Nikon High Content Analysis Microscope with PerkinElmer Columbus Image Data Storage and Analysis System for the MCB/CBS Imaging Facility

 描述（由申请人提供）：一个由七名NIH资助的研究者组成的用户组要求支付591，278.10美元，以购买一台Nikon高含量筛选（HCS）显微镜以及一台PerkinElmer哥伦布图像数据存储和分析系统，该系统将安装在戴维斯加州大学MCB/生物科学学院成像设施系。对高通量HCS系统的需求是在该设施中常规获得的高质量图像的自然结果，并且将允许研究人员基于其高分辨率结构/空间信息开发和开采屏幕。所选择的HCS系统是在价格适中的“盒中扫描仪”和高端扫描仪之间的合理且成本有效的折衷，所述“盒中扫描仪”允许在对于许多模式生物体而言分辨率不足的情况下进行高通量采集，所述高端扫描仪提供必要的图像分辨率，但是购买和维护非常昂贵。所要求的HCS显微镜和分析系统非常适合我们的学术设施环境，因为它易于升级，提供功能强大的图像管理和分析软件，具有可访问的用户界面，并且非常灵活，这与更适合制药行业的“盒子”系统形成鲜明对比。 正如提案中所详述的，高数值孔径的水或油浸没透镜的可用性是HCS系统高效使用的关键，因为我们的许多用户使用小型生物体，如芽殖酵母或贾第虫，并且需要HCS的高空间分辨率。我们表明，我们可以获得这样的结构细节与商业高端扫描仪配备所需的哥伦布存储和分析软件。然而，我们认为，这种仪器的运行和维护费用过高。正如我们所展示的初步数据，提出的替代工具使我们能够获得高内容的数据和图像分析，同时在我们共享的成像设施的财政限制内舒适地运行。拟议的仪器的管理将被集成到现有的，成功的成像设施，这是由一个全职的，博士级的科学家协调和指导学院为基础的成像委员会。MCB/CBS成像设施目前为大量的加州大学戴维斯分校研究人员提供服务：自2005年7月以来，来自37个部门135个实验室的364名科学家支付了超过30，919小时的仪器时间，产生了超过1，038，684美元的收入，以帮助运营该设施。正如我们对七个主要PI的研究所详述的那样，这些现有用户中的许多人会发现拟议的筛选显微镜很有用。

 DESCRIPTION (provided by applicant): A user group of seven NIH funded investigators requests $591,278.10 to purchase a Nikon High Content Screening (HCS) Microscope together with a PerkinElmer Columbus Image Data Storage and Analysis System, which will be housed in the Department of MCB/College of Biological Sciences Imaging Facility at the University of California, Davis. The need for a high throughput HCS system is a natural outgrowth of the high quality images routinely obtained in this facility and will allow researchers to develop and mine screens based on their high- resolution structural/spatial information. The selected HCS system is a sensible and cost- effective compromise between the moderately-priced "scanners in a box", which allow high throughput acquisition at insufficient resolution for many model organisms, and the high-end scanners, which provide the necessary image resolution, but are very expensive to purchase and maintain. The requested HCS microscope and analysis system is very appropriate for our academic facility environment because it is easy to upgrade, provides powerful image management and analysis software with accessible user interfaces, and is very flexible, which is in contrast to the "box" systems, which are better suited for the drug industry. As detailed in the proposal, the availability of water or oil immersion lenses of high numerical aperture is key to productive use of an HCS system as many of our users employ small-sized organisms, such as budding yeast or Giardia, and require high spatial resolution for HCS. We show that we can obtain such structural detail with a commercial high-end scanner equipped with the requested Columbus storage and analysis software. However, we judge the operating and maintenance costs of such an instrument to be prohibitive. As we show with preliminary data, the alternative instrument proposed allows us to obtain high content data and image analysis, while comfortably operating within the fiscal restraints of our shared Imaging Facility. Management of the proposed instrument will be integrated into the existing, successful Imaging Facility, which is coordinated by a full time, PhD-level scientist and guided by a faculty-based imaging committee. The MCB/CBS Imaging Facility currently serves a large number of UC-Davis researchers: since July 2005, 364 scientists from 135 laboratories in 37 departments have paid for more than 30,919 hours of instrument time generating more than $1,038,684 of income to help operate the Facility. As detailed by the research of our seven major PIs, many of these existing users will find the proposed screening microscope useful.