Development of an integrated smartphone/resistive nanosensor for onsite biomonitoring of exposure to pesticides

开发集成智能手机/电阻纳米传感器，用于农药暴露现场生物监测

基本信息

批准号：
10255622
负责人：
Jun Wang
金额：
$ 25.59万
依托单位：
NANODIAGNOSTIC TECHNOLOGY, LLC
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-01 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10255622
关键词：
Accident and Emergency department Acute Address Agriculture Area Biological Assay Biological Markers Biological Monitoring Blood Blood specimen Carbon Nanotubes Cellular Phone Cessation of life Chlorpyrifos Cholinesterases Chronic Data Data Analyses Data Reporting Detection Development Devices Diazinon Disasters Dose Drops Environmental Health Environmental and Occupational Exposure Enzyme Inhibition Enzymes Epidemiologist Exposure to Farming environment Fingers Foundations Goals Health Home Human Human Resources In Vitro Industry Insecticides Intervention Laboratories Letters Measurement Measures Methods Monitor Nanotechnology National Institute of Environmental Health Sciences Occupational Occupational Health Organophosphates Performance Pesticides Phase Physicians&apos Offices Poisoning Population Property Reproducibility Research Research Project Grants Risk Sampling Schools Site Small Business Technology Transfer Research Students Surface Technology Testing Time Toxic effect Toxicology Translating Treatment Efficacy United States Wireless Technology Work acute symptom clinical care clinically relevant cloud platform commercialization cost cost effective data management data sharing data sharing networks detection limit farm worker global health glucose monitor innovation interest mHealth meter minimally invasive mobile application nanosensors nanowire novel novel diagnostics organophosphate poisoning pesticide exposure pesticide poisoning point of care point-of-care diagnostics polyaniline portability programs prototype rapid detection response self testing software development statistics tool toxic organophosphate insecticide exposure

项目摘要

Project Summary Organophosphate (OP) pesticides poisoning is a major environmental and occupational health problem worldwide due to its acute or chronic toxicity. Rapid and accurate detection of personal exposure to OP pesticides is critical to both research and clinical care for victims. However, this detection remains highly variable across commercially available assays, e.g., the Ellman method, and such assays may also not adequately detect low but clinically relevant levels of exposure biomarkers. Moreover, these assays are generally performed in the centralized laboratories, and is slow and expensive. These deficits translate into missed opportunities for the timely initiation of the most appropriate treatment and interventions for acutely or chronically poisoned victims. A simple, rapid, non- or minimally invasive biomonitoring tool for onsite sensitive and accurate detection of personal exposure to OP pesticides is highly desirable and fits the interest of NIEHS exposure program. This STTR phase I project is to develop an innovative integrated smartphone/nanosensor platform for onsite rapid and sensitive detection of OP exposure using a tiny drop of finger-stick blood. This nanosensor will take advantage of nanotechnology and wireless technology and utilize an important biomarker, blood cholinesterase, for the detection of personal OP exposure and the resulted tool has the potential to serve as mHealth for environmental and occupational workers. The research will determine the detection limit, sensitivity, dynamic range, and other key performance metrics of the sensing platform using blood samples in vitro dosed with typical pesticides and validate the platform in field using finger-stick blood samples from farmworkers, and demonstrate the feasibility of the platform for sensitive and accurate detection of exposure to OP pesticides. The resulted nanosensor would be a simple, rapid, cost-effective, and minimally-invasive, point-of-care biomonitoring tool for accurate personal exposure assessment and for on-site rapid detection of exposure to OP pesticides. Thus, this novel sensing platform could serve as a personal meter for self-test just like a glucose meter and could also be used in field, physician's office, bedside, and emergency room.

项目摘要有机磷酸盐（OP）农药中毒是全球主要的环境和职业健康问题由于其急性或慢性毒性。快速准确地发现个人接触OP农药的暴露是对受害者的研究和临床护理至关重要。但是，该检测在整个环境中仍然很大变化市售测定法，例如Ellman方法和此类测定法可能也无法充分检测到低但是临床相关的暴露生物标志物水平。而且，这些测定通常在集中实验室，缓慢而昂贵。这些赤字转化为错过的机会及时开始对急性或长期中毒的受害者的最合适的治疗和干预措施。一种简单，快速，非或微创生物监测工具，用于现场敏感，准确检测个人接触OP农药是非常可取的，并且适合NIEHS暴露计划的兴趣。这 STTR I期项目将开发一个创新的集成智能手机/纳米传感器平台，用于现场快速和使用一小滴手指粘的血液对OP暴露的敏感检测。该纳米传感器将利用纳米技术和无线技术，并利用重要的生物标志物血液胆碱酯酶进行检测个人OP暴露和由此产生的工具有可能充当环境的MHealth和职业工人。该研究将确定检测极限，灵敏度，动态范围和其他密钥感应平台的性能指标，使用典型农药和使用农场工人的手指刺血样品在现场验证平台，并证明可行性敏感，准确检测暴露于OP农药的平台。由此产生的纳米传感器将是一种简单，快速，成本效益和最小侵入性生物监测工具，可准确个人暴露评估和现场快速检测对OP农药的暴露。因此，这本小说传感平台可以像葡萄糖表一样用作自我测试的个人电表，也可以用于现场，医师办公室，床边和急诊室。