Wirelessly controlled BSL3 vivarium system for automated microdosing in studies of infectious diseases

无线控制的 BSL3 饲养系统，用于传染病研究中的自动微剂量给药

• 批准号: 10259431
• 负责人: TUAN Q HOANG
• 金额: $ 127.31万
• 依托单位: FLUID SYNCHRONY, LLC
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10259431
• 关键词: 2019-nCoV; Academia; Acute; Address; Adrenal Cortex Hormones; Affect; Air; Animal Experimentation; Animal Model; Animals; Anti-Inflammatory Agents; Antibody Response; Automation; Bacteria; Biological Availability; Bluetooth; Breeding; COVID-19; COVID-19 patient; COVID-19 susceptibility; COVID-19 treatment; Catheters; Chronic; Clinical; Communicable Diseases; Communication; Computers; Coronavirus; Custom; Dangerousness; Data; Death Rate; Dexamethasone; Disease Outbreaks; Disease Vectors; Dose; Drug Industry; Drug Kinetics; Effectiveness; Environment; Exhibits; Exposure to; External Infusion Pumps; Genetic; Gold; Hemorrhage; Hormonal Change; Human; Implant; Implantable Pump; Infection; Infectious Agent; Inflammatory; Infusion Pumps; Infusion procedures; Intelligence; Intravenous; Invaded; Laboratories; Laboratory Animals; Link; Liquid substance; Long COVID; Long-Term Effects; Magnetic Resonance Imaging; Maintenance; Mammals; Manuals; Medical; Memory; Modeling; Mus; Mutate; Needles; Oral; Outcome; Oxygen; Pathogenicity; Patients; Pharmaceutical Preparations; Pharmacological Treatment; Phase; Productivity; Property; Protocols documentation; Protozoa; Public Health; Pump; Rattus; Reaction; Reproducibility; Research; Research Personnel; Risk; Rodent; SARS coronavirus; SARS-CoV-2 infection; Safety; Small Business Innovation Research Grant; Sterilization; Stress; System; Telemetry; Testing; Therapeutic; Time; Toxic effect; Toxicology; Training; Vaccines; Validation; Virus; Virus Diseases; Wireless Technology; Wistar Rats; World War II; biomaterial compatibility; combat; cost; drug candidate; drug development; experimental study; flexibility; fungus; in vivo; infection risk; instrument; intraperitoneal; microorganism; mouse model; novel; novel coronavirus; operation; pandemic disease; pandemic influenza; pathogenic virus; pre-clinical research; programs; response; restraint; safety testing; side effect; subcutaneous; targeted treatment; vaccine development; vaccine trial; vaccine-induced antibodies

Project Summary Infectious diseases are caused by micro-organisms, such as bacteria, protozoa, viruses or fungi, which can be transferred through direct or indirect human contact. A viral infection occurs when a host's body is invaded by pathogenic viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19 and the current pandemic. This pandemic is the greatest public health challenge since the 1918 influenza pandemic and the biggest threat to destabilizing the global economy since World War II. As viruses tend to mutate quicker than other pathogenic agents (and thus newer strains emerge time and again), continuous research is required to combat infectious agents. For preclinical research, the most frequently used animal models are mice and rats. They offer an optimal combination of genetic proximity to humans, cost for breeding and colony maintenance possibilities due to their small size. Mice offer the broadest spectrum of available models. Rats are the second most frequently used mammal animal model. In fact, several SARS-CoV- 2 researchers are turning to rats. They are no more susceptible to COVID-19 than mice, but their larger size is an advantage, as, for example, researchers often want to do repetitive bleeding in an experiment but cannot do that with mice. Furthermore, as vaccine studies often assess how different doses affect antibody responses over several days, most toxicology studies of drugs also start in rat. To achieve intermittent infusions in most non-infectious disease research, the current prevailing administration modes for small animal research are manual (oral, intravenous, intraperitoneal, subcutaneous) requiring repeated handling by trained technicians. However, infectious disease researchers desire the least number of touchpoints possible with their infected animals, especially when sharp needles are involved The proposed FluidSync BSL3 system may aid the discovery of new treatments for COVID-19 by enabling candidate drugs to be administered to model animals infected with SARS-CoV-2 while minimizing investigator contact. It may also be used in the development of vaccines and antibodies. The system builds on the first and only wireless and tether-free administration system that can be used in animals as small as mice. The new system will have new capabilities including i) a medical-grade primary battery and ii) a programmable system-on-chip including Bluetooth telemetry transceiver, processor and memory. Ultimately, the FluidSync BSL3 microinfusion system would enable an intelligent instrumented vivarium system that addresses many BSL3 user requirements with benefits including increased productivity, reduced researcher exposure to potentially toxic drugs and disease vectors, ease of management of large-scale animal studies, and minimized animal handling to reduce white coat effects.

项目摘要 传染病是由微生物引起的，如细菌、原生动物、病毒或真菌，它们可以是 通过直接或间接的人际接触传播。病毒感染发生在宿主的身体被 病原性病毒，如严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）， 导致COVID-19和当前的大流行。这一流行病是自2000年以来最大的公共卫生挑战。 1918年的流感大流行是二战以来破坏全球经济稳定的最大威胁。 由于病毒往往比其他病原体突变得更快（因此新的毒株一次又一次地出现）， 需要不断进行研究，以防治传染性病原体。对于临床前研究，最常用的 动物模型是小鼠和大鼠。它们提供了一个最佳的组合，基因接近人类， 繁殖和群体维持的可能性，由于他们的小尺寸。小鼠提供最广泛的 可用的模型。大鼠是第二种最常用的哺乳动物模型。事实上，几个SARS-CoV- 两名研究人员正在转向老鼠。它们并不比老鼠更容易感染COVID-19，但它们的体型更大， 这是一个优势，例如，研究人员经常想在实验中重复出血，但不能这样做。 那是老鼠。此外，由于疫苗研究经常评估不同剂量如何影响抗体反应， 几天后，大多数药物的毒理学研究也开始在大鼠中进行。 为了在大多数非传染性疾病研究中实现间歇性输注， 用于小动物研究的模式是手动的（口服、静脉内、腹膜内、皮下），需要 由经过培训的技术人员重复处理。然而，传染病研究人员希望最少数量的 接触点可能与他们感染的动物，特别是当涉及尖锐的针头 拟议的FluidSync BSL 3系统可以通过使 将候选药物给予感染SARS-CoV-2的模型动物，同时最小化 调查员联系人。它也可以用于疫苗和抗体的开发。该系统建立在 这是第一个也是唯一一个可以用于小到老鼠的动物的无线和无系绳管理系统。 新系统将具有新功能，包括i）医用级原电池和ii）可编程 片上系统包括蓝牙遥测收发器、处理器和存储器。 最终，FluidSync BSL 3微量输注系统将实现智能仪器化动物园系统 该解决方案可满足许多BSL 3用户的需求，其优势包括提高生产力、减少研究人员 暴露于潜在有毒药物和疾病媒介，易于管理大规模动物研究，以及 尽量减少动物处理，以减少白色被毛影响。