Signal Integration During Eye Formation
眼睛形成过程中的信号整合
基本信息
- 批准号:10245151
- 负责人:
- 金额:$ 34.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAllelesAnophthalmosApoptosisBindingBioinformaticsBiologyBrainCancer BiologyCell MaintenanceCell PolarityCell ProliferationCell membraneCell surfaceCellsCiliaColobomaComplexConfocal MicroscopyCre driverDataDevelopmentDiffuseDiseaseEmbryoEmbryologyEnhancersEtiologyEyeEye AbnormalitiesEye DevelopmentGenerationsGenesGeneticGoalsGrowthHistologyHoloprosencephalyHourHumanHypothalamic structureImmunohistochemistryImpairmentIn Situ HybridizationIn VitroLigandsMicrophthalmosMolecularMorphogenesisNational Eye InstituteNeural RetinaNeuronsOptic DiskOptic NerveOptic vesicleOrganogenesisOrganoidsOuter Limiting MembranePathogenesisPathway interactionsPatternPituitary GlandProcessProductionRegulationReportingResearchRetinaRetinal ConeRetinal Ganglion CellsRoleSMO geneSepto-Optic DysplasiaSignal PathwaySignal TransductionSonic Hedgehog PathwaySpinal CordSubthalamic structureSystemTechniquesTechnologyTestingTimeTissuesTransgenic MiceTransgenic OrganismsUntranslated RNAVisualbasediencephalonexperimental studyeye formationgastrulationhistogenesisin vivoinduced pluripotent stem cellinsightlensmalformationmorphogensmouse geneticsmutantmutant mouse modelneurogenesisnotch proteinoptic cupoptic stalkreceptorreceptor expressionresponseretinal progenitor cellskin organogenesissmoothened signaling pathwayspatiotemporalstem cell biologystem cell growthstem cells
项目摘要
Project Summary:
A long-standing question in biology is how cells respond to multiple signaling inputs with a specific response.
This proposal investigates the intersections of Notch and Hh signaling pathways, which are widely required
during development, and basic mechanisms congenital eye disease. Both signaling systems regulate cell
proliferation, morphogenesis, cell polarity, differentiation, apoptosis and stem cell maintenance. However, the
spatiotemporal contexts for each differs at the cellular and tissue levels during optic vesicle morphogenesis
and growth. This proposal will use in vivo complex conditional (cre-lox) mouse genetics, mouse transgenics,
embryology, iPSC-derived retinal organoids, histology, immunohistochemistry, confocal microscopy, in situ
hybridization, qPCR and bioinformatics technologies to investigate basic, mechanistic questions about the
initiation and progression of eye formation from the embryonic brain. We will address several important
questions: 1) What are the spatial and temporal constraints for Notch versus Hh in controlling growth,
morphogenesis, patterning, tissue polarity and the timing of differentiation in the optic vesicle, optic cup, RPE
and optic stalk? 3) What are the optimal conditions by which Notch and Shh pathways contribute to the
generation of retinal ganglion cell and cone photoreceptor neurons in retinal organoid culture? 1) How do the
Notch and Hh pathways regulate the eye expression domains of a common target gene, Hes1?
项目总结:
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Nadean L Brown其他文献
Nadean L Brown的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Nadean L Brown', 18)}}的其他基金
2016 and 2018 Visual Systems Development Gordon Research Conference & Gordon Research Seminar
2016年和2018年视觉系统开发戈登研究会议
- 批准号:
9181439 - 财政年份:2015
- 资助金额:
$ 34.27万 - 项目类别:
2016 and 2018 Visual Systems Development Gordon Research Conference & Gordon Research Seminar
2016年和2018年视觉系统开发戈登研究会议
- 批准号:
9045122 - 财政年份:2015
- 资助金额:
$ 34.27万 - 项目类别:
Cell-Cell Signaling During Mammalian Early Eye Formation
哺乳动物早期眼睛形成过程中的细胞间信号传导
- 批准号:
7579777 - 财政年份:2008
- 资助金额:
$ 34.27万 - 项目类别:
Cell-Cell Signaling During Mammalian Early Eye Formation
哺乳动物早期眼睛形成过程中的细胞间信号传导
- 批准号:
8427501 - 财政年份:2008
- 资助金额:
$ 34.27万 - 项目类别:
Cell-Cell Signaling During Mammalian Early Eye Formation
哺乳动物早期眼睛形成过程中的细胞间信号传导
- 批准号:
7367571 - 财政年份:2008
- 资助金额:
$ 34.27万 - 项目类别:
相似海外基金
Linkage of HIV amino acid variants to protective host alleles at CHD1L and HLA class I loci in an African population
非洲人群中 HIV 氨基酸变异与 CHD1L 和 HLA I 类基因座的保护性宿主等位基因的关联
- 批准号:
502556 - 财政年份:2024
- 资助金额:
$ 34.27万 - 项目类别:
Olfactory Epithelium Responses to Human APOE Alleles
嗅觉上皮对人类 APOE 等位基因的反应
- 批准号:
10659303 - 财政年份:2023
- 资助金额:
$ 34.27万 - 项目类别:
Deeply analyzing MHC class I-restricted peptide presentation mechanistics across alleles, pathways, and disease coupled with TCR discovery/characterization
深入分析跨等位基因、通路和疾病的 MHC I 类限制性肽呈递机制以及 TCR 发现/表征
- 批准号:
10674405 - 财政年份:2023
- 资助金额:
$ 34.27万 - 项目类别:
An off-the-shelf tumor cell vaccine with HLA-matching alleles for the personalized treatment of advanced solid tumors
具有 HLA 匹配等位基因的现成肿瘤细胞疫苗,用于晚期实体瘤的个性化治疗
- 批准号:
10758772 - 财政年份:2023
- 资助金额:
$ 34.27万 - 项目类别:
Identifying genetic variants that modify the effect size of ApoE alleles on late-onset Alzheimer's disease risk
识别改变 ApoE 等位基因对迟发性阿尔茨海默病风险影响大小的遗传变异
- 批准号:
10676499 - 财政年份:2023
- 资助金额:
$ 34.27万 - 项目类别:
New statistical approaches to mapping the functional impact of HLA alleles in multimodal complex disease datasets
绘制多模式复杂疾病数据集中 HLA 等位基因功能影响的新统计方法
- 批准号:
2748611 - 财政年份:2022
- 资助金额:
$ 34.27万 - 项目类别:
Studentship
Recessive lethal alleles linked to seed abortion and their effect on fruit development in blueberries
与种子败育相关的隐性致死等位基因及其对蓝莓果实发育的影响
- 批准号:
22K05630 - 财政年份:2022
- 资助金额:
$ 34.27万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Genome and epigenome editing of induced pluripotent stem cells for investigating osteoarthritis risk alleles
诱导多能干细胞的基因组和表观基因组编辑用于研究骨关节炎风险等位基因
- 批准号:
10532032 - 财政年份:2022
- 资助金额:
$ 34.27万 - 项目类别:
Investigating the Effect of APOE Alleles on Neuro-Immunity of Human Brain Borders in Normal Aging and Alzheimer's Disease Using Single-Cell Multi-Omics and In Vitro Organoids
使用单细胞多组学和体外类器官研究 APOE 等位基因对正常衰老和阿尔茨海默病中人脑边界神经免疫的影响
- 批准号:
10525070 - 财政年份:2022
- 资助金额:
$ 34.27万 - 项目类别:
Leveraging the Evolutionary History to Improve Identification of Trait-Associated Alleles and Risk Stratification Models in Native Hawaiians
利用进化历史来改进夏威夷原住民性状相关等位基因的识别和风险分层模型
- 批准号:
10689017 - 财政年份:2022
- 资助金额:
$ 34.27万 - 项目类别: