Defining mechanisms of microbe-microbe interactions in chronic wound infection

项目摘要 金黄色葡萄球菌是全球人类感染的主要原因。葡萄球菌的调查研究 金黄色葡萄球菌的发病机制主要集中在单种感染和混合感染微生物对金黄色葡萄球菌的影响。 金黄色葡萄球菌在感染过程中的生理学仍未得到充分的研究，但至关重要。我最近的工作主要集中在 用NEXT-PCR技术研究铜绿假单胞菌单一感染和混合感染时金黄色葡萄球菌的生理特征 世代排序技术。当前的应用程序在逻辑上建立在这项工作的基础上，重点放在 描述慢性伤口感染中金黄色葡萄球菌和共感染微生物之间的相互作用。 这个应用程序的总体目标有两个：1)定义S。 金黄色葡萄球菌和感染过程中的共同感染微生物(具体目标1)；和2)扩大这些发现，以 在人类感染中确定微生物-微生物相互作用对金黄色葡萄球菌生理的影响(特定 目标2)。这项工作可能会产生重要的发现，这将有助于我们理解潜在的 慢性多菌感染中微生物-微生物相互作用的机制。

Project Summary Staphylococcus aureus is a leading cause of human infection worldwide. Investigations into Staphylococcus aureus pathogenesis have focused on mono-species infections and the impact of co-infecting microbes on S. aureus physiology during infection remains understudied but critically important. My recent work has focused on characterizing S. aureus physiology during mono- and co-infection with Pseudomonas aeruginosa using next- generation sequencing techniques. The current application builds logically on this work and is focused on characterizing the interactions between S. aureus and co-infecting microbes within chronic wound infections. The overall goal of this application is two-fold: 1) to define the metabolic interactions that occur between S. aureus and co-infecting microbes during infection (Specific Aim 1); and 2) to expand on these findings to determine the impact of microbe-microbe interactions on S. aureus physiology within human infections (Specific Aim 2). This work is likely to yield important discoveries that will aid in our understanding of the underlying mechanisms of microbe-microbe interactions within chronic polymicrobial infections.