Aerosol Management Platform with Exposure Chamber

带暴露室的气溶胶管理平台

• 批准号: 7792792
• 负责人: William A Altemeier
• 金额: $ 11.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-03 至 2011-07-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792792
• 关键词: Adenovirus Vector; Aerosols; Animals; Biology; Coupled; Diesel Exhaust; Dissection; Exposure to; Generations; Healed; Host Defense; Immunity; Influenza; Investigation; Lung; Lung diseases; Monitor; Murine pneumonia virus; Mus; Nose; Particulate; Pathway interactions; Pollution; Pseudomonas aeruginosa; Pulmonary Heart Disease; Reagent; Records; Research; Research Personnel; Sampling; Staphylococcus aureus; System; Technology; Universities; Washington; computerized; healing; lung injury; microbial; pathogen; pressure; research study; response; small hairpin RNA

DESCRIPTION (provided by applicant): We propose to purchase the AeroMP aerosol management platform and a whole animal exposure chamber from Biaera Technologies. The AeroMP controls aerosol exposure for up to 24 mice simultaneously via either the whole body exposure chamber or a nose-only InTox exposure system, which is already available in the Center for Lung Biology. The sophisticated computerized control system of the AeroMP monitors and records airflows and pressures during aerosol generation, allowing reproducible aerosol exposures across experiments. A parallel sampling port allows characterization of the aerosol being delivered to the exposure chamber. The AeroMP combined with both the whole body exposure chamber and the InTox nose-only exposure system will be used by a broad group of investigators within the University of Washington Center for Lung Biology as well as associated collaborators. The system will also be available to all researchers within the new University of Washington South Lake Union Research Campus. The whole body exposure system will be used for exposure of mice to a variety of BSL 1 and 2 microbial pathogens such as influenza, pneumonia virus of the mouse, Pseudomonas aeruginosa, and Staphylococcus aureus to facilitate ongoing investigations into host defense, lung injury, and immunity. The whole body exposure system will also be used to deliver particulate aerosols such as diesel exhaust to study mechanisms of cardiopulmonary disease in response to particulate pollution exposure. The InTox nose-only exposure system coupled with the AeroMP will be used to eaerosolize small volumes of valuable reagents such as pharmacological agents or adenoviral vectors containing shRNA for efficient delivery, allowing dissection of mechanistic pathways involved in lung disease and healing.

描述（由申请人提供）：我们拟向Biaera Technologies购买AeroMP气溶胶管理平台和全动物暴露室。AeroMP可以同时控制多达24只小鼠的气溶胶暴露，通过全身暴露室或仅鼻子暴露的inx暴露系统，该系统已经在肺生物学中心可用。AeroMP复杂的计算机控制系统监测和记录气溶胶产生过程中的气流和压力，允许在实验中重复气溶胶暴露。一个平行采样端口允许表征被输送到暴露室的气溶胶。AeroMP结合了全身暴露室和InTox仅鼻子暴露系统，将被华盛顿大学肺生物学中心的大量研究人员以及相关合作者使用。该系统也将适用于华盛顿大学南湖联合研究新校区的所有研究人员。全身暴露系统将用于小鼠暴露于多种BSL 1和2微生物病原体，如流感、小鼠肺炎病毒、铜绿假单胞菌和金黄色葡萄球菌，以促进正在进行的宿主防御、肺损伤和免疫研究。全身暴露系统还将用于输送柴油尾气等微粒气溶胶，以研究微粒污染暴露后心肺疾病的机制。InTox仅鼻腔暴露系统与AeroMP将用于雾化小体积有价值的试剂，如药理学试剂或含有shRNA的腺病毒载体，以便有效递送，允许解剖涉及肺部疾病和愈合的机制途径。