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Defining mechanisms of microbe-microbe interactions in chronic wound infection

慢性伤口感染中微生物-微生物相互作用的定义机制

基本信息

批准号：
10569495
负责人：
Carolyn Brook Ibberson
金额：
$ 10.51万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-09 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10569495
关键词：
Address Affect American Animal Model Chronic Coculture Techniques Communities Complex Confocal Microscopy Data Disease Disease Outcome Economic Burden Electron Transport Environment Fermentation Gene Expression Global Change Goals Healthcare Systems Human In Situ In Vitro Infection Investigation Mediating Metabolic Metabolism Microbe Molecular Mus Organism Pathogenesis Physiology Process Pseudomonas aeruginosa Role Severity of illness Staphylococcus aureus Structure System Techniques Testing Work Wound Infection Wound models analysis pipeline antibiotic tolerance chronic wound co-infection design fitness genetic approach healing improved in vitro Model in vivo inhibitor microbial community microorganism interaction next generation sequencing novel therapeutic intervention opportunistic pathogen pathogen response synergism transcriptome transcriptome sequencing wound

项目摘要

Project Summary Staphylococcus aureus is a leading cause of human infection worldwide. Investigations into Staphylococcus aureus pathogenesis have focused on mono-species infections and the impact of co-infecting microbes on S. aureus physiology during infection remains understudied but critically important. My recent work has focused on characterizing S. aureus physiology during mono- and co-infection with Pseudomonas aeruginosa using next- generation sequencing techniques. The current application builds logically on this work and is focused on characterizing the interactions between S. aureus and co-infecting microbes within chronic wound infections. The overall goal of this application is two-fold: 1) to define the metabolic interactions that occur between S. aureus and co-infecting microbes during infection (Specific Aim 1); and 2) to expand on these findings to determine the impact of microbe-microbe interactions on S. aureus physiology within human infections (Specific Aim 2). This work is likely to yield important discoveries that will aid in our understanding of the underlying mechanisms of microbe-microbe interactions within chronic polymicrobial infections.

项目摘要金黄色葡萄球菌是全球人类感染的主要原因。葡萄球菌调查金黄色葡萄球菌的致病机制主要集中在单种感染和共感染微生物对金黄色葡萄球菌的影响。金黄色葡萄球菌在感染过程中的生理学仍然研究不足，但至关重要。我最近的工作主要集中在表征S.金黄色葡萄球菌生理学在单一和共同感染铜绿假单胞菌使用next- 代测序技术。目前的应用程序逻辑上建立在这项工作，并侧重于描述了S.金黄色葡萄球菌和慢性伤口感染中的共感染微生物。本申请的总体目标是双重的：1）定义S. 金黄色葡萄球菌和共感染的微生物在感染（具体目标1）;和2）扩大这些发现，确定微生物-微生物相互作用对S.人类感染中的金黄色葡萄球菌生理学（特异性目标2）。这项工作很可能会产生重要的发现，有助于我们了解潜在的慢性多微生物感染中微生物-微生物相互作用的机制。