DeltaVision OMX 3-D Structured Illumination Microscope

描述（由申请人提供）：包括8名NIH资助的研究人员在内的用户组请求$1,457,908从应用精密有限责任公司购买三维结构照明显微镜（3D-SIM），该显微镜将被安置在MCB/生物科学成像设施学院的部门。3D-SIM是最近开发的少数超分辨率显微镜方法之一，它提供了一个多世纪以来光学显微镜的首次显着分辨率增加。3D- SIM提供了光学显微镜的所有实验优势（例如，易于样品制备，多种成分的分子特异性标记和相对快速的样品吞吐量），同时提供了以前只有电子或x射线显微镜才能获得的分辨率改进，其样品制备和标记条件非常严格。我们打算使用3D-SIM作为MCB部门现有的最先进的数字荧光显微镜和冷冻电子显微镜设备之间的实验桥梁。该仪器将允许在光学显微镜水平上发展良好的研究项目，但需要更高分辨率的空间信息，这是相对缓慢的EM分析的补充途径。我们利用2009年4月15日和16日在应用精密公司的一台原型仪器上收集的初步数据，戏剧性地展示了这种方法的潜力。在本提案中，使用来自三个主要pi的3D-SIM分析样品提供了重要的新的更高分辨率细节，这将加速这些实验室的研究进展，并开辟新的调查路线。拟议仪器的管理将纳入现有的、成功的成像设施管理计划，该计划由一位博士级别的科学家协调，并由一个部门成像委员会指导。MCB/CBS成像设备目前为加州大学戴维斯分校的大量研究人员提供服务（在2007-2008财政年度，19个部门的80名研究人员为2523小时的使用支付了113,906美元），这些现有用户中的许多人会发现3D- SIM很有用。一个如此复杂和维护成本如此高昂的工具需要修改我们在建议中详细说明的现有和有财政偿付能力的管理计划。该计划包括每年向MCB主席支付$25,000的服务合同承付款。

DESCRIPTION (provided by applicant): A user group including eight NIH funded investigators requests $1,457,908 to purchase a Three-Dimensional Structured Illumination Microscope (3D-SIM) from Applied Precision, LLC to be housed in the Department of MCB/College of Biological Sciences Imaging Facility. 3D- SIM is one of a small number of recently developed super-resolution microscopy methods that offer the first significant resolution increases in light microscopy in over a century. 3D- SIM offers all the experimental advantages of light microscopy (e.g., ease of sample preparation, molecule-specific labeling of multiple components and relatively fast sample throughput) while providing resolution improvements previously only available from electron or x-ray microscopy with their very restrictive sample preparation and labeling conditions. We intend to use the 3D-SIM as an experimental bridge between already existing state-of- the-art facilities for digital fluorescence microscopy and cryo-electron microscopy within the MCB Department. This instrument will allow research projects that are well developed at the light microscopy level, but require higher resolution spatial information, a complimentary path to relatively slow EM analysis. We provide dramatic demonstrations of the potential of this approach using preliminary data collected on a prototype instrument at Applied Precision on April 15th and 16th, 2009. Analysis of samples using 3D-SIM from three Major PIs on this proposal provide significant new higher resolution detail that will accelerate the advancement of research in these labs and open up new lines of investigation. Management of the proposed instrument will be integrated into the existing, successful Imaging Facility management scheme coordinated by a PhD-level scientist and guided by a departmental imaging committee. The MCB/CBS Imaging Facility currently serves a very large number of UC-Davis researchers (80 researchers in 19 departments paying $113,906 for 2523 hours of use in fiscal year 2007-2008) and many of these existing users will find the 3D- SIM useful. An instrument of this complexity and maintenance cost requires modification of our existing and fiscally-solvent management schemes which we detail in the proposal. This scheme includes an annual commitment of $25,000 towards the service contract from the MCB Chair.