Live Imaging and Functional Evaluation Core

实时成像和功能评估核心

• 批准号: 10239006
• 负责人: Steven Nusinowitz
• 金额: $ 13.84万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10239006
• 关键词: Aging; Algorithmic Software; Angiography; Animal Model; Animals; Anterior; Applications Grants; Behavior; Behavioral; Behavioral Paradigm; Biological Assay; Biological Markers; Complement; Consultations; Contrast Sensitivity; Core Grant; Custom; Data; Data Analyses; Detection; Development; Disease; Electrophysiology (science); Electroretinography; Emerging Technologies; Engineering; Equipment; Evaluation; Eye; Fostering; Funding; Future; Goals; Gold; Grant; Growth; Hospitals; Image; Individual; Institutes; Intervention; Joints; Laboratories; Laboratory Research; Life; Light; Measurement; Methodology; Methods; Modality; Modification; Monitor; Motion; Mouse Strains; Mus; Mutant Strains Mice; Optical Coherence Tomography; Optics; Pathogenicity; Pattern; Pharmacology; Phenotype; Postdoctoral Fellow; Process; Psychophysics; Reflex action; Research; Research Design; Research Personnel; Research Project Grants; Resolution; Resources; Retina; Retinal Degeneration; Rodent; Services; Specialist; Structure; Swimming; System; Technology; Testing; The Sun; Time; TimeLine; Training; Translational Research; Update; Vertebrate Photoreceptors; Viral Vector; Vision; Vision research; Visual; Visual Acuity; Visual Pathways; Work; adaptive optics; angiogenesis; base; behavior test; cost; disease natural history; experience; experimental study; feasibility research; fluorescence imaging; fundus imaging; imaging capabilities; in vivo; in vivo imaging; instrumentation; interest; mouse model; new technology; non-invasive imaging; novel; programs; recruit; research study; response; retinal imaging; skills; small molecule; stem cells; vision science; visual performance

PROJECT SUMMARY – LIFE CORE The SEI Vision Research Group has a longstanding track record of using mouse models to study ocular processes and disorders. The in vivo characterization of these mouse models is carried out using the testing modalities provided by the LIFE Core. This resource has been available since 1999 as part of the Nusinowitz Laboratory and, since 2009, has been a component of the SEI Core Grant. With the growth of projects using a wide variety of mouse strains and mutants, and the expansion of translational research projects to modify or rescue ocular disorders, the LIFE component technologies are crucial for monitoring a broad range of experiments in living animals. The LIFE Core component will continue to offer a broad range of in vivo functional and structural testing customized to the needs of the investigator's research interests. The LIFE Core will be used to define the structural and functional phenotypes in acquired or newly generated mouse mutants to confirm whether they display the expected phenotypes, visual behavior and/or electrophysiological responses. In addition, investigators will continue to use the LIFE Core to document the natural history of disease in mouse models before they are used for interventional experiments, including those that are viral vector, stem cell, or pharmacologically based. The LIFE directors are committed to the provision of state-of-the-art non-invasive imaging and functional analysis of mouse models of ocular disease. Equipment and software algorithms are upgraded on a regular basis. We recently updated our Bioptigen sd-OCT to provide higher resolution retinal images and have added new optical bores to image the anterior segment in rodents. The latter is a new capability and highlights our interest in broadening the relevance of this resource to other investigators at SEI. In addition, we acquired a new Celeris Electrodiagnostics System for functional evaluation of the retina and visual pathway that will replace an aging custom-made system. Over the next study period we will expand the imaging capabilities by adding a Heidelberg HRA-II for wide-field imaging of fluorescent biomarkers and autofluorescence, and we will continue to work with our colleagues at Doheny Eye Institute, our affiliated eye hospital, to develop OCT-A for the mouse. Lastly, we will be adding behavioral testing of visual acuity and contrast sensitivity with the optokinetic reflex, and developing new behavioral paradigms to study rod and cone function in retinal degeneration.

项目概要-生命核心 SEI视觉研究小组在使用小鼠模型方面有着长期的记录 研究眼部过程和疾病。这些小鼠模型的体内表征是 使用LIFE Core提供的测试模式进行。该资源已 自1999年以来，作为Nusinowitz实验室的一部分，自2009年以来， 核心补助金的组成部分。随着项目的增长使用鼠标种类繁多 菌株和突变体，以及扩大转化研究项目以修饰或拯救 眼科疾病，LIFE组件技术是至关重要的监测范围广泛的 活体动物实验 LIFE核心组件将继续提供广泛的体内功能和 根据研究者的研究兴趣定制的结构测试。生命 核心将被用来定义结构和功能表型，在获得或新的 产生小鼠突变体，以确认它们是否显示预期的表型，视觉 行为和/或电生理反应。此外，调查人员将继续使用 LIFE Core用于在使用小鼠模型之前记录疾病的自然史 用于干预性实验，包括病毒载体、干细胞或 基于。 LIFE董事致力于提供最先进的非侵入性 眼部疾病小鼠模型的成像和功能分析。设备和软件 算法定期升级。我们最近更新了Bioptigen sd-OCT， 提供更高分辨率的视网膜图像，并增加了新的光学孔，以成像前 啮齿动物中的片段。后者是一种新的能力，突出了我们对扩大 这一资源与SEI的其他研究人员的相关性。此外，我们还收购了一家新的 用于视网膜和视觉通路功能评估的电诊断系统， 替换一个老化的定制系统在下一个研究阶段，我们将扩大成像 通过增加海德堡HRA-II荧光生物标记物的宽视野成像功能， 我们将继续与Doheny眼科研究所的同事合作， 附属眼科医院，开发OCT-A的小鼠。最后，我们将添加行为 用视动反射测试视力和对比敏感度，并开发新的 行为范例来研究视网膜变性中的视杆细胞和视锥细胞功能。