Moor Vascular Assessment System -

Moor 血管评估系统 -

• 批准号: 7797862
• 负责人: Brian H. Johnstone
• 金额: $ 13.04万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7797862
• 关键词: Angiogenesis Inhibitors; Animal Model; Area; Arts; Biological; Biological Models; Biology; Blood; Blood Vessels; Blood flow; Bone Marrow; Cells; Communities; Data; Disease; Evaluation; Funding; Future; Generations; Homeostasis; Image; Imagery; Imaging Device; Inferior; Intervention; Laboratories; Lasers; Mediating; Modality; Molecular; Perfusion; Population; Preparation; Process; Research; Research Personnel; Resolution; Scanning; Specimen; Stem cells; System; Technology; Time; Tissues; Vascular Diseases; Vascular remodeling; Work; angiogenesis; attenuation; instrument; interest; neovascularization; progenitor; public health relevance; repaired; response; stem; tumor; vascular bed; vasculogenesis

DESCRIPTION (provided by applicant): This proposal is for the purchase of a Moor Instruments Vascular Assessment System (VAS), which will serve 7 NIH-funded research groups. The VAS is an integrated laser Doppler imaging (LDI) device that will replace our first generation Moor LDI system, which has been working at near maximum capacity for 8 years, but is outdated and produces data of inferior quality due to the low resolution images generated. An additional limitation of the present system is the wavelength of the laser does not penetrate past the most superficial tissue layers and, therefore, does not adequately interrogate the areas of interest. Finally, the present modality does not allow real-time capture of images for determining transient changes in blood flow due to interventions. The demand for LDI on our campus continues to grow, and the proposed system will fulfill the needs of a large number of researchers by providing excellent resolution of vascular beds, allowing assessment of deep tissue microcirculatory systems and providing serial, non-invasive dynamic visualization and quantitation of blood flow, so that new applications for LDI can be accommodated or developed. The VAS can image specimens with very high resolution (less than 100 mm), penetrate up to 3 mm in depth and can capture up to 25 frames per second of video. A typical scan takes only about 15 minutes (including preparation of animal model), and the system comes with an analysis workstation to allow rapid quantitation. The enhanced capabilities of the system will better serve the needs of our vascular biology research community. Applications for the proposed facility include: 1) Assessment of stem cell-induced revascularization of ischemic tissues. 2) Stem and progenitor cell-mediated vasculogenesis in tissue and cell grafts. 3) Serial evaluation of tumor perfusion in response to anti-angiogenic agents 4) Involvement of a bone marrow-derived cell population in neovascularization. 5) Evaluate the contribution of different endothelial progenitor types in vascular remodeling and angiogenesis. 6) Determination of the molecular mechanisms that contribute to disease-associated attenuation of endothelial repair processes and vasoreactivity. The acquisition of the VAS is essential for continued progress on these and future projects requiring this state-of-the-art technology. PUBLIC HEALTH RELEVANCE: This proposal is for purchase of a new laser Doppler imaging system, which will be used to study biological specimens from several laboratories. This technology will make possible new discoveries in the areas of vascular disease, blood homeostasis, assess the activity of compounds that alter blood flow, vascular remodeling and arteriogenesis in normal and diseased tissues.

描述（由申请人提供）：本提案用于购买摩尔仪器血管评估系统（VAS），该系统将为7个NIH资助的研究小组提供服务。VAS是一种集成的激光多普勒成像（LDI）设备，将取代我们的第一代摩尔LDI系统，该系统已在接近最大容量的情况下工作了8年，但由于生成的图像分辨率较低，已经过时并产生质量较差的数据。本系统的另一个限制是激光的波长不能穿透最表层的组织层，因此不能充分地询问感兴趣的区域。最后，目前的模式不允许实时捕获图像以确定由于干预引起的血流的瞬时变化。 我们校园对LDI的需求持续增长，拟议的系统将满足大量研究人员的需求，提供出色的血管床分辨率，允许评估深部组织微循环系统，并提供连续的，非侵入性的动态可视化和定量血流，以便可以容纳或开发LDI的新应用。VAS可以以非常高的分辨率（小于100 mm）对样本进行成像，穿透深度高达3 mm，并且可以捕获高达每秒25帧的视频。一次典型的扫描仅需约15分钟（包括动物模型的准备），系统配有分析工作站，可以快速定量。该系统的增强功能将更好地满足我们血管生物学研究界的需求。拟议设施的应用包括：1）评估干细胞诱导的缺血组织血运重建。2)组织和细胞移植物中干细胞和祖细胞介导的血管发生。3)对抗血管生成剂的肿瘤灌注的系列评价4）骨髓来源的细胞群参与新血管形成。5)评价不同内皮祖细胞类型在血管重塑和血管生成中的作用。6)确定有助于内皮修复过程和血管反应性的疾病相关衰减的分子机制。 收购VAS对于这些和未来需要这种最先进技术的项目的持续进展至关重要。 公共卫生相关性：这项建议是购买一套新的激光多普勒成像系统，用于研究几个实验室的生物标本。这项技术将使血管疾病，血液稳态领域的新发现成为可能，评估改变正常和患病组织中血流，血管重塑和动脉生成的化合物的活性。