Moor Vascular Assessment System -

描述（由申请人提供）：本提案是购买摩尔仪器血管评估系统（VAS），该系统将为7个nih资助的研究小组提供服务。VAS是一种集成的激光多普勒成像（LDI）设备，将取代我们的第一代Moor LDI系统，该系统已经在接近最大容量的情况下工作了8年，但已经过时，并且由于生成的图像分辨率低而产生的数据质量较差。当前系统的另一个限制是激光的波长不能穿透最浅层的组织层，因此不能充分地询问感兴趣的区域。最后，目前的模式不允许实时捕获图像，以确定由于干预引起的血流的瞬态变化。我们的校园对LDI的需求持续增长，提出的系统将满足大量研究人员的需求，通过提供血管床的高分辨率，允许评估深层组织微循环系统，并提供连续的，非侵入性的动态可视化和血流定量，因此LDI的新应用可以适应或开发。VAS可以以非常高的分辨率（小于100毫米）对标本进行成像，穿透深度可达3毫米，并且可以捕获高达每秒25帧的视频。一个典型的扫描只需要大约15分钟（包括动物模型的准备），系统配备了一个分析工作站，可以快速定量。该系统的增强功能将更好地满足我们血管生物学研究界的需求。该设备的应用包括：1)评估干细胞诱导的缺血组织血运重建。2)干细胞和祖细胞介导的组织和细胞移植物血管发生。3)肿瘤灌注对抗血管生成药物反应的系列评价4)骨髓源性细胞群在新生血管形成中的参与。5)评价不同内皮祖细胞类型在血管重构和血管生成中的作用。6)确定与疾病相关的内皮修复过程和血管反应性衰减有关的分子机制。VAS的获得对于这些和未来需要这种最先进技术的项目的持续进展至关重要。

DESCRIPTION (provided by applicant): This proposal is for the purchase of a Moor Instruments Vascular Assessment System (VAS), which will serve 7 NIH-funded research groups. The VAS is an integrated laser Doppler imaging (LDI) device that will replace our first generation Moor LDI system, which has been working at near maximum capacity for 8 years, but is outdated and produces data of inferior quality due to the low resolution images generated. An additional limitation of the present system is the wavelength of the laser does not penetrate past the most superficial tissue layers and, therefore, does not adequately interrogate the areas of interest. Finally, the present modality does not allow real-time capture of images for determining transient changes in blood flow due to interventions. The demand for LDI on our campus continues to grow, and the proposed system will fulfill the needs of a large number of researchers by providing excellent resolution of vascular beds, allowing assessment of deep tissue microcirculatory systems and providing serial, non-invasive dynamic visualization and quantitation of blood flow, so that new applications for LDI can be accommodated or developed. The VAS can image specimens with very high resolution (less than 100 mm), penetrate up to 3 mm in depth and can capture up to 25 frames per second of video. A typical scan takes only about 15 minutes (including preparation of animal model), and the system comes with an analysis workstation to allow rapid quantitation. The enhanced capabilities of the system will better serve the needs of our vascular biology research community. Applications for the proposed facility include: 1) Assessment of stem cell-induced revascularization of ischemic tissues. 2) Stem and progenitor cell-mediated vasculogenesis in tissue and cell grafts. 3) Serial evaluation of tumor perfusion in response to anti-angiogenic agents 4) Involvement of a bone marrow-derived cell population in neovascularization. 5) Evaluate the contribution of different endothelial progenitor types in vascular remodeling and angiogenesis. 6) Determination of the molecular mechanisms that contribute to disease-associated attenuation of endothelial repair processes and vasoreactivity. The acquisition of the VAS is essential for continued progress on these and future projects requiring this state-of-the-art technology. PUBLIC HEALTH RELEVANCE: This proposal is for purchase of a new laser Doppler imaging system, which will be used to study biological specimens from several laboratories. This technology will make possible new discoveries in the areas of vascular disease, blood homeostasis, assess the activity of compounds that alter blood flow, vascular remodeling and arteriogenesis in normal and diseased tissues.