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Fully defined matrices for organoid culture

用于类器官培养的完全定义的基质

基本信息

批准号：
10257860
负责人：
Stefan Roberts
金额：
$ 29.08万
依托单位：
INSOMA BIO, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-21 至 2023-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10257860
关键词：
3-Dimensional Address Amino Acid Sequence Appearance Biocompatible Materials Biological Factors Biological Sciences Body Temperature Bone Marrow Brain Caliber Cell Communication Cell Culture Techniques Cell Death Cell Line Cell Survival Cells Cessation of life Collagen Cultured Cells Development Disease Doctor of Philosophy Engineered Gene Engineering Ensure Enteral Exhibits Extracellular Matrix Formulation Generations Growth In Vitro Industry Standard Injury Intellectual Property Interleukins Laboratories Lead Libraries Liquid substance Liver Mesenchymal Stem Cells Methods Modeling Normal tissue morphology North Carolina Organoids Pancreas Pharmacologic Substance Phase Phase Transition Phenotype Physiology Polymers Porosity Property Recombinant Proteins Recombinants Reporting Research Research Personnel Solid Stains Structure System Tertiary Protein Structure Testing Tissue Engineering Tissues Universities Variant base cold temperature commercialization crosslink design experimental study gastrointestinal in vivo in vivo Model matrigel preservation programs rapid growth relating to nervous system scaffold screening stem cells stemness success three dimensional cell culture tool transcriptomics two-dimensional

项目摘要

Project Summary Compared to standard two dimensional methods, three dimensional cell culture presents an opportunity to more reliably replicate and model in vivo physiology under controlled laboratory conditions. Organoid culture in particular has been shown to replicate in vivo tissue development, differentiation, and disease physiology in many cases. Recent reports however indicate that current cell culture substrates, including Matrigel, collagen and other recombinant extracellular matrix components, trigger undesirable phenotypes for modeling normal tissue function using cultured organoids. Here, we propose a collaborative Phase I program to expand the utility of inSoma’s proprietary Partially Ordered Polymers (POPs) in three-dimensional organoid culture with Scott Magness, PhD of the University of North Carolina at Chapel Hill, a leading expert in modeling gastrointestinal tissues using three dimensional culture systems. Our proof-of-concept program is intended to optimize POP formulations for three-dimensional culture, evaluate their support of organoid viability and proliferation, and employ transcriptomics to determine if aberrant phenotypes are reduced compared to other matrices, such as Matrigel. Once we establish lead formulations of the POP material for organoid culture, a Phase II program would evaluate organoids from other tissue types, including neural, pancreatic and liver tissues as well as potential testing of POPs as a support matrix for in vivo tissue engineering approaches. Each of these product classes has unique markets in both academic research, pharmaceutical screening and tissue engineering. inSoma has a robust intellectual property estate on POP biomaterials and given the rapid growth of three-dimensional culture for modeling in vivo physiology, we anticipate the product to address a significant market in the life science tools sector with specific advantages over market leaders such as Matrigel.

项目摘要与标准的二维方法相比，三维细胞培养提供了一个机会，在受控的实验室条件下更可靠地复制和模拟体内生理学。类器官培养特别是已经显示在体内复制组织发育、分化和疾病生理学，很多案例。然而，最近的报道表明，目前的细胞培养基质，包括基质胶、胶原蛋白，和其他重组细胞外基质成分，触发不期望的表型，用于模拟正常使用培养的类器官的组织功能。在这里，我们提出了一个合作的第一阶段计划，以扩大实用程序 inSoma的专利部分有序聚合物（POP）在三维类器官培养与斯科特位于查佩尔山的北卡罗来纳州大学的Magness博士说，组织使用三维培养系统。我们的概念验证计划旨在优化POP 用于三维培养的制剂，评估它们对类器官活力和增殖的支持，以及使用转录组学来确定与其他基质相比，异常表型是否减少，基质胶一旦我们建立了用于类器官培养的POP材料的主要配方，第二阶段计划将评估来自其他组织类型的类器官，包括神经，胰腺和肝脏组织以及潜在的测试持久性有机污染物作为体内组织工程方法的支持基质。这些产品类别中的每一个在学术研究、药物筛选和组织工程方面都有独特的市场。inSoma拥有在POP生物材料方面拥有强大的知识产权，并考虑到三维培养的快速发展，对于体内生理学建模，我们预计该产品将在生命科学工具领域占据重要市场与Matrigel等市场领导者相比具有特定优势。