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VTA VGluT2 Sociability Circuit in Genetic Autism

遗传性自闭症中的 VTA VGluT2 社交回路

基本信息

批准号：
10091988
负责人：
CLIFFORD B SAPER
金额：
$ 43.75万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10091988
关键词：
15q Acute Affect Alleles Back Behavior Disorders Behavioral Binding Bioinformatics Brain Brain Stem Bypass Cell Nucleus Chromosome 15 Complex Copy Number Polymorphism Defect Dopamine Dose Electrophysiology (science)Epilepsy Excitatory Synapse Gene Delivery Gene Dosage Gene Proteins Genes Genetic Genetic Techniques Genetic Transcription Glutamate Receptor Glutamates Human Hypothalamic structure Impairment Intervention Isodicentric Chromosome Learning Ligands Light Link Maps Medial Mediating Methods Midbrain structure Molecular Motivation Mutate Nature Neurons Neuropeptides Nucleus Accumbens Oxytocin Oxytocin Receptor Population Preclinical Testing Reporting Seizures Series Signal Transduction Site Slice Social Behavior Social Interaction Synapses System Techniques Testing Therapeutic Treatment Efficacy UBE3A gene Ventral Tegmental Area Vertebrates Viral Vector Virus autism spectrum disorder base comorbidity delta receptors dosage human model in vivo mouse genetics neuronal circuitry neurophysiology novel optogenetics paraventricular nucleus postsynaptic preclinical study presynaptic programs promoter reconstitution repetitive behavior synergism therapeutic candidate therapeutic evaluation therapeutic target tool transmission process ubiquitin ligase

项目摘要

PROJECT SUMMARY Autism spectrum disorder (ASD) is characterized by its deficits in social interactions. Oxytocin is a hypothalamic neuropeptide known to increase social interactions through its oxytocin receptor (Oxtr) but where this occurs remains undefined. Maternal 15q11-13 triplications (extranumerary isodicentric chromosome 15, idic15) cause a highly penetrant autism that we have linked to the increased dosage of UBE3A. UBE3A encodes an ubiquitin-ligase and transcriptional co-regulator expressed exclusively from the maternal allele in mature neurons. Seizures are a frequent comorbidity in ASD including in idic15. In our recent study (Krishnan et al. Nature 2017), we established that a previously enigmatic population of glutamatergic neurons in the brainstem ventral tegmental area (VTA) drives sociability and found increases of UBE3A and seizures converge to repress expression of autism network gene Cbln1 to impair sociability within these neurons. In Aim 1, we investigate a new concept, that oxytocin receptors mark the specific population of VTA glutamatergic neurons that promote sociability and that oxytocin receptor activity in these neurons is necessary for normal sociability. In Aim 2, we investigate the target site where VTA glutamatergic neurons promote sociability by testing if chemogenetically-controlled activity and Grid1 expression (Cbln1's postsynaptic binding partner that is deleted in autism) in the nucleus accumbens are necessary and sufficient to promote sociability. VTA glutamatergic neurons form excitatory synapses onto nucleus accumbens neurons and we have shown these synapses are impaired by Cbln1 deletion and by seizures. In Aim 3, we test if increases of UBE3A and seizures converge on the specific VTA glutamatergic neurons that express oxytocin receptors to impair sociability and glutamategic transmission if these defects can rescued by adding back Cbln1 to these specialized neurons. In Aim 4, we investigate whether in vivo chemogenetic increases of oxytocin signaling can rescue the VTA glutamatergic neuron to nucleus accumbens synaptic defects and sociability impairments produced by increased UBE3A and seizures. In this study, we combine conditional mouse genetics, stereotaxic viral vector-based gene deliveries methods including VGluT2 and Oxtr promoter intersectional genetics, behavioral chemogenetics, and brain slice optogenetics electrophysiology techniques to uncover a convergent molecular autism gene network and neuronal circuitry where three models of human autism spectrum disorder impair sociability. (1) Increased Ube3a gene dosage (maternal 15q11-13 triplication) and (2) epilepsy convergence to repress Cbln1 in VTA glutamatergic neurons and (3) loss of Grid1, Cbln1's postsynaptic binding partner in nucleus accumbens all impair sociability. We also perform a series of in vivo preclinical tests of the efficacy of therapeutic interventions using viral vector-based methods aimed at these molecules and circuits and the oxytocin system.

项目总结