Engineering an immuno-isolating hydrogel for restoring ovarian endocrine function

项目摘要 卵巢功能不全(POI)是抗癌治疗的常见并发症，如 作为化疗和骨髓移植，由于治疗的毒性。在女性癌症中 幸存者POI导致不孕不育和卵巢内分泌功能丧失，进而导致 骨质疏松症、肌肉萎缩和心血管疾病加速。这些长长的- 持久的影响是显著的，特别是对进入青春期的年轻女孩。的长期目标是 我们的研究旨在开发一种方法来恢复患有卵巢癌的年轻女性的卵巢内分泌功能 利用同种异体卵巢组织移植的POI。我们设计了一种免疫隔离 胶囊以特性良好的生物材料为基础，并根据卵巢的独特生理条件进行调整 组织。我们的方法在使用啮齿动物供体组织的小鼠身上被证明是成功的，然而 人卵巢组织中间质和卵泡的比例有显著差异，其大小 人类卵泡成熟时，休眠卵泡和激活卵泡之间的平衡 啮齿类动物和人类之间的关系需要使用人类卵巢组织进行进一步研究。如果 如果成功，这种方法将提供一种临床上相关且未被探索的修复卵巢的工具 POI年轻女性的内分泌功能。

Project Summary Premature ovarian insufficiency (POI) is a common complication of anticancer treatments, such as chemotherapy and bone marrow transplantation, due to treatment toxicity. In female cancer survivors POI causes sterility, and loss of the ovarian endocrine function, which in turn results in premature osteopenia, muscle wasting, and accelerated cardiovascular disease. These long- lasting effects are significant, particularly for young girls reaching puberty. The long-term goal of our research is to develop a means to restore ovarian endocrine function in young women with POI by utilizing allogeneic transplantation of ovarian tissue. We designed an immuno-isolating capsule based on well-characterized biomaterials and tuned to the unique physiology of ovarian tissue. Our approach proved to be successful in mice using donor tissue from rodents, yet the significant differences in ratio between stroma and follicles in human ovarian tissue, size that human follicles reach at maturity and the balance between dormant and activated follicles between rodents and humans require further investigation using human ovarian tissue. If successful, this approach will offer a clinically relevant and unexplored tool to restore ovarian endocrine function in young women with POI.