Live Imaging and Functional Evaluation (LIFE) Core

项目摘要 SEI视觉研究小组在使用小鼠模型方面有着长期的记录 用于研究眼部过程和疾病。这些小鼠的体内表征 模型在很大程度上是使用LIFE提供的测试模式进行的 核心组件。在其成立之初，LIFE核心组件收到了大量的 热情的老师。随着使用各种小鼠品系的项目的增长 和突变体和扩大转化研究项目，其中干预，以修改 正在研究或抢救眼部疾病（见下文研究者声明），LIFE 组件技术对于监测广泛的动物实验至关重要， 从外层视网膜的研究到信号传输通路的解剖， 皮层作为其生产力的基准，LIFE核心组件直接有助于 在上一个资助期内，在引用的期刊上发表了29篇文章，其中有许多正在进行的研究。 LIFE核心组件将继续提供广泛的功能和 可以根据研究者的具体研究需要定制的结构测试 兴趣、焦点和假设。与过去的赠款期一样，LIFE核心将用于 定义获得的或新产生的小鼠突变体的结构和功能表型 或确认它们是否显示预期的表型、视觉行为和/或 电生理反应调查人员将继续使用结构和功能 LIFE核心的技术，以记录小鼠模型中疾病的自然史， 在用于干预实验之前， 它们是病毒载体、干细胞或基于DNA的。 LIFE核心董事致力于提供最先进的体内成像 以及眼部疾病和治疗的动物模型的功能分析。为了实现这一目标， 定期升级设备，并创建自定义显示和协议， 需要靶向受疾病和/或干预影响的视觉通路中的特定部位。 在下一个研究期间，我们将通过以下方式扩展LIFE核心的功能成像能力： 包括一些新的和新颖的行为分析，已带来了SEI博士。 Sampath是我们基础科学部的新成员，也是该项目的联合主任。 成分这些扩展的行为测定的增加将为本领域的研究增加新的维度。 LIFE Core的表型分析能力。

Project Summary The SEI Vision Research Group has a longstanding track record of using mouse models for the study of ocular processes and disorders. The in vivo characterization of these mouse models has, to a large extent, been carried out using the testing modalities provided by the LIFE Core Component. At its inception, the LIFE Core component was received with a great deal of enthusiasm by the SEI faculty. With the growth of projects using a wide variety of mouse strains and mutants and the expansion of translational research projects where interventions to modify or rescue ocular disorders is being investigated (see investigators statements below), the LIFE component technologies have been crucial for monitoring a broad range of animal experiments, from studies of the outer retina to the dissection of signal transmission pathways to the visual cortex. As a benchmark of its productivity, the LIFE Core component has contributed directly to 29 publications in referred journals over the last grant period, with numerous ongoing studies. The LIFE Core component will continue to offer a broad range of functional and structural testing that can be customized to the needs of the investigator's specific research interests, focus and hypotheses. As in the past grant period, the LIFE Core will be used to define the structural and functional phenotypes in acquired or newly generated mouse mutants or to confirm whether they display the expected phenotypes, visual behavior and/or electrophysiological responses. Investigators will continue to use the structural and functional technologies of the LIFE Core to document the natural history of disease in mouse models of ocular disease before they are used for intervention experiments, including those interventions that are viral vector, stem cell, or pharmacologically based. The LIFE core directors are committed to the provision of state-of-the-art in vivo imaging and functional analysis of animal models of ocular disease and treatments. Toward this goal, equipment is upgraded on a regular basis and custom displays and protocols are created as needed to target the specific site in the visual pathway affected by disease and/or intervention. Over the next study period we will expand the functional imaging capabilities of the LIFE core by including several new and novel behavioral assays that have been brought to SEI by Dr. Sampath, who is a new member of our Basic Science Division and a Co-Director of this component. The addition of these expanded behavioral assays will add a new dimension to the phenotyping capabilities of the LIFE Core.