Protecting Pigs From Enzootic Pneumonia: Rational Design Of Safe Attenuated Vaccines.
保护猪免受地方性肺炎:安全减毒疫苗的合理设计。
基本信息
- 批准号:BB/X017540/1
- 负责人:
- 金额:$ 58.65万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2023
- 资助国家:英国
- 起止时间:2023 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Mycoplasma hyopneumonia (M.hyop) is the primary pathogen of enzootic pneumonia (EP), a chronic respiratory disease in pigs. Infections occur worldwide and cause major economic losses to the pig industry, thus having a significant socio-economic impact on pig farmers. Close contact between infected and susceptible pigs is the main route of M.hyop transmission. Piglets are considered free from M.hyop at birth, as in utero transmission has not been documented, and first exposure events occur during the lactation period, when piglets are in contact with dams shedding the microorganism. In fact, the length of the lactation period has been suggested as one risk factor for piglet colonization with M.hyop prior to weaning, and given that the prevalence of M.hyop is around 30%, this places piglets under an enormous risk to be infected. Unfortunately, there are currently no commercial vaccines available that would prevent this initial infection. Indeed, piglet colonization with M.hyop at weaning age is of special importance in segregated production systems, where pigs are transferred to clean facilities for the growing and finishing phases. It has been proposed that the initial group colonisation with M.Hyop determines downstream clinical presentation and disease severity, which is further impacted by the long duration of M.hyop infection that can reach up to 240 days as well as the ability to be infective even after wind dispersal (as far as 9.2km from an infected farm). Thus, an interruption of the colonisation cycle through effective vaccines will benefit the whole porcine production system. M.hyop is primarily found on the mucosal surface of the trachea, bronchi and bronchioles, with different adhesins and lipoproteins being involved in the adherence process, thus rendering infected animals more susceptible to secondary infections. Specifically, the destruction of the mucociliary apparatus, together with the ability of M.hyop to module the immune response, enhances the susceptibility of infected pigs to secondary pathogens. Whereas M.hyop is still susceptible to a variety of antibiotics, their use needs to be reduced to avoid occurrence of multi-drug resistant strains as well as the general drive to reduce antimicrobial usage. Current available vaccines are often cost-efficient but mainly consist of inactivated vaccines which improve production parameters but do not prevent infection or spread of the pathogen, and their use is often limited to one age group, i.e. sows or piglets after 3 weeks of age. Thus, research on developing new vaccines that confer protective immunity and reduce transmission is a priority, as well as optimization of protocols to eliminate M.hyop from pig herds. Here, we are aiming to produce strains of bacteria which are unable to cause disease in pigs but still stimulate a protective immune response. This work builds on previous BBSRC-funded work identifying genes which are essential for survival of the pathogen in pigs and AHDB funded work to isolate strains of bacteria from UK pigs. Once complete, the project will have discovered bacterial genes that are necessary for disease as a basis for the development of better vaccines to prevent, and prevent the circulation of, enzootic pneumonia.
肺炎支原体(m.h oop)是猪的一种慢性呼吸道疾病地方性肺炎(EP)的主要病原体。感染发生在世界各地,给养猪业造成重大经济损失,从而对养猪户产生重大的社会经济影响。感染猪与易感猪之间的密切接触是hyop传播的主要途径。仔猪在出生时被认为没有m.h oop,因为没有在子宫内传播的记录,并且首次暴露事件发生在哺乳期,当仔猪与排出微生物的母鼠接触时。事实上,哺乳期的长短被认为是仔猪在断奶前感染m.h oop的一个危险因素,鉴于m.h oop的患病率约为30%,这使得仔猪面临着巨大的感染风险。不幸的是,目前还没有商业疫苗可以预防这种初始感染。事实上,断奶仔猪在隔离生产系统中与hyop的定植特别重要,在隔离生产系统中,猪被转移到清洁的设施中进行生长和肥育阶段。有人提出,m.h oop最初的群体定殖决定了下游的临床表现和疾病严重程度,这进一步受到m.h oop感染持续时间长(可达240天)以及即使在风传播(距离受感染农场9.2公里)后仍具有传染性的影响。因此,通过有效疫苗中断定植周期将有利于整个猪生产系统。m.h oop主要存在于气管、支气管和细支气管的粘膜表面,不同的黏附素和脂蛋白参与黏附过程,使感染的动物更容易继发感染。具体来说,粘膜纤毛器官的破坏,加上m.h oop调节免疫反应的能力,增强了受感染猪对继发性病原体的易感性。鉴于m.h oop仍然对多种抗生素敏感,因此需要减少抗生素的使用,以避免出现多重耐药菌株,并减少抗菌药物的使用。目前可用的疫苗通常具有成本效益,但主要由灭活疫苗组成,可改善生产参数,但不能预防病原体的感染或传播,而且它们的使用通常仅限于一个年龄组,即3周龄以上的母猪或仔猪。因此,研究开发具有保护性免疫和减少传播的新疫苗,以及优化从猪群中消除猪分枝杆菌的方案是一个优先事项。在这里,我们的目标是生产不能引起猪疾病但仍能刺激保护性免疫反应的细菌菌株。这项工作建立在先前bbsrc资助的工作的基础上,该工作确定了对猪体内病原体存活至关重要的基因,以及AHDB资助的从英国猪中分离细菌菌株的工作。一旦完成,该项目将发现疾病所必需的细菌基因,作为开发更好的疫苗以预防和防止地方性肺炎传播的基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Dirk Werling其他文献
WC1(+) gammadelta T cells indirectly regulate chemokine production during mycobacterium bovis infection in SCID-bo mice.
WC1( ) gammadelta T 细胞在 SCID-bo 小鼠牛分枝杆菌感染期间间接调节趋化因子的产生。
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:4.3
- 作者:
A. Alvarez;J. Endsley;Dirk Werling;D. M. Estes - 通讯作者:
D. M. Estes
Therapeutic targeting of the innate immune system in domestic animals
- DOI:
10.1007/s00441-010-1054-9 - 发表时间:
2010-10-16 - 期刊:
- 影响因子:2.900
- 作者:
Tracey J. Coffey;Dirk Werling - 通讯作者:
Dirk Werling
TITLE: Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis AUTHORS:
标题:牛 SLC11A1 基因遗传变异分析、其对 NRAMP1 表达的影响以及与牛结核病耐药性的潜在关联
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Angela Holder;Rachel Garty;Charlotte Elder;Paula Mesnard;Celine Laquerbe;Marie;M. Salavati;Muhammad Zubair Shabbir;Thomas Tzelos;Timothy Connelly;Bernardo Villarreal;Dirk Werling - 通讯作者:
Dirk Werling
Multi-omics analysis reveals regime shifts in the gastrointestinal ecosystem in chickens following anticoccidial vaccination and emEimeria tenella/em challenge
多组学分析揭示了鸡在抗球虫疫苗接种和柔嫩艾美耳球虫感染后胃肠道生态系统的转变。
- DOI:
10.1128/msystems.00947-24 - 发表时间:
2024-09-17 - 期刊:
- 影响因子:4.600
- 作者:
Po-Yu Liu;Janie Liaw;Francesca Soutter;José Jaramillo Ortiz;Fiona M. Tomley;Dirk Werling;Ozan Gundogdu;Damer P. Blake;Dong Xia - 通讯作者:
Dong Xia
Toll-like receptors in domestic animals
- DOI:
10.1007/s00441-010-1047-8 - 发表时间:
2010-10-07 - 期刊:
- 影响因子:2.900
- 作者:
Thomas W. Jungi;Katja Farhat;Iwan A. Burgener;Dirk Werling - 通讯作者:
Dirk Werling
Dirk Werling的其他文献
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{{ truncateString('Dirk Werling', 18)}}的其他基金
22-ICRAD Call 2 Comparative host and species-specific immune responses of macrophages infected with zoonotic Leptospira interrogans
22-ICRAD Call 2 感染人畜共患问号钩端螺旋体的巨噬细胞的宿主和物种特异性免疫反应比较
- 批准号:
BB/X020061/1 - 财政年份:2023
- 资助金额:
$ 58.65万 - 项目类别:
Research Grant
Establishment of an Organ-on-a-chip facility for Veterinary Species
建立兽医物种器官芯片设施
- 批准号:
BB/X019675/1 - 财政年份:2023
- 资助金额:
$ 58.65万 - 项目类别:
Research Grant
Creating a bovine C-type lectin receptor atlas and identification of their ligands
牛C型凝集素受体图谱的创建及其配体的鉴定
- 批准号:
BB/P008461/1 - 财政年份:2017
- 资助金额:
$ 58.65万 - 项目类别:
Research Grant
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抗菌剂和改进的诊断以实现 CBPP 的综合控制
- 批准号:
BB/H009450/1 - 财政年份:2010
- 资助金额:
$ 58.65万 - 项目类别:
Research Grant
Aetiology pathogenesis and immunology of post-weaning multi-systemic wasting syndrome in pigs: genetic-environmental interactions
猪断奶后多系统消耗综合征的病因、发病机制和免疫学:遗传-环境相互作用
- 批准号:
BB/E018394/1 - 财政年份:2008
- 资助金额:
$ 58.65万 - 项目类别:
Research Grant
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