Multistage LC-MSn for automated glycan isomer assignment of glycopeptides

用于糖肽自动聚糖异构体分配的多级 LC-MSn

• 批准号: 10255170
• 负责人: Miklos Guttman
• 金额: $ 25.66万
• 依托单位: PROTEIN METRICS, LLC
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-16 至 2022-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10255170
• 关键词: Address; Adopted; Biological; Biological Response Modifier Therapy; Biology; Catalogs; Cells; Characteristics; Communities; Complex; Computer software; Data; Databases; Detection; Development; Diagnostic; Discrimination; Disease; Dissociation; Future; Glycobiology; Glycopeptides; Glycoproteins; Immune response; Immune system; Institutes; Ions; Isomerism; Laboratories; Letters; Libraries; Link; Mass Fragmentography; Mass Spectrum Analysis; Methods; Milk; Monitor; Monosaccharides; Noise; Oligosaccharides; Onset of illness; Organism; Outcome; Output; Pattern; Peptides; Phase; Play; Polysaccharides; Post-Translational Protein Processing; Protein Glycosylation; Proteins; Proteomics; Protocols documentation; Regulation; Reproducibility; Research Personnel; Role; Scanning; Signal Transduction; Site; Small Business Technology Transfer Research; Software Tools; Structure; Testing; Therapeutic; Time; Work; base; cancer cell; carbohydrate structure; glycoproteomics; glycosylation; immunoregulation; implementation tool; insight; instrumentation; pathogen; prevent; protein aminoacid sequence; prototype; stereochemistry; sugar; tandem mass spectrometry; tool; tumor progression; user-friendly

N- and O-linked glycosylation are some of the most common, abundant, and biologically important posttranslational modifications of proteins, yet they are also some of the most difficult to study. The dominant analytical platform for the study of glycosylation is mass spectrometry, yet this platform on its own is incomplete, because it cannot elucidate glycan topology and linkage stereochemistry. This project will advance the use of mass spectrometry for glycan structure determination, especially for the case of O-linked glycans, by studying the ion propensities of various structural motifs in multi-stage ion trap mass spectrometry, and by building new software for structure inference from such mass spectra.

N-和O-连接的糖基化是一些最常见的，丰富的，生物学上的， 重要的蛋白质翻译后修饰，但它们也是一些最困难的 学习用于糖基化研究的主要分析平台是质谱法， 然而，这个平台本身是不完整的，因为它不能阐明聚糖拓扑结构， 键立体化学这个项目将促进质谱法在聚糖中的应用 结构测定，特别是对于O-连接聚糖的情况，通过研究离子 多阶段离子阱质谱中各种结构基序的倾向，以及 建立新的软件来从这样的质谱推断结构。