Multistage LC-MSn for automated glycan isomer assignment of glycopeptides

用于糖肽自动聚糖异构体分配的多级 LC-MSn

• 批准号: 10255170
• 负责人: Miklos Guttman
• 金额: $ 25.66万
• 依托单位: PROTEIN METRICS, LLC
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-16 至 2022-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10255170
• 关键词: Address; Adopted; Biological; Biological Response Modifier Therapy; Biology; Catalogs; Cells; Characteristics; Communities; Complex; Computer software; Data; Databases; Detection; Development; Diagnostic; Discrimination; Disease; Dissociation; Future; Glycobiology; Glycopeptides; Glycoproteins; Immune response; Immune system; Institutes; Ions; Isomerism; Laboratories; Letters; Libraries; Link; Mass Fragmentography; Mass Spectrum Analysis; Methods; Milk; Monitor; Monosaccharides; Noise; Oligosaccharides; Onset of illness; Organism; Outcome; Output; Pattern; Peptides; Phase; Play; Polysaccharides; Post-Translational Protein Processing; Protein Glycosylation; Proteins; Proteomics; Protocols documentation; Regulation; Reproducibility; Research Personnel; Role; Scanning; Signal Transduction; Site; Small Business Technology Transfer Research; Software Tools; Structure; Testing; Therapeutic; Time; Work; base; cancer cell; carbohydrate structure; glycoproteomics; glycosylation; immunoregulation; implementation tool; insight; instrumentation; pathogen; prevent; protein aminoacid sequence; prototype; stereochemistry; sugar; tandem mass spectrometry; tool; tumor progression; user-friendly

N- and O-linked glycosylation are some of the most common, abundant, and biologically important posttranslational modifications of proteins, yet they are also some of the most difficult to study. The dominant analytical platform for the study of glycosylation is mass spectrometry, yet this platform on its own is incomplete, because it cannot elucidate glycan topology and linkage stereochemistry. This project will advance the use of mass spectrometry for glycan structure determination, especially for the case of O-linked glycans, by studying the ion propensities of various structural motifs in multi-stage ion trap mass spectrometry, and by building new software for structure inference from such mass spectra.

N-和o -链糖基化是最常见、最丰富和最具生物学意义的