Bioinflammation Core

生物炎症核心

• 批准号: 10094228
• 负责人: Jennifer C Sullivan
• 金额: $ 29.19万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10094228
• 关键词: Address; Animal Model; Applications Grants; Biological Assay; Blinded; Blood specimen; Cells; Chronic Kidney Failure; Clinical; Collection; Development; Ensure; Experimental Animal Model; Goals; HMGB1 Protein; Heart failure; Hypertension; IL17 gene; IL6 gene; Immune; Individual; Innate Immune Response; Interleukin-10; Investigation; Maintenance; Measurement; Measures; Methods; Mitochondrial DNA; Molecular; Myocardial Infarction; Pathogenesis; Pattern; Pattern recognition receptor; Peripheral arterial disease; Porcine Reproductive and Respiratory Syndrome; Preparation; Program Research Project Grants; Quality Control; Reagent; Records; Recovery; Reproducibility; Research Personnel; Risk; Role; Running; Sampling; Services; Specimen; Stroke; Structure; TLR4 gene; Tissue Sample; central database; cost; cytokine; experimental analysis; immune activation; novel; programs; success; synergism

BIOINFLAMMATION CORE SUMMARY CC, ERGUL The projects in this Program Project Grant application focus on two key damage associated molecular patterns (DAMPs), high mobility group box 1 (HMGB1) and mitochondrial (mt) DNA, activating the innate immune response via their respective pattern recognition receptors (PPRs) TLR4 and TLR9 resulting in stimulation of a number of cytokines in experimental animal models of hypertension. The Bioinflammation Core (Core C) is structured to serve all three projects with centralized measurements of DAMPs, PRRs, immune cells and downstream cytokines to facilitate the success of the individual projects and achieve the overall goals of this Program Project. The secondary goal is to provide necessary services to enhance the synergy among projects. Core C will be directed by Dr. Ergul and supported by Dr. Babak Baban. Both investigators have a long- standing record of analyzing experimental and clinical specimens with a highly qualified staff. A centralized Core is essential to the success of each project and the Program Project as a whole and offers the following benefits: 1. The coordination of sample preparation within the Bioinflammation Core C will benefit all Projects as Core C will optimize sample recovery and increase efficiency and rigor by allowing analysis of more samples in one run while providing blinded analyses of the samples. 2. Common approach (in many cases identical methods and reagents) to measure the same markers across the projects will provide synergy, quality control and consistency among the projects. 3. The measurement of primary and secondary DAMPs/PRRS in all Projects will cross cultivate the Projects and allow the development of new venues of investigation that otherwise would not have been possible with regular R01 applications. 4. Higher numbers of measurements will be completed at a relatively lower cost with highly skilled research personnel. 5. A close interaction with the Animal Models Core (Core B) will ensure proper collection and storage of specimens and increase reproducibility.

生物炎症核心摘要CC，Ergul 该计划项目授予申请中的项目重点关注的两个关键损害相关的分子模式 （潮湿），高移动性组框1（HMGB1）和线粒体（MT）DNA，激活先天免疫 通过各自的模式识别受体（PPRS）TLR4和TLR9的反应，导致刺激 高血压实验动物模型中的细胞因子数量。生物炎症核心（核心C）是 结构旨在为所有三个项目提供用于潮湿，PRR，免疫细胞和 下游细胞因子促进单个项目的成功并实现总体目标 计划项目。次要目标是提供必要的服务来增强项目之间的协同作用。 C核心将由Ergul博士指导，并由Babak Baban博士提供支持。两位调查人员的长期 与高素质的员工一起分析实验和临床标本的常规记录。集中式 核心对于每个项目的成功和整个程序项目至关重要，并提供以下内容 好处： 1。生物炎症核心C中样本制备的协调将使所有项目受益 核心C将通过允许更多分析来优化样本恢复，并提高效率和严格 样本一跑，同时提供对样品的盲目分析。 2。常见方法（在许多情况下相同的方法和试剂）测量相同标记 在整个项目中，这些项目将提供协同，质量控制和一致性。 3。在所有项目中的原发性和次要潮湿/PRR的测量都将跨越种植 项目并允许开发新的调查场所 使用常规R01应用程序可能。 4。较高的测量数将以相对较低的成本完成，高技能 研究人员。 5。与动物模型核心（核心B）的密切相互作用将确保正确收集和存储 标本并增加可重复性。