LARGE SCALE T CELL EPITOPE DISCOVERY - COVID Supplement

SARS-CoV-2表位巨池（SARS-CoV-2 MPs）用于研究SARS-CoV-2 CD4和CD8特异性T细胞反应。Megapool （MP）方法允许同时测试大量的表位。根据这种方法，大量不同的表位被溶解、池化和再冻干，以避免与高浓度DMSO相关的细胞毒性问题，通常在单个预溶解的表位池化时遇到。这些MPs已被用于CD4和CD8 T细胞表位鉴定。

The SARS-CoV-2 epitope Megapools (SARS-CoV-2 MPs) is used to study SARS-CoV-2 CD4 and CD8 specific T cell responses. The Megapool (MP) approach allows simultaneous testing of large number of epitopes. According to this approach large numbers of different epitopes are solubilized, pooled and re-lyophilized to avoid cell toxicity problems associated with high concentrations of DMSO typically encountered when single pre-solubilized epitopes are pooled. These MPs have been used for both CD4 and CD8 T cell epitope identification.