Bioinflammation Core

生物炎症核心

• 批准号: 10094228
• 负责人: Jennifer C Sullivan
• 金额: $ 29.19万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10094228
• 关键词: Address; Animal Model; Applications Grants; Biological Assay; Blinded; Blood specimen; Cells; Chronic Kidney Failure; Clinical; Collection; Development; Ensure; Experimental Animal Model; Goals; HMGB1 Protein; Heart failure; Hypertension; IL17 gene; IL6 gene; Immune; Individual; Innate Immune Response; Interleukin-10; Investigation; Maintenance; Measurement; Measures; Methods; Mitochondrial DNA; Molecular; Myocardial Infarction; Pathogenesis; Pattern; Pattern recognition receptor; Peripheral arterial disease; Porcine Reproductive and Respiratory Syndrome; Preparation; Program Research Project Grants; Quality Control; Reagent; Records; Recovery; Reproducibility; Research Personnel; Risk; Role; Running; Sampling; Services; Specimen; Stroke; Structure; TLR4 gene; Tissue Sample; central database; cost; cytokine; experimental analysis; immune activation; novel; programs; success; synergism

BIOINFLAMMATION CORE SUMMARY CC, ERGUL The projects in this Program Project Grant application focus on two key damage associated molecular patterns (DAMPs), high mobility group box 1 (HMGB1) and mitochondrial (mt) DNA, activating the innate immune response via their respective pattern recognition receptors (PPRs) TLR4 and TLR9 resulting in stimulation of a number of cytokines in experimental animal models of hypertension. The Bioinflammation Core (Core C) is structured to serve all three projects with centralized measurements of DAMPs, PRRs, immune cells and downstream cytokines to facilitate the success of the individual projects and achieve the overall goals of this Program Project. The secondary goal is to provide necessary services to enhance the synergy among projects. Core C will be directed by Dr. Ergul and supported by Dr. Babak Baban. Both investigators have a long- standing record of analyzing experimental and clinical specimens with a highly qualified staff. A centralized Core is essential to the success of each project and the Program Project as a whole and offers the following benefits: 1. The coordination of sample preparation within the Bioinflammation Core C will benefit all Projects as Core C will optimize sample recovery and increase efficiency and rigor by allowing analysis of more samples in one run while providing blinded analyses of the samples. 2. Common approach (in many cases identical methods and reagents) to measure the same markers across the projects will provide synergy, quality control and consistency among the projects. 3. The measurement of primary and secondary DAMPs/PRRS in all Projects will cross cultivate the Projects and allow the development of new venues of investigation that otherwise would not have been possible with regular R01 applications. 4. Higher numbers of measurements will be completed at a relatively lower cost with highly skilled research personnel. 5. A close interaction with the Animal Models Core (Core B) will ensure proper collection and storage of specimens and increase reproducibility.

生物炎症核心总结CC，Ergul 本计划项目资助申请的项目集中在两个关键的损伤相关分子模式上 (DAMPS)、高迁移率族蛋白1(HMGB1)和线粒体(MT)DNA，激活天然免疫 通过其各自的模式识别受体(PPR)TLR4和TLR9进行反应，从而刺激 实验性高血压动物模型中细胞因子的数量。生物炎症核心(核心C)是 旨在为所有三个项目提供服务，集中测量DAMP、PRR、免疫细胞和 下游细胞因子促进个别项目的成功，并实现这一总体目标 计划项目。次要目标是提供必要的服务，以加强项目之间的协同作用。 核心C将由Ergul博士执导，Babak Baban博士提供支持。两位调查人员都有一个很长的- 拥有高素质员工分析实验和临床标本的长期记录。一个集中化的 核心对于每个项目和整个计划项目的成功至关重要，并提供以下内容 优势： 1.在生物炎症核心C内协调样品制备将使所有项目受益，因为 核心C将优化样品回收，并通过允许分析更多 一次采样，同时对样品进行盲法分析。 2.测量相同标记物的通用方法(在许多情况下是相同的方法和试剂) 所有项目将提供协同效应、质量控制和项目之间的一致性。 3.所有项目的一次和二次阻尼率/PRRS的测量将交叉培养 项目，并允许开发新的调查场所，否则就不会 可用于常规的R01应用程序。 4.将以相对较低的成本和高技能完成更多的测量 研究人员。 5.与动物模型核心(核心B)密切互动将确保适当收集和储存 并提高了重现性。