Live Imaging and Functional Evaluation (LIFE) Core

项目摘要 SEI视觉研究小组在使用鼠标模型方面有着长期的记录 用于研究眼睛的突起和紊乱。这些小鼠在体内的特性 模型在很大程度上是使用LIFE提供的测试模式进行的 核心组件。在一开始，LIFE核心组件受到了大量的 SEI教职员工的热情。随着使用各种各样的老鼠品系的项目的增长 和突变体以及翻译研究项目的扩展，在这些项目中，干预措施需要修改 或抢救眼疾正在调查中(见下文调查人员声明)， 组件技术对于监控广泛的动物实验至关重要， 从外视网膜的研究到对视觉信号传递途径的剖析 大脑皮层。作为其生产力的基准，LIFE核心组件直接为 在上一次赠款期间，在参考期刊上发表了29篇论文，还有许多正在进行的研究。 LIFE核心组件将继续提供广泛的功能和 结构测试，可以根据调查者的具体研究需要进行定制 兴趣、焦点和假设。与过去的授权期一样，LIFE核心将用于 确定获得性或新产生的小鼠突变体的结构和功能表型 或确认它们是否显示预期的表型、视觉行为和/或 电生理反应。调查人员将继续使用结构和功能 LIFE CORE技术在小鼠模型中记录疾病的自然历史 眼科疾病在用于干预实验之前，包括这些干预措施 以病毒载体、干细胞或药理为基础。 LIFE核心董事致力于提供最先进的活体成像 以及眼部疾病的动物模型和治疗的功能分析。为了实现这个目标， 定期升级设备，并创建自定义显示和协议 需要针对受疾病和/或干预影响的视觉通路中的特定位置。 在接下来的研究期间，我们将通过以下方式扩展生命核心的功能成像能力 包括几个新的和新的行为分析，已被引入SEI博士。 SamPath是我们基础科学部门的新成员，也是这个部门的联席主任 组件。这些扩展的行为分析的增加将为 LIFE核心的表型能力。

Project Summary The SEI Vision Research Group has a longstanding track record of using mouse models for the study of ocular processes and disorders. The in vivo characterization of these mouse models has, to a large extent, been carried out using the testing modalities provided by the LIFE Core Component. At its inception, the LIFE Core component was received with a great deal of enthusiasm by the SEI faculty. With the growth of projects using a wide variety of mouse strains and mutants and the expansion of translational research projects where interventions to modify or rescue ocular disorders is being investigated (see investigators statements below), the LIFE component technologies have been crucial for monitoring a broad range of animal experiments, from studies of the outer retina to the dissection of signal transmission pathways to the visual cortex. As a benchmark of its productivity, the LIFE Core component has contributed directly to 29 publications in referred journals over the last grant period, with numerous ongoing studies. The LIFE Core component will continue to offer a broad range of functional and structural testing that can be customized to the needs of the investigator's specific research interests, focus and hypotheses. As in the past grant period, the LIFE Core will be used to define the structural and functional phenotypes in acquired or newly generated mouse mutants or to confirm whether they display the expected phenotypes, visual behavior and/or electrophysiological responses. Investigators will continue to use the structural and functional technologies of the LIFE Core to document the natural history of disease in mouse models of ocular disease before they are used for intervention experiments, including those interventions that are viral vector, stem cell, or pharmacologically based. The LIFE core directors are committed to the provision of state-of-the-art in vivo imaging and functional analysis of animal models of ocular disease and treatments. Toward this goal, equipment is upgraded on a regular basis and custom displays and protocols are created as needed to target the specific site in the visual pathway affected by disease and/or intervention. Over the next study period we will expand the functional imaging capabilities of the LIFE core by including several new and novel behavioral assays that have been brought to SEI by Dr. Sampath, who is a new member of our Basic Science Division and a Co-Director of this component. The addition of these expanded behavioral assays will add a new dimension to the phenotyping capabilities of the LIFE Core.