Micro-engineered capsules for spatial sampling of microbiome in vivo
用于体内微生物组空间采样的微工程胶囊
基本信息
- 批准号:10269448
- 负责人:
- 金额:$ 15.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-02-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:3D PrintAddressAdministrative SupplementAnimal ModelAnimalsAntibioticsAscending colonAutopsyAwardBudgetsCellsCelluloseChargeChronic DiseaseColorComplementary DNAComplexCustomDNADataDietDietary FiberDietary InterventionEcosystemEngineeringEnterocytesEpithelial CellsEscherichia coliExploratory/Developmental Grant for Diagnostic Cancer ImagingFamily suidaeFecesFundingGastrointestinal tract structureGene ExpressionGenerationsGenesGenetic TranscriptionGoalsHealthHigh-Throughput Nucleotide SequencingHumanIn SituInflammatoryIntestinesInulinKnowledgeLaboratoriesLinkLocationMetagenomicsMethodsModelingMotorOrganParentsPectinsPhysiologicalPopulationPostdoctoral FellowPumpRNAResearchResearch PersonnelResearch ProposalsRiskRodentRunningSamplingStructureSuspensionsTechnologyTestingTrainingWeaningbody systemcapsulecolon microbiotacostdesigndietarydietary controldietary supplementsdysbiosisexperimental studyfeasibility testinggraduate studentgut microbiomegut microbiotaileumimprovedin vitro testingin vivoinsoluble fiberintestinal epitheliumjejunummagnetic fieldmicrobialmicrobiomemicrobiotamotor controlnovelpandemic diseaseparent projectprebioticsprototyperRNA Genesresponsesoluble fibertranscriptometranscriptome sequencing
项目摘要
ABSTRACT OF SUPPLEMENTAL RESEARCH
This proposal is submitted in response to PA-20-227 (Administrative Supplements for Research on Dietary
Supplements). The Specific Aims of parent award R21 AI144521 are (1) to design, fabricate and test micro-
engineered ingestible capsules for sampling the gastro-intestinal tract; (2) to test the capsules in pigs.
The parent project seeks to demonstrate the feasibility of non-invasively sampling the gastro-intestinal tract in
a realistic animal model and to validate the ingestible capsule technology. Non-invasive sampling of the
gastro-intestinal tract will advance the study of the intestinal microbiome and its beneficial functions. We are
seeking supplemental funding to leverage research conducted under the parent award to study the effect of
dietary supplements on the bacterial microbiota populating the gastro-intestinal tract. Although the intestinal
microbiota's response to various perturbations, like diet, antibiotics or inflammatory conditions has been
studied, the bulk of this research has been conducted in humans and rodents. For obvious reasons, most of
these studies rely on the analysis of fecal DNA and fecal metabolites, which do not closely mirror changes in
more proximal sections of the GI tract, like the jejunum, ileum and ascending colon. Supplemental funding
would allow us to: 1) increase the number of pigs to study the effect of 3 commonly used prebiotic
supplements (inulin, pectin, cellulose) on the microbiota populating different organs of the GI tract, 2) expand
the scope of the project beyond the bacterial microbiota to the intestinal epithelium and its transcriptional
response to diet. To non-invasively investigate the response of the intestinal epithelium to changes in diet, we
are proposing to use RNA-Seq to analyze the "exfoliome", i.e., the transcriptome of exfoliated epithelial cells
excreted in feces. To assess the quality of the exfoliome, we will compare exfoliome RNA-Seq data with RNA-
Seq data obtained from epithelial cells collected directly from different GI organs after the animal has been
sacrificed. The analysis of the pig intestinal exfoliome seeks to evaluate whether changes in enterocyte gene
expression are detectable non-invasively by sequencing fecal cDNA. Although supplementary experiments
expand the scope of the parent award to include dietary intervention, this research is not dependent on the
optimization of the ingestible capsule. Pigs used for testing the capsules will be leveraged to investigate the
following Supplemental Specific Aims: 1) to assess the impact of inulin, pectin and cellulose on the
bacterial microbiota sampled in vivo using an ingestible capsule and collected post-mortem from
different GI organs; 2) to apply RNA-Seq to evaluate the feasibility of characterizing the transcriptome
of exfoliated enterocytes from pigs fed control and test diet. Thus, supplemental experiments will use a
well-defined dietary intervention to expand the scope of the parent award in two directions; 1) to assess the
effect of diet on commensal bacterial which are not present in fecal DNA; 2) to test the feasibility of non-
invasively characterizing the enterocyte transcriptome and its response to dietary perturbation.
补充研究摘要
本提案是根据PA-20-227(膳食研究行政补充资料)提交的
补充剂)。家长奖R21 AI144521的具体目标是:(1)设计、制造和测试微处理器
设计了用于胃肠道采样的可食用胶囊;(2)在猪身上进行试验。
母公司的项目旨在证明非侵入性胃肠道采样的可行性。
建立了逼真的动物模型,并验证了可食胶囊技术。非侵入性采样
胃肠道将促进对肠道微生物及其有益功能的研究。我们是
寻求补充资金,以利用在家长奖下进行的研究,以研究
膳食补充剂对胃肠道细菌微生物区系的影响。虽然肠道
微生物区系对各种扰动的反应,如饮食、抗生素或炎症条件
在研究中,这项研究的大部分是在人类和啮齿动物身上进行的。出于显而易见的原因,大多数
这些研究依赖于对粪便DNA和粪便代谢物的分析,这些分析并不能很好地反映
胃肠道更近端的部分,如空肠、回肠和升结肠。补充资金
可以让我们:1)增加猪的数量来研究3种常用的益生素的效果
补充剂(菊粉、果胶、纤维素)对胃肠道不同器官中微生物区系的影响,2)扩大
该项目的范围从细菌微生物区系到肠道上皮细胞及其转录
对饮食的反应。为了非侵入性地研究肠道上皮对饮食变化的反应,我们
正在提议使用RNA-Seq来分析“剥离体”,即脱落的上皮细胞的转录组
从粪便中排出。为了评估剥离体的质量,我们将剥离体RNA-Seq数据与RNA-Seq数据进行比较。
直接从不同的胃肠道器官采集的上皮细胞的SEQ数据
牺牲了。对猪肠道脱落细胞的分析试图评估肠细胞基因的变化
通过对粪便DNA测序,可以非侵入性地检测到表达。虽然补充实验
扩大家长奖励的范围,包括饮食干预,这项研究不依赖于
口服胶囊剂的优化。用来测试胶囊的猪将被用来调查
以下补充具体目标:1)评估菊粉、果胶和纤维素对
使用可食用胶囊在体内采集细菌微生物区系,并在死后从
2)应用RNA-Seq技术鉴定转录组的可行性
饲喂对照日粮和试验日粮的猪的脱落肠细胞。因此,补充实验将使用
明确的饮食干预措施,从两个方向扩大家长奖励的范围;1)评估
饮食对粪便DNA中不存在的共生菌的影响;2)测试非
肠道细胞转录组的侵袭性特征及其对饮食扰动的反应。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Impact of Physical Effort on the Gut Microbiota of Long-Distance Fliers.
- DOI:10.3390/microorganisms11071766
- 发表时间:2023-07-06
- 期刊:
- 影响因子:4.5
- 作者:
- 通讯作者:
Effect of Caging on Cryptosporidium parvum Proliferation in Mice.
- DOI:10.3390/microorganisms10061242
- 发表时间:2022-06-17
- 期刊:
- 影响因子:4.5
- 作者:
- 通讯作者:
The association between fecal microbiota, age and endoparasitism in adult alpacas.
- DOI:10.1371/journal.pone.0272556
- 发表时间:2022
- 期刊:
- 影响因子:3.7
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Sameer R Sonkusale其他文献
Sameer R Sonkusale的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Sameer R Sonkusale', 18)}}的其他基金
Ingestible Pill for spatially targeted sampling of gut microbiome
用于肠道微生物组空间靶向采样的可摄入药丸
- 批准号:
10642791 - 财政年份:2022
- 资助金额:
$ 15.99万 - 项目类别:
Ingestible Pill for spatially targeted sampling of gut microbiome
用于肠道微生物组空间靶向采样的可摄入药丸
- 批准号:
10424745 - 财政年份:2022
- 资助金额:
$ 15.99万 - 项目类别:
Micro-engineered capsules for spatial sampling of microbiome in vivo
用于体内微生物组空间采样的微工程胶囊
- 批准号:
9979087 - 财政年份:2020
- 资助金额:
$ 15.99万 - 项目类别:
Micro-engineered capsules for spatial sampling of microbiome in vivo
用于体内微生物组空间采样的微工程胶囊
- 批准号:
10088394 - 财政年份:2020
- 资助金额:
$ 15.99万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 15.99万 - 项目类别:
Research Grant