Commercialization of cavitation-enhancing nanodroplets for DNA sample fragmentation in NGS applications

用于 NGS 应用中 DNA 样品碎片化的空化增强纳米液滴的商业化

基本信息

  • 批准号:
    10259765
  • 负责人:
  • 金额:
    $ 115.47万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-07-05 至 2023-08-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY The completion of the Human Genome Project has established NGS as the gold standard technology for personalized/precision medicine and diagnostics. Genetic testing kits are now as readily available as pregnancy test kits, and advances in genetic analysis, gene therapy, and genetic modification in the 21st century promise to parallel the development of transistors and integrated circuits in the 20th century. The challenge is that technology for preparing biological samples for sequencing has not maintained a similar rapid advancement to gene sequencing. Fragmentation of DNA randomly without bias is an essential, but difficult step in the preparation of genomic DNA for NGS applications. A narrow range of DNA fragment lengths is required for sequencing, and inefficient or inconsistent fragmentation will result in incomplete or excluded reads, corrupting the sequencing. Sonication, which fragments DNA through cavitation, is the gold standard for DNA fragmentation. However, our customer discover interviews have reinforced that this is a significant pain point in their sample preparation process due to its low throughput and inconsistency, particularly for industry developing large-scale sequencing efforts. We have invented a novel nanodroplet reagent that enhances cavitation from an acoustic sonicator, and greatly improves consistency and reduces time and acoustic power requirements for DNA fragmentation. Triangle Biotechnology has licensed the patents describing this cavitation enhancing technology. We have achieved milestones proposed in Phase I, demonstrating product and technical feasibility. With this SBIR Phase II, we will perform key R&D tasks, product development, and validation studies to prepare for commercialization. Success of this project will enable a novel commercial solution for addressing market pain points: substantially improving throughput and consistency of DNA fragmentation for NGS sample preparation while reducing cost.
项目摘要 人类基因组计划的完成确立了NGS作为黄金标准技术, 个性化/精准医疗和诊断。基因检测试剂盒现在就像怀孕一样容易获得 检测试剂盒,以及21世纪遗传分析、基因治疗和遗传修饰的进展, 与世纪晶体管和集成电路的发展并行。挑战在于, 用于制备用于测序的生物样品的方法没有保持与基因测序类似的快速进展, 测序无偏倚地随机片段化DNA是制备DNA片段的基本但困难的步骤。 用于NGS应用的基因组DNA。测序需要窄范围的DNA片段长度, 低效或不一致的片段化将导致不完整或排除的读数,破坏测序。 通过空化作用使DNA片段化的超声处理是DNA片段化的金标准。 然而,我们的客户发现访谈进一步证实,这是他们样本中的一个重大痛点 由于其低通量和不一致性,特别是对于工业开发大规模的制备过程, 排序的努力。 我们已经发明了一种新的纳米液滴试剂,其增强了来自声学超声仪的空化,并且 大大提高了一致性并减少了DNA片段化的时间和声功率要求。 Triangle Biotechnology已授权描述这种空化增强技术的专利。 我们已经实现了第一阶段提出的里程碑,证明了产品和技术的可行性。与 在SBIR第二阶段,我们将执行关键的研发任务、产品开发和验证研究,为 商业化该项目的成功将为解决市场需求提供一种新的商业解决方案。 痛点:大幅提高NGS样品的通量和DNA片段化的一致性 同时降低成本。

项目成果

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Sandeep Kasoji其他文献

Sandeep Kasoji的其他文献

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{{ truncateString('Sandeep Kasoji', 18)}}的其他基金

Improving diagnostic sensitivity for difficult-to-lyse microbial samples with nanodroplet technology
利用纳米液滴技术提高难以裂解的微生物样品的诊断灵敏度
  • 批准号:
    10484601
  • 财政年份:
    2020
  • 资助金额:
    $ 115.47万
  • 项目类别:
Improving diagnostic sensitivity for difficult-to-lyse microbial samples with nanodroplet technology
利用纳米液滴技术提高难以裂解的微生物样品的诊断灵敏度
  • 批准号:
    10081308
  • 财政年份:
    2020
  • 资助金额:
    $ 115.47万
  • 项目类别:
Improving diagnostic sensitivity for difficult-to-lyse microbial samples with nanodroplet technology
利用纳米液滴技术提高难以裂解的微生物样品的诊断灵敏度
  • 批准号:
    10628013
  • 财政年份:
    2020
  • 资助金额:
    $ 115.47万
  • 项目类别:
Commercialization of cavitation-enhancing nanodroplets for DNA sample fragmentation in NGS applications
用于 NGS 应用中 DNA 样品碎片化的空化增强纳米液滴的商业化
  • 批准号:
    10081304
  • 财政年份:
    2018
  • 资助金额:
    $ 115.47万
  • 项目类别:

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