Validation of a Stroke Therapy Comprised of Synergistic Stem Cell-Derived Factors

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 DESCRIPTION (provided by applicant): Stroke is the leading cause of disability in adults in the Western world. Its socioeconomic impact is staggering. Most stroke patients currently have no treatment option given that the only FDA-approved stroke therapy, intravenous tPA for treatment of thrombolysis, must be administered within 5 hours of stroke onset and only about 5% of stroke patients present for treatment within that time window. The development of a therapy with a longer treatment window could benefit the other 95% of patients not currently served. Mesenchymal stem cell (MSC) therapies have shown promise in cerebrovascular disease models; however, their mechanism of action is predominantly through paracrine support of endogenous repair mechanisms rather than direct tissue replacement. We and others have demonstrated the therapeutic potential of cell-free conditioned medium derived from adult MSCs isolated from adipose tissues. Importantly, we have observed morphological and functional benefits when our therapy, NFx-101, is delivered up to 36 hours following onset of ischemia. We propose to demonstrate the efficacy of NFx-101, a partially purified adipose stem cell-derived conditioned medium, when delivered between 6 and 24 hours after ischemia in a clinically relevant, partially humanized mouse embolic stroke model. Mice will be evaluated for morphological and functional changes as a result of treatment. If successful, NFx-101 could become a breakthrough therapy for stroke victims.