Ibis T-6,000 Biosensor System

Ibis T-6,000 生物传感器系统

• 批准号: 7842244
• 负责人: CYNTHIA SEARS
• 金额: $ 64.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7842244
• 关键词: Animals; Antimicrobial Resistance; Bacteria; Baltimore; Base Composition; Biosensor; Categories; Cities; Clinical; Colon; Colon Carcinoma; Complex; Computer software; Databases; Detection; Diagnosis; Diarrhea; Diffuse; Disease; Economics; Electrospray Ionization; Employment; Family suidae; Feces; Funding; Genomics; Hospitals; Human; Immunocompromised Host; Individual; Industry; Inflammatory Bowel Diseases; Influenza; Influenza A Virus, H1N1 Subtype; Institution; Interstitial Lung Diseases; Intestinal Diseases; Investigation; Lead; Life; Liver diseases; Lung diseases; Malignant Neoplasms; Malignant neoplasm of prostate; Maryland; Methods; Microbe; Minor; Morbidity - disease rate; Multi-Drug Resistance; N.I.H. Research Support; Occupations; Organism; Positioning Attribute; Prevention therapy; Public Health; Request for Proposals; Research Project Grants; Septicemia; Specimen; Staphylococcus aureus; Structure; System; Time; Tissues; Transplant Recipients; United States; Virulence; Virulent; Virus; falls; fungus; improved; influenzavirus; instrument; microbiome; mortality; novel strategies; pathogen; public health relevance; respiratory; retinal rods; tumor progression

DESCRIPTION (provided by applicant): This proposal requests funds for an Ibis T6000 Biosensor System that incorporates broad-range PCR followed by electrospray ionization time-of-flight (ESI-TOF) mass spectrometric analysis to determine the precise base composition of the PCR amplicons. The Ibis T6000 System then uses a proprietary software package with an extensive genomic database to permit high throughput, rapid and sensitive identification of microbes (bacteria, viruses, fungi) in original, complex human or animal specimens including respiratory secretions and feces. The platform can be customized making it highly adaptable to individual research projects and it is the only microbe discovery method available that permits potential organism identification to the species level. Most recently, this system was used to diagnosis the first swine H1N1 2009 cases in the United States. The instrument will directly benefit 6 major user groups and 9 minor user groups, all of whom have NIH research support. The diverse research projects to be supported by the Ibis T6000 fall into 3 major categories: 1) definition of the structure of the fecal, respiratory or tissue microbiome in inflammatory bowel disease, colon and prostate cancer, progression of liver disease and transplant recipients with rejection and idiopathic lung diseases; 2) detection of specific pathogens (Staphylococcus aureus, nosocomial multidrug resistant gram negative rods, influenza virus), their virulence determinants and antimicrobial resistance determinants in different clinical settings; and 3) identification of disease-causing pathogens in life-threatening, but frequently undiagnosed, illnesses in immunocompromised hosts including diffuse interstitial lung disease, diarrheal disease and septicemia. Every year Johns Hopkins Institutions directly generate about $10 billion in economic activity in the State of Maryland, a 43% increase from the $7 billion generated in 2002 and the equivalent of one of every twenty-four dollars in the state's economy today. In 2008, Johns Hopkins Institutions provided 45,000 jobs and created 700 new jobs each year since 2002. Directly and indirectly Johns Hopkins Institutions support more than 100,000 jobs in Maryland, one of every 29 in the state. In Baltimore City alone Johns Hopkins directly and indirectly supports 60,000 jobs, or 16.7 % of all City employment. This application will create one job immediately and is predicted to yield, through discovery, both retention and new academic and industry/commercial positions. In addition, this acquisition yields eight new positions to manufacture and support the Ibis T6000. PUBLIC HEALTH RELEVANCE: The Ibis T6000 Biosensor System will allow investigations into the contribution of microbes to a wide-range of diseases including respiratory disease in cancer and transplant patients, intestinal diseases such as inflammatory bowel diseases, diarrhea and colon cancer and prostate cancer. The Ibis T6000 Biosensor System will further identify virulent bacteria and viruses such as Staphylococcus aureus, hospital-associated bacteria and influenza that are associated with excess morbidity and mortality. Defining which microbes and how they contribute to these diseases will lead to new approaches to diagnosis, prevention and therapy and make a major contribution to improved public health.

描述（由申请人提供）：该提案要求为Ibis T6000生物传感器系统提供资金，该系统采用宽范围PCR，然后进行电喷雾电离飞行时间（ESI-TOF）质谱分析，以确定PCR扩增子的精确碱基组成。然后，Ibis T6000系统使用专有软件包和广泛的基因组数据库，可以高通量、快速和灵敏地鉴定原始、复杂的人类或动物标本（包括呼吸道分泌物和粪便）中的微生物（细菌、病毒、真菌）。该平台可以定制，使其高度适应个别研究项目，它是唯一的微生物发现方法，允许潜在的生物识别到物种水平。最近，该系统被用于诊断美国首例猪H1N1 2009病例。该仪器将直接受益于6个主要用户组和9个次要用户组，所有这些用户组都得到了NIH的研究支持。Ibis T6000支持的各种研究项目分为3大类：1）定义炎症性肠病、结肠癌和前列腺癌、肝病进展和移植受者排斥反应和特发性肺病中的粪便、呼吸道或组织微生物组的结构; 2）特定病原体的检测（金黄色葡萄球菌、医院内多重耐药革兰氏阴性杆菌、流感病毒），它们在不同临床环境中的毒力决定因素和抗菌药物耐药性决定因素;和3）鉴定免疫受损宿主中危及生命但经常未确诊的疾病中的致病病原体，所述疾病包括弥漫性间质性肺病、肺炎和败血症。每年约翰霍普金斯学院直接在马里兰州产生约100亿美元的经济活动，比2002年产生的70亿美元增加了43%，相当于今天该州经济中的每24美元。2008年，约翰霍普金斯机构提供了45，000个工作岗位，自2002年以来每年创造700个新工作岗位。约翰·霍普金斯大学直接和间接地为马里兰州提供了超过100，000个就业岗位，占该州每29个就业岗位中的一个。仅在巴尔的摩市，约翰霍普金斯就直接和间接地支持了6万个工作岗位，占该市所有就业岗位的16. 7%。该应用程序将立即创建一个工作岗位，并预计将通过发现产生保留和新的学术和工业/商业职位。此外，此次收购产生了八个新的职位来制造和支持Ibis T6000。 公共卫生关系：Ibis T6000生物传感器系统将允许调查微生物对各种疾病的贡献，包括癌症和移植患者的呼吸系统疾病，肠道疾病，如炎症性肠病，腹泻和结肠癌以及前列腺癌。Ibis T6000生物传感器系统将进一步识别致病细菌和病毒，如金黄色葡萄球菌、医院相关细菌和与过度发病率和死亡率相关的流感。确定哪些微生物以及它们如何导致这些疾病将导致诊断，预防和治疗的新方法，并为改善公共卫生做出重大贡献。