Multi-Laser Flow Cytometer for Polychromatic Analysis

用于多色分析的多激光流式细胞仪

• 批准号: 7796518
• 负责人: James E Crowe
• 金额: $ 48.25万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-04 至 2011-03-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796518
• 关键词: Arts; Calibration; Cell physiology; Cells; Communities; Complex; Custom; Cytometry; Development; Disease; Fees; Flow Cytometry; Funding; Hour; Immune response; Immune system; Institution; Intervention; Laboratories; Lasers; Maintenance; Malignant Neoplasms; Microscopy; Nature; Quantum Dots; Research; Research Infrastructure; Research Personnel; Resource Sharing; Scientist; Services; Site; Sorting - Cell Movement; Stem cells; Time; Training; Training Support; Universities; Vaccines; Variant; Work; fluorophore; human disease; improved; instrument; instrumentation; medical schools; meetings; novel; operation; public health relevance; repaired; response

DESCRIPTION (provided by applicant): We request funds to purchase a Becton Dickinson custom multilaser LSRII flow cytometer for polychromatic analytical flow cytometry. The current facilities at Vanderbilt University provide a strong infrastructure for conduct of highly technical flow cytometric work, but do not currently meet the scientific needs for the NIH-funded community of scientists. First, many of the scientists in the user group have expanded their use of new fluorophores, quantum dots, and variant GFP molecules in an effort to intensify the polychromatic nature of the work, which is needed for complex functional readouts. Many of these investigators are using these novel combinations of fluorophores in microscopy, but now need the more quantitative analysis that is afforded by flow cyto- metry. The currently available instruments are lacking lasers in critical regions of the spectrum for the conduct of these studies. In addition, researchers are becoming limited in access to analytical instruments because the amount of use of the current instruments exceeds 45 hours per week per instrument, not counting the time needed for daily setup and calibration. Limitation of access has begun to constrain the progress of our NIH-funded researchers. This instrument will support a highly collaborative group of 19 major users from 9 research groups in the School of Medicine. Ongoing, NIH-funded projects by major users that will benefit include research on vaccine responses, cancer, autoimmunse diseases, development, basic immune responses and others. For each project, the requested instrumentation will have an immediate and substantial impact by providing unique new analytical capabilities of the cells under study, and increased access to analytical cytometry. Acquisition and operation of this cytometer by the Vanderbilt Flow Cytometry Core Laboratory, an institutional shared resource, will guarantee user training/support, 24 hour/day 7 day/week access, and instrument maintenance and repair. User fees will cover staff support, service and repair. The institution has made an exceptionally strong financial commitment to support maintenance and use of the instrument. Other researchers will be able to use available time, as the instrument will be sited in an institutional core, thus maximizing use and impact. This Core currently supports over 200 users who we have trained in analytical and sorting cytometry at Vanderbilt. PUBLIC HEALTH RELEVANCE: The proposed state-of-the-art instrument will give researchers a new capability and expanded access to study important functions of cells including the immune system, stem cells, and cell leading to cancer, thereby improving our ability to develop new targets for intervention in human diseases.

描述(由申请者提供)：我们申请资金购买Becton Dickinson定制的多激光LSRII流式细胞仪，用于多色分析流式细胞术。范德比尔特大学目前的设施为开展高技术的流式细胞仪工作提供了强大的基础设施，但目前还不能满足NIH资助的科学家群体的科学需求。首先，用户组中的许多科学家已经扩大了他们对新的荧光团、量子点和不同的GFP分子的使用，以努力加强工作的多色性，这是复杂功能读数所需的。这些研究人员中的许多人在显微镜下使用这些新的荧光团组合，但现在需要更多的定量分析，这是由流动细胞计量学提供的。目前可用的仪器在进行这些研究的光谱的关键区域缺乏激光。此外，研究人员获得分析仪器的机会越来越有限，因为目前的仪器使用量超过每台仪器每周45小时，这还不包括日常设置和校准所需的时间。获取机会的限制已经开始限制我们NIH资助的研究人员的进展。该仪器将支持来自医学院9个研究小组的19个主要用户的高度协作小组。主要用户正在进行的由NIH资助的项目将受益，包括疫苗反应、癌症、自身免疫性疾病、发育、基本免疫反应等方面的研究。对于每个项目，所要求的仪器将通过提供被研究细胞独特的新的分析能力和增加获得分析细胞学的机会而立即产生实质性的影响。范德比尔特流式细胞仪核心实验室是一个机构共享的资源，购买和操作这台细胞仪将保证用户培训/支持、24小时/7天/周访问以及仪器维护和维修。使用费将包括员工支持、服务和维修费用。该机构作出了特别强有力的财政承诺，支持维护和使用该文书。其他研究人员将能够利用可用的时间，因为该工具将设在机构核心，从而最大限度地利用和影响。该核心目前支持200多名用户，他们是我们在Vanderbilt接受过分析和分类细胞术培训的用户。 与公共健康相关：拟议的最先进的仪器将为研究人员提供新的能力和更多的机会来研究细胞的重要功能，包括免疫系统、干细胞和导致癌症的细胞，从而提高我们开发干预人类疾病的新靶点的能力。