Core 2: Pathology and Tissue Core

芯2 具体目标 M.D.的主要目标安德森癌症中心（MDACC）的白血病孢子是改善 治疗白血病患者。实现这一目标的一个基本组成部分是开展有重点的 涉及人体组织和血液标本的转化研究，允许对靶生物学进行研究 和正常组织，评估对靶组织和正常组织的治疗效果，以及调节相关的 生物标志物。为此，白血病样本分布、库和阵列分析核心（以下简称核心） 2）将收集、处理和保存来自患者的人体组织标本，并将这些组织分散到 白血病孢子研究者。该核心还将提供RNA和蛋白质的全基因组分子图谱 表达，这提供了对细胞生理学的见解，如信号传导途径的活动。 AML组织样本、分发、库存和阵列分析核心具有以下目标： 1.开发和维护血液和骨骼中完整细胞、血清、DNA、RNA和蛋白质的储存库 从MDACC的AML患者中获得的骨髓标本。 2.维护一个全面的、前瞻性的、交互式的在线数据库， 核心收到的样本数据。 3.提供AML患者样本的反相蛋白质阵列（RPPA），用于蛋白质筛查 在大量白血病细胞、白血病干细胞和间充质基质细胞（MSC）中表达。 4.为基因表达谱（GEP）提供RNA-Seq数据解释。 5.通过共享血液和骨髓资源促进白血病孢子间和孢子外的合作。

Core 2 Specific Aims The primary objective of the M.D. Anderson Cancer Center (MDACC) Leukemia Spore is to improve the treatment of patients with leukemia. A fundamental component to meeting this objective is the conduct of focused translational research involving human tissue and blood specimens, allowing investigation of the biology of target and normal tissues, evaluation of treatment effects on both target and normal tissues, and modulation of relevant biomarkers. To these ends, the Leukemia Sample Distribution, Banking and Array Profiling Core (hereafter Core 2), will collect, process and maintain human tissue specimens from patients and will disperse these tissues to Leukemia Spore investigators. The Core will also provide genome-wide molecular profiling of RNA and protein expression, which provide insights into cellular physiology such as signaling pathway activity. The AML Tissue Sample, Distribution, Banking and Array Profiling Core has the following objectives: 1. Develop and maintain a repository of intact cells, serum, DNA, RNA and protein from blood and bone marrow specimens obtained from AML patients at MDACC. 2. Maintain a comprehensive, prospective, interactive online database with detailed clinical and pathologic data for the samples received by the Core. 3. Provide Reverse Phase Protein Arrays (RPPA) from AML patient samples for screening of protein expression in bulk leukemia cells, leukemia stem cells, and mesenchymal stromal cells (MSC). 4. Provide RNA-Seq data interpretation for gene expression profiling (GEP). 5. Facilitate inter- and extra- Leukemia Spore collaborations through sharing of blood and marrow resources.