Nikon High Content Analysis Microscope with PerkinElmer Columbus Image Data Storage and Analysis System for the MCB/CBS Imaging Facility

 描述(由申请人提供)：一个由七名美国国立卫生研究院资助的研究人员组成的用户团体申请591,278.10美元购买尼康高内容筛选(HCS)显微镜以及PerkinElmer Columbus图像数据存储和分析系统，该系统将安装在加州大学戴维斯分校的MCB/生物科学学院成像设备部。对高吞吐量HCS系统的需求是该设施常规获得的高质量图像的自然结果，将使研究人员能够根据其高分辨率结构/空间信息开发和挖掘屏幕。选定的HCS系统是价格适中的“盒式扫描仪”和高端扫描仪之间的一种明智且具有成本效益的折衷方案，前者允许以不足以满足许多模型生物体的分辨率进行高通量采集，后者提供必要的图像分辨率，但购买和维护费用非常昂贵。所要求的HCS显微镜和分析系统非常适合我们的学术设施环境，因为它易于升级，提供功能强大的图像管理和分析软件，具有可访问的用户界面，并且非常灵活，这与更适合制药行业的“BOX”系统形成了鲜明对比。 正如提案中详细说明的那样，高数值孔径的水或油浸泡透镜的可用性是高效使用HCS系统的关键，因为我们的许多用户使用小型生物体，例如萌芽酵母或贾第鞭毛虫，并且需要高空间分辨率的HCS。我们表明，我们可以通过配备所需的Columbus存储和分析软件的商业高端扫描仪来获得这样的结构细节。然而，我们认为这种仪器的操作和维护费用令人望而却步。正如我们用初步数据显示的那样，建议的替代工具使我们能够获得高含量的数据和图像分析，同时在我们共享成像设施的财政限制下舒适地运行。拟议仪器的管理将整合到现有的、成功的成像设备中，该设备由一名全职的博士级别的科学家协调，并由一个以学院为基础的成像委员会进行指导。MCB/CBS成像设备目前为加州大学戴维斯分校的大量研究人员提供服务：自2005年7月以来，来自37个部门的135个实验室的364名科学家支付了超过30,919个小时的仪器时间，产生了超过1,038,684美元的收入，以帮助运营该设备。正如我们对七个主要PI的研究所详细说明的那样，这些现有用户中的许多人会发现拟议的筛选显微镜很有用。

 DESCRIPTION (provided by applicant): A user group of seven NIH funded investigators requests $591,278.10 to purchase a Nikon High Content Screening (HCS) Microscope together with a PerkinElmer Columbus Image Data Storage and Analysis System, which will be housed in the Department of MCB/College of Biological Sciences Imaging Facility at the University of California, Davis. The need for a high throughput HCS system is a natural outgrowth of the high quality images routinely obtained in this facility and will allow researchers to develop and mine screens based on their high- resolution structural/spatial information. The selected HCS system is a sensible and cost- effective compromise between the moderately-priced "scanners in a box", which allow high throughput acquisition at insufficient resolution for many model organisms, and the high-end scanners, which provide the necessary image resolution, but are very expensive to purchase and maintain. The requested HCS microscope and analysis system is very appropriate for our academic facility environment because it is easy to upgrade, provides powerful image management and analysis software with accessible user interfaces, and is very flexible, which is in contrast to the "box" systems, which are better suited for the drug industry. As detailed in the proposal, the availability of water or oil immersion lenses of high numerical aperture is key to productive use of an HCS system as many of our users employ small-sized organisms, such as budding yeast or Giardia, and require high spatial resolution for HCS. We show that we can obtain such structural detail with a commercial high-end scanner equipped with the requested Columbus storage and analysis software. However, we judge the operating and maintenance costs of such an instrument to be prohibitive. As we show with preliminary data, the alternative instrument proposed allows us to obtain high content data and image analysis, while comfortably operating within the fiscal restraints of our shared Imaging Facility. Management of the proposed instrument will be integrated into the existing, successful Imaging Facility, which is coordinated by a full time, PhD-level scientist and guided by a faculty-based imaging committee. The MCB/CBS Imaging Facility currently serves a large number of UC-Davis researchers: since July 2005, 364 scientists from 135 laboratories in 37 departments have paid for more than 30,919 hours of instrument time generating more than $1,038,684 of income to help operate the Facility. As detailed by the research of our seven major PIs, many of these existing users will find the proposed screening microscope useful.