Aerosol Management Platform with Exposure Chamber

描述（由申请人提供）：我们建议从Biaera Technologies购买AeroMP气溶胶管理平台和一个完整的动物暴露室。AeroMP通过全身暴露室或仅鼻InTox暴露系统同时控制多达24只小鼠的气溶胶暴露，该系统已在肺部生物学中心提供。AeroMP先进的计算机化控制系统监测和记录气溶胶生成过程中的气流和压力，允许在实验中重复气溶胶暴露。平行采样端口允许表征被递送到暴露室的气溶胶。AeroMP与全身暴露室和InTox鼻暴露系统的组合将由华盛顿大学肺部生物学中心的广泛研究人员以及相关合作者使用。该系统也将提供给所有研究人员在新的华盛顿大学南湖联合研究校园。全身暴露系统将用于将小鼠暴露于各种BSL 1和2微生物病原体，如流感病毒、小鼠肺炎病毒、铜绿假单胞菌和金黄色葡萄球菌，以促进对宿主防御、肺损伤和免疫力的持续研究。全身暴露系统还将用于输送颗粒气溶胶，如柴油机废气，以研究心肺疾病对颗粒污染暴露的反应机制。InTox鼻暴露系统与AeroMP结合使用，将用于雾化小体积的有价值的试剂，如药理学试剂或含有shRNA的腺病毒载体，以实现有效递送，从而可以解剖与肺部疾病和愈合相关的机制途径。

DESCRIPTION (provided by applicant): We propose to purchase the AeroMP aerosol management platform and a whole animal exposure chamber from Biaera Technologies. The AeroMP controls aerosol exposure for up to 24 mice simultaneously via either the whole body exposure chamber or a nose-only InTox exposure system, which is already available in the Center for Lung Biology. The sophisticated computerized control system of the AeroMP monitors and records airflows and pressures during aerosol generation, allowing reproducible aerosol exposures across experiments. A parallel sampling port allows characterization of the aerosol being delivered to the exposure chamber. The AeroMP combined with both the whole body exposure chamber and the InTox nose-only exposure system will be used by a broad group of investigators within the University of Washington Center for Lung Biology as well as associated collaborators. The system will also be available to all researchers within the new University of Washington South Lake Union Research Campus. The whole body exposure system will be used for exposure of mice to a variety of BSL 1 and 2 microbial pathogens such as influenza, pneumonia virus of the mouse, Pseudomonas aeruginosa, and Staphylococcus aureus to facilitate ongoing investigations into host defense, lung injury, and immunity. The whole body exposure system will also be used to deliver particulate aerosols such as diesel exhaust to study mechanisms of cardiopulmonary disease in response to particulate pollution exposure. The InTox nose-only exposure system coupled with the AeroMP will be used to eaerosolize small volumes of valuable reagents such as pharmacological agents or adenoviral vectors containing shRNA for efficient delivery, allowing dissection of mechanistic pathways involved in lung disease and healing.