Elucidating sympathetic vasoconstrictor mechanisms of autonomic dysreflexia in persons with spinal cord injury

阐明脊髓损伤患者自主神经反射异常的交感血管收缩机制

• 批准号: 10257978
• 负责人: DEAN L KELLOGG
• 金额: --
• 依托单位: SOUTH TEXAS VETERANS HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10257978
• 关键词: Acute; Address; Adrenal Glands; Adrenergic alpha-Antagonists; Agonist; Antihypertensive Agents; Attenuated; Autonomic Dysreflexia; Baroreflex; Bladder; Blood Pressure; Blood Pressure Monitors; Blood Vessels; Blood flow; Cardiovascular Diseases; Cardiovascular system; Catecholamines; Cause of Death; Cervical; Chest; Clinical Trials; Clonidine; Cutaneous; Data; Development; Dose; Epinephrine; Forearm; Functional disorder; Future; Goals; Guide prevention; Hypersensitivity; Hypotension; Immune System Diseases; Immunity; Impairment; Incidence; Infection; Injury; Intervention; Intracranial Hemorrhages; Iontophoresis; Laser-Doppler Flowmetry; Lead; Lesion; Life; Measures; Methods; Modeling; Monitor; Morbidity - disease rate; Myocardial Infarction; Nerve; Neurons; Nifedipine; Nitroglycerin; Norepinephrine; Pathology; Percussion; Peripheral; Persons; Pharmacological Treatment; Pharmacology; Phentolamine; Phenylephrine; Prevention; Preventive treatment; Prophylactic treatment; Recurrence; Role; Seizures; Site; Skin; Spinal; Spinal cord injury; Stimulus; Structure; Synapses; Synaptic Receptors; Techniques; Testing; Therapeutic Intervention; Vascular resistance; Vasoconstrictor Agents; Vasomotor; alpha 2 agonist; alpha-adrenergic receptor; arteriole; blood pressure regulation; clinical care; comparative; design; fighting; immune function; mortality; mortality risk; neuropeptide Y; noradrenergic; novel; presynaptic; prevent; preventive intervention; receptor; relating to nervous system; response; reuptake; side effect; targeted treatment; theories; uptake; vasoconstriction

Autonomic dysreflexia (AD) is a potentially life-threatening consequence of cervical and high thoracic (above T6) spinal cord injury (SCI) characterized by abruptly high elevations in blood pressure in response to noxious stimuli below the injury level. 1,2 In addition to the potential morbidity caused by acute rise in blood pressure, recurrent AD over a longer period has been associated with changes in vascular structure that lead to CVD 3,4and impaired immune function, 5 which are both leading causes of mortality in persons with SCI. 6 While peripheral arteriolar vasoconstriction (VC) is documented to be pronounced during AD,7,8 The pathophysiological mechanisms of this VC are not well defined. A few proposed mechanisms include: 1) increase in neuronal release of norepinephrine (NE) and co-transmitters (CT); 2) post-junctional alpha-receptor hypersensitivity; 3) impaired reuptake of NE and/or 4) increases in circulating catecholamines (Epinephrine > NE) from adrenal stimulation.2,9 None of these mechanisms has been fully proven or is widely accepted. Furthermore, there are no known effective preventative or targeted treatment of the underlying pathophysiology of the AD, as the mechanisms are unknown. Current clinical care of AD with pharmacologic therapies is not without potential for significant side effects. Pharmacologic treatment includes short acting anti- hypertensives (e.g.; nitroglycerin and nifedipine), which can cause systemic hypotension. There is no known treatment targeting the underlying arteriolar vasoconstriction mechanisms of AD since they are unknown. Elucidating the underlying pathophysiology will facilitate the development of targeted treatment(s) to attenuate AD without significant adverse side effects. Our proposal aims to take the next step in elucidating the underlying pathophysiology of VC during AD. We aim to test one of the proposed mechanisms using a novel non-invasive technique never before used in studies testing VC pathology during AD. Specifically we will test following hypotheses: 1) Blockade of pre-synaptic neural release of NE and CT will abolish cutaneous VC during AD and 2) blockade of post synaptic alpha adrenergic receptors will decrease but not fully abolish VC during AD. Arterioles of the skin are easily accessible to test these hypotheses. We will use a novel and non- invasive technique of local iontophoretic delivery of pharmacologic agents combined with skin blood flow monitoring by laser Doppler flowmetry (LDF) and mean blood pressure (MAP) monitoring to define the underlying pathophysiology.10 Specific AIM 1 will compare the impact of a sympathetic neuronal blocking agent (bretylium = BT; blocks release of NE and co-transmitters from sympathetic noradrenergic nerves)) on cutaneous vascular conductance (CVC=LDF/MAP) during an AD episode at BT treated and control (CON) sites on a forearm and posterior calf. CVC will be compared at baseline = BL at normotension then every 20 seconds during AD stimulated by bladder percussion. If the increased arteriolar vasoconstriction is due to increased neuronal release of NE and CT then the sites treated with BT will demonstrate comparatively less vasoconstriction. Specific AIM 2 will evaluate the impact of non-selective alpha-adrenergic receptor blockade (phentolamine – PT) on CVC during AD. Before using PT during AD, the optimal PT dose to block alpha receptors will be determined by utilizing alpha 1 and 2 agonists with PT. After the PT dose required for total alpha –adrenergic blockade is determined, similar to AIM 1, CVC will be measured at PT treated and adjacent untreated CON sites. If the increased arteriolar vasoconstriction is due to NE release, then the CVC changes of PT and CON sites will differ. Ultimately, elucidating this information will 1) guide treatment, and potentially prophylaxis, to better target the underlying pathophysiology of VC during AD, with less systemic side effects and 2) help prevent the vascular adaptations with potential to contribute to CVD and impaired immunity associated with recurrent AD that both may increase mortality.

自主神经反射障碍（AD）是颈椎病和高胸椎病的潜在威胁