measure the repair response to a mechanical injury of the cells on constructs of MLD silk compared to amniotic membrane. Cell counting, molecular, and immunofluorescence microscopy techniques will be used to evaluate amount and phenotype of cell growth. Physical techniques will be used to determine construct strength. Ca2+ imaging, ELISAs for MUC5AC secretion, and other physiological techniques will be used to determine function of cells on the construct. Immunofluorescence microscopy and multi-cytokine measurements will be used to evaluate wound healing. The final product of three types of conjunctival epithelial cells grown on MLD silk will be functional, and transplantable and excellent for repair of the diseased and damaged conjunctiva.

摘要A1-R01授权标题：利用分子沉积技术构建结膜等效物