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The Sensing Liver: Localization and Ligands

感知肝脏：定位和配体

基本信息

批准号：
10093039
负责人：
Blythe D Shepard
金额：
$ 11.7万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10093039
关键词：
Address Attenuated Bile Acids Biological Assay Biological Models Chemoreceptors Coupled Data Diabetes Mellitus Disease Endothelial Cells Energy-Generating Resources Epithelial Cells Esthesia Exhibits Female Funding Funding Agency Future G-Protein-Coupled Receptors GTP-Binding Proteins Gene Chips Gene Expression Glucose Grant Health Hepatic Hepatocyte Heterotrimeric GTP-Binding Proteins Home Homeostasis Human body Immunohistochemistry Kidney Knockout Mice Knowledge Kupffer Cells Ligand Binding Ligands Link Liver Messenger RNA Metabolic Metabolic Diseases Metabolism Mus Opsin Organ Orphan Perception Physiological Physiological Processes Physiology Preparation Proteins Proximal Kidney Tubules Role Second Messenger Systems Sensory Sensory Receptors Signal Transduction Small Intestines Smell Perception Sodium Taste Buds Taste Perception Time Tissues Transmembrane Domain Triglycerides Vision Work Xenobiotics base blood glucose regulation cholangiocyte diabetic glucose tolerance improved in vitro Model interest male novel olfactory receptor receptor receptor function response

项目摘要

Project Summary Sensory receptors are seven transmembrane domain G protein-coupled chemoreceptors (GPCRs) that govern one’s sensation and interpretation of their surrounding world. These receptors, that include olfactory receptors (ORs), taste receptors (TRs), opsins, and other understudied sensory GPCRs (GPRs), are expressed throughout the body where they help to regulate diverse physiological functions. In work funded by a K01 grant (DK106400), we found that one of these receptors, Olfr1393, is expressed in a variety of tissues including the kidney, liver, and small intestine where it contributes to the regulation of glucose reabsorption and the progression of diabetes. To identify additional sensory receptors that may also modulate metabolic function, we screened the liver, the largest metabolic organ in the body, using qPCR gene expression array cards. To date, we have found a total of 86 sensory receptors expressed in murine liver, and have narrowed down the list to 13 ‘highly expressed’ receptors. The aim of this grant is to completely characterize these receptors. To do so, we must understand where they are found (both at the cellular and subcellular level) and identify their ligand profile. The data acquired from this R03 proposal will uncover promising new leads that will assist us in determining the functions that these sensory receptors have in both health and disease.

项目总结