Purchase of a Q-TOF LC/MS System

购买 Q-TOF LC/MS 系统

• 批准号: 7793911
• 负责人: Michael C Fitzgerald
• 金额: $ 36.88万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-09 至 2011-08-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793911
• 关键词: 16 year old; 7 year old; Aging; Biological Models; Biopolymers; Chemistry; Computer software; Data; Electrospray Ionization; Environment; Faculty; Funding; Funding Applicant; Housing; Information Systems; Liquid Chromatography; Mass Spectrum Analysis; N.I.H. Research Support; Postdoctoral Fellow; Proteomics; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; System; Technology; Training; Universities; Walking; base; graduate student; instrument; ion source; liquid chromatography mass spectrometry; mass spectrometer; meetings; member; operation; public health relevance

DESCRIPTION (provided by applicant): Funds are requested to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The major components of the requested mass spectrometer system (Model Number 6520) include: an accurate mass Q-TOF mass spectrometer equipped with a dual spray electrospray ionization (ESI) ion source, an HPLC-Chip cube mass spectrometry (MS) interface, a nanoflow liquid chromatography (LC) system; and the requisite data system and software to acquire, view, and manipulate mass spectral data. The requested instrument, which will be housed and maintained in the Duke Chemistry Department's Instrument Facility, will not only help meet the general mass spectrometry needs of the eight major users outlined in this proposal, but it will also help meet the general mass spectrometry needs of the Facility's entire user base, which includes 20+ faculty members in the Chemistry Department and 100+ graduate students and post-doctoral researchers in the Chemistry Department, as well as number of researchers from other departments at Duke (many of which are involved in NIH-supported research projects). The proposed instrument will facilitate the analysis of a wide variety of sample types, including small organic molecules as well as both synthetic and naturally occurring biopolymers. The Chemistry Department's need to add a Q-TOF mass spectrometer system to its Instrument Facility is three-fold: (1) it will provide replacement capabilities for an aging workhorse, the 16 year old JEOL SX-102 HRMS system, in the Department's Instrument Facility; (2) it will reduce the backlog of samples/users associated with another of the Department's workhorse mass spectrometers, a 7 year old Agilent IonTrap LC-MS system; and (3) it will bring exciting new proteomics capabilities to the Chemistry Department Instrument Facility, which is the only shared resource on campus to offer appropriately trained users direct walk-up access to its instruments. The reliability, robustness, and ease-of-use afforded by the requested instrument with its HPLC-Chip cube MS interface will greatly facilitate operation of the instrument in the Chemistry Department Facility's open access environment. This will make the instrument a unique resource on the Duke campus. PUBLIC HEALTH RELEVANCE: Funds are requested for the Duke University Chemistry Department Instrument Facility to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The instrument will be uniquely configured to facilitate the characterization of a wide variety of sample types ranging from small organic molecules to large naturally occurring biopolymers. The instrument will be available for direct walk-up use by appropriately trained users of the Duke Chemistry Department's Instrument Facility, many of whom are engaged in NIH-supported research.

描述（由申请人提供）：申请资金用于从Agilent Technologies购买精确质量Q-TOF LC/MS质谱仪系统。所要求的质谱仪系统（型号6520）的主要组件包括：配备双喷雾电喷雾电离（ESI）离子源的精确质量Q-TOF质谱仪、HPLC-Chip立方体质谱（MS）接口、纳流液相色谱（LC）系统;以及采集、查看和操作质谱数据所需的数据系统和软件。所要求的仪器将在杜克化学系的仪器设施中进行安置和维护，不仅有助于满足本提案中概述的八个主要用户的一般质谱分析需求，而且还有助于满足设施整个用户群的一般质谱分析需求，其中包括化学系20多名教师和100多名化学系研究生和博士后研究人员，以及来自杜克其他部门的研究人员（其中许多人参与了NIH支持的研究项目）。拟议的仪器将有助于分析各种各样的样品类型，包括有机小分子以及合成和天然存在的生物聚合物。化学系需要在其仪器设施中增加Q-TOF质谱仪系统，这有三个方面的需要：（1）它将为该系仪器设施中老化的主力设备，即已有16年历史的JEOL SX-102 HRMS系统提供更换能力;（2）它将减少与该部门另一台主力质谱仪有关的样品/用户的积压，一个7岁的安捷伦离子阱LC-MS系统;和（3）它将带来令人兴奋的新的蛋白质组学能力的化学系仪器设施，这是唯一的共享资源，在校园内提供适当的培训用户直接步行访问其仪器。所要求的仪器及其HPLC-Chip cube MS接口的可靠性、鲁棒性和易用性将极大地促进化学系设施开放访问环境中仪器的操作。这将使该仪器成为杜克校园的独特资源。 公共卫生相关性：杜克大学化学系仪器设施申请资金，用于从安捷伦科技公司购买精确质量Q-TOF LC/MS质谱仪系统。该仪器将被独特地配置，以促进各种各样的样品类型的表征，从小的有机分子到大的天然存在的生物聚合物。该仪器将可供杜克化学系仪器设施的经过适当培训的用户直接使用，其中许多人从事NIH支持的研究。