Genome Sequencer FLX

基因组测序仪 FLX

• 批准号: 7841366
• 负责人: JOHN J. NGAI
• 金额: $ 62.29万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7841366
• 关键词: Characteristics; DNA; DNA Sequence; Disease; Expressed Sequence Tags; Funding; Gene Expression Profile; Genetic; Genome; Genome Mappings; Human Biology; Human Development; Investigation; Ions; Length; Malignant Neoplasms; Mammalian Cell; Modeling; Multigene Family; Nutritional; Organism; Pattern; Perception; Predisposition; RNA Splicing; Reading; Research Personnel; Science; Sensory; Technology; Toxic Environmental Substances; genetic analysis; instrumentation; mRNA Precursor; novel

DESCRIPTION (provided by applicant): The 454/Roche GS-FLX pyrosequencer will be used for high-throughput DNA sequencing in applications demanding long reads (>400 nt) at high accuracy. The technology and characteristics of this sequencing technology is markedly different than for sequencers such as the Solexa/Illumina Genome Sequencer, which provide short (<75 nt) read lengths with a higher error rate. The ability to obtain long read lengths with high accuracy affords several critical advantages for a number of applications, including (1) the sequencing of novel genomes, (2) EST and transcriptome characterization in organisms lacking a sequenced or well-mapped genome, and (3) the analysis of alternative pre-mRNA splicing. The 454/Roche long-read sequencing technology will be applied to a diverse array of NIH-funded projects as well as other projects of high relevance to biomedical science, including the characterization of multigene families involved in sensory perception; the elucidation of the patterns and mechanisms of alternative pre-mRNA splicing; the characterization of genomes of genetic and evolutionary models of human development and disease; an analysis of the genetic loci associated with cancer; and an investigation of the susceptibility of mammalian cells to nutritional ions and environmental toxins. There are currently no 454 DNA sequencers on the UC Berkeley campus; acquisition of this instrumentation by the FGL will support researchers pursuing these projects, which promise to illuminate the fundamental mechanisms underlying human biology and disease.

描述（由申请人提供）：454/Roche GS-FLX 焦磷酸测序仪将用于需要高精度长读数（>400 nt）的应用中的高通量 DNA 测序。这种测序技术的技术和特点与 Solexa/Illumina 基因组测序仪等测序仪明显不同，后者提供短（<75 nt）读长，但错误率较高。以高精度获得长读长的能力为许多应用提供了几个关键优势，包括（1）新基因组的测序，（2）缺乏测序或良好图谱基因组的生物体中的EST和转录组表征，以及（3）替代前mRNA剪接的分析。 454/罗氏长读长测序技术将应用于 NIH 资助的各种项目以及与生物医学科学高度相关的其他项目，包括与感官知觉有关的多基因家族的表征；阐明选择性前 mRNA 剪接的模式和机制；人类发育和疾病的遗传和进化模型的基因组特征；与癌症相关的遗传位点分析；以及哺乳动物细胞对营养离子和环境毒素的敏感性的研究。目前加州大学伯克利分校校园内没有 454 台 DNA 测序仪； FGL 收购该仪器将支持研究人员开展这些项目，这些项目有望阐明人类生物学和疾病的基本机制。

项目成果

Synthetic and Evolutionary Construction of a Chlorate-Reducing Shewanella oneidensis MR-1.

• DOI: 10.1128/mbio.00282-15
• 发表时间: 2015-05-19
• 期刊: mBio
• 影响因子: 6.4
• 作者: Clark IC;Melnyk RA;Youngblut MD;Carlson HK;Iavarone AT;Coates JD
• 通讯作者: Coates JD

BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction.

• DOI: 10.3389/fpls.2015.00366
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Townsley BT;Covington MF;Ichihashi Y;Zumstein K;Sinha NR
• 通讯作者: Sinha NR

The complete mitochondrial genome of the dusky-footed woodrat(Neotoma fuscipes) (Rodentia, Cricetidae).

• DOI: 10.1080/23802359.2016.1233464
• 发表时间: 2016-10-03
• 期刊: Mitochondrial DNA. Part B, Resources
• 作者: Brown SK;Blois JL
• 通讯作者: Blois JL

Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals.

• DOI: 10.1016/j.molcel.2016.05.032
• 发表时间: 2016-07-07
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Sanz LA;Hartono SR;Lim YW;Steyaert S;Rajpurkar A;Ginno PA;Xu X;Chédin F
• 通讯作者: Chédin F

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain.

• DOI: 10.1371/journal.pbio.2001882
• 发表时间: 2017-03
• 期刊: PLoS biology
• 影响因子: 9.8
• 作者: Lintner NG;McClure KF;Petersen D;Londregan AT;Piotrowski DW;Wei L;Xiao J;Bolt M;Loria PM;Maguire B;Geoghegan KF;Huang A;Rolph T;Liras S;Doudna JA;Dullea RG;Cate JH
• 通讯作者: Cate JH