Pathogenesis of Helicobacter pylori infection
幽门螺杆菌感染的发病机制
基本信息
- 批准号:10250299
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:AcidsAdherenceBacteriaBacterial AdhesinsBacterial ProteinsBar CodesCancer EtiologyCarcinogensCaringCellsCessation of lifeClinicalDevelopmentDiseaseDistalDuodenal UlcerEpithelial CellsFamilyGastric AdenocarcinomaGastric lymphomaGastric mucosaGastric ulcerGenesGenomeGoalsGram-Negative BacteriaHelicobacter InfectionsHelicobacter pyloriHigh-Throughput Nucleotide SequencingHumanIndividualInfectionInflammatory ResponseInvestigationLabelLeadLibrariesMediatingMembrane ProteinsMethodsMolecularMusMutagenesisMutationNucleotidesPathogenesisPeptic UlcerPersonsPharmaceutical PreparationsPopulationPrevention approachPrevention strategyProcessProteinsRegimenReportingReverse Transcriptase Polymerase Chain ReactionRiskRoleSignal TransductionStomachStomach DiseasesSurfaceSystemTravelUnited StatesUnited States Department of Veterans AffairsVeteransWorkWorld Health Organizationcancer riskdisorder preventionexperimental studyhigh riskin vivoinsightmalignant stomach neoplasmmembermutantnovel strategiespreventpromotertranscriptometranscriptome sequencing
项目摘要
Helicobacter pylori is a Gram-negative bacterium that colonizes the human stomach. H. pylori infection is
associated with an increased risk of cancer of the distal stomach, as well as peptic ulcer disease. The World
Health Organization has classified H. pylori as a type I carcinogen, and gastric cancer is the third leading
cause of cancer-related death worldwide. The long-term goals of this work are to understand the molecular
mechanisms that allow H. pylori to persistently colonize the human gastric mucosa, to understand the
molecular mechanisms by which H. pylori infection leads to the development of gastric cancer or peptic
ulceration, and to develop effective strategies for the prevention of these diseases. To achieve these long-term
goals, we seek to understand the actions of bacterial proteins that are localized on the surface of H. pylori. H.
pylori genomes contain more than 50 genes that are predicted to encode outer membrane proteins (OMPs).
Several OMPs have been reported to mediate H. pylori adherence to gastric epithelial cells, but the functions
of most H. pylori OMPs are not known. The overall hypothesis of this proposal is that H. pylori utilizes
specific OMPs at various stages of the infectious process to optimize initial colonization of the stomach and to
facilitate persistent colonization in the presence of a gastric mucosal inflammatory response, thereby
contributing to the development of gastric disease. The specific aims are (i) To define the role of two-
component signal transduction systems (TCSs) in regulating genes encoding OMPs, (ii) To define OMPs that
have a dominant role in promoting H. pylori colonization of the stomach, and (iii) To define temporal features of
processes by which specific OMPs promote H. pylori colonization of the stomach and modulate development
of gastric disease. To accomplish Aim 1, we will compare the transcriptomes of wild-type and mutant strains,
using RNA-seq and quantitative RT-PCR methods. To accomplish Aim 2, we will infect mice with a library of
strains containing mutations in OMP-encoding proteins, each labeled with a distinct nucleotide bar code, and
then will use high throughput sequencing to analyze the bacterial populations colonizing the stomach. To
accomplish Aim 3, we will regulate the expression of selected OMP-encoding genes in vivo through use of an
inducible promoter. Collectively, these experiments will provide important new insights into the roles of specific
OMPs in promoting initial colonization of the stomach, persistence and disease.
幽门螺杆菌是一种革兰氏阴性细菌,在人的胃中定植。幽门螺杆菌感染是
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TIMOTHY L COVER其他文献
TIMOTHY L COVER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TIMOTHY L COVER', 18)}}的其他基金
Helicobacter pylori cag Pathogenicity Island and Gastric Carcinogenesis
幽门螺杆菌致病岛与胃癌发生
- 批准号:
8413059 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Regulation of H. Pylori Virulance by Dietary Factors that Impact Gastric Cancer
影响胃癌的饮食因素对幽门螺杆菌毒力的调节
- 批准号:
9274163 - 财政年份:2009
- 资助金额:
-- - 项目类别:
相似海外基金
A development of implant surface modification preventing the adherence of peri-implantitis related bacteria
种植体表面改性的发展可防止种植体周围炎相关细菌的粘附
- 批准号:
19K19141 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Early-Career Scientists
Development of evaluation system for systemic infectious disease by oral bacteria based on the adherence to host tissues
基于宿主组织粘附性的口腔细菌全身感染性疾病评价系统的开发
- 批准号:
23593093 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Interactionbetween salivary proteins andoral bacteria: determinants for bacterial adherence and aggregation
唾液蛋白和口腔细菌之间的相互作用:细菌粘附和聚集的决定因素
- 批准号:
23659987 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
A study for the adherence of pathogens for the periodontal disease to the dental materials and for the inhibition effect of specific antibodies to the bacteria
牙周病病原体在牙科材料上的附着以及特异性抗体对该细菌的抑制效果的研究
- 批准号:
21592660 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional and structural identification of peptidoglycan part structures involved in thrombospondin-1 mediated adherence of Gram-positive bacteria to host cells
参与血小板反应蛋白-1介导的革兰氏阳性菌与宿主细胞粘附的肽聚糖部分结构的功能和结构鉴定
- 批准号:
64038601 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Research Grants
Deciphering the molecular mechanisms of Streptococcus pneumoniae adherence and bacteria-induced host cell signalling
破译肺炎链球菌粘附和细菌诱导的宿主细胞信号传导的分子机制
- 批准号:
83883037 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Research Grants
A study of mechanism of adherence to the oral cavity in periodontopathic bacteria by animal model.
牙周病细菌口腔黏附机制的动物模型研究。
- 批准号:
15592202 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on molecular basis of bacteria-host cell interaction in enteropathogenic Escherichia coli adherence.
致病性大肠杆菌粘附中细菌-宿主细胞相互作用的分子基础研究。
- 批准号:
14570236 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of inhibitory peptide in adherence of periodontopathic bacteria to dental plaqu
牙周病细菌粘附牙菌斑抑制肽的研制
- 批准号:
09557175 - 财政年份:1997
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (B)
Factors affecting adherence of bacteria in diarrhoeal d isease due to E.coli
影响大肠杆菌腹泻病细菌粘附的因素
- 批准号:
nhmrc : 920652 - 财政年份:1992
- 资助金额:
-- - 项目类别:
NHMRC Project Grants














{{item.name}}会员




